##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062892_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	388301
Sequences flagged as poor quality	0
Sequence length	38
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.701839552306073	32.0	32.0	32.0	32.0	32.0
2	30.456231119672626	32.0	32.0	32.0	32.0	32.0
3	30.613719768942136	32.0	32.0	32.0	32.0	32.0
4	30.57447186589785	32.0	32.0	32.0	32.0	32.0
5	30.633397802220443	32.0	32.0	32.0	32.0	32.0
6	34.16476908377779	36.0	36.0	36.0	32.0	36.0
7	33.93941555648839	36.0	36.0	36.0	32.0	36.0
8	33.828679297761276	36.0	36.0	36.0	32.0	36.0
9	33.86153782761311	36.0	36.0	36.0	32.0	36.0
10	33.76123677250381	36.0	36.0	36.0	32.0	36.0
11	34.149839943754976	36.0	36.0	36.0	32.0	36.0
12	33.96138305077762	36.0	36.0	36.0	32.0	36.0
13	34.03938439509556	36.0	36.0	36.0	32.0	36.0
14	33.959433532234016	36.0	36.0	36.0	32.0	36.0
15	33.986281261186555	36.0	36.0	36.0	32.0	36.0
16	33.94775187290272	36.0	36.0	36.0	32.0	36.0
17	33.88693822575785	36.0	36.0	36.0	32.0	36.0
18	33.99031679032503	36.0	36.0	36.0	32.0	36.0
19	33.880221786706706	36.0	36.0	36.0	32.0	36.0
20	33.70172881347202	36.0	36.0	36.0	27.0	36.0
21	33.66406988393025	36.0	36.0	36.0	27.0	36.0
22	33.718434925483066	36.0	36.0	36.0	27.0	36.0
23	33.766874151753406	36.0	36.0	36.0	32.0	36.0
24	33.759034872431435	36.0	36.0	36.0	27.0	36.0
25	33.79805614716418	36.0	36.0	36.0	32.0	36.0
26	33.79191142953533	36.0	36.0	36.0	32.0	36.0
27	33.85376808197764	36.0	36.0	36.0	32.0	36.0
28	33.82041509035516	36.0	36.0	36.0	32.0	36.0
29	33.78174405937662	36.0	36.0	36.0	27.0	36.0
30	33.733000945143075	36.0	36.0	36.0	27.0	36.0
31	33.72052866204311	36.0	36.0	36.0	27.0	36.0
32	33.65847886047165	36.0	36.0	36.0	27.0	36.0
33	33.525996585123394	36.0	36.0	36.0	21.0	36.0
34	33.45175005987623	36.0	36.0	36.0	21.0	36.0
35	33.42513926052212	36.0	36.0	36.0	21.0	36.0
36	33.39284730144913	36.0	36.0	36.0	21.0	36.0
37	33.41720726961816	36.0	36.0	36.0	21.0	36.0
38	32.689071107208065	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	4.0
9	0.0
10	4.0
11	4.0
12	4.0
13	4.0
14	179.0
15	337.0
16	425.0
17	572.0
18	874.0
19	1067.0
20	1270.0
21	1398.0
22	1723.0
23	2166.0
24	2830.0
25	3691.0
26	4766.0
27	6235.0
28	8388.0
29	10998.0
30	14637.0
31	19613.0
32	26291.0
33	38909.0
34	83762.0
35	158147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.829494941160696	18.63803257046793	12.255953547375695	21.27651894099568
2	15.58092852707066	21.256348611229214	40.553034578189376	22.609688283510746
3	19.189588071511178	28.831130697725932	29.178847486265187	22.800433744497703
4	10.662123565149981	16.315503462698405	37.43603215197241	35.5863408201792
5	12.273983327367171	40.40319236880667	31.420727734412225	15.902096569413935
6	28.746072624259593	39.25547257275303	18.438578418748392	13.55987638423899
7	27.005081109757633	34.53017118163487	19.748339561319696	18.7164081472878
8	29.298449382817793	31.369029376997503	18.65942822118121	20.673093019003495
9	29.514111548059613	12.752286253634443	19.886479233786336	37.847122964519606
10	17.03592868345622	27.627048367615508	29.519671797618834	25.81735115130944
11	35.24657925844659	24.325702497856	22.083323675677903	18.344394568019514
12	25.330685786692214	27.082753860576283	28.880922211577094	18.705638141154413
13	29.46179504681443	21.809986219686532	27.278066119747063	21.45015261375198
14	24.26170227013996	20.77175486403371	28.15447460193995	26.812068263886385
15	25.56221309743997	26.76546504618777	26.65289362644836	21.019428229923907
16	23.063173050423565	28.576616673234582	25.506695410958375	22.853514865383477
17	20.192419149182797	26.372757363831894	27.621292325129115	25.81353116185619
18	20.24910564582802	27.3637606916854	30.832489845957312	21.55464381652926
19	23.68414274823316	24.445222838068943	30.726773535532526	21.14386087816537
20	24.823255801974383	24.48895493634633	29.51711811019566	21.17067115148362
21	27.88386252652834	22.50710856530608	28.303164856901493	21.305864051264088
22	24.02533347413209	24.424291906732844	29.530908332281637	22.019466286853433
23	23.91545675349194	25.119059577438307	29.63984638803248	21.325637281037267
24	22.83332131340085	26.359969503714158	29.303221685331906	21.50348749755309
25	23.290652778099748	25.829334446067183	29.896222114039766	20.983790661793307
26	22.138649260593944	26.508370318661985	30.88092484357975	20.47205557716432
27	21.376782664911797	26.879637761974795	30.84509327680662	20.89848629630679
28	20.882621853068056	27.891056596484447	30.286781276157367	20.93954027429013
29	20.82504816558659	26.304591957634887	30.091128259548118	22.7792316172304
30	21.35462004810887	28.032944788120094	29.109213311837152	21.503221851933883
31	21.66701436912608	28.20279037662615	28.33800799973214	21.792187254515625
32	20.497545497159166	28.56134711769766	29.088047884697605	21.85305950044557
33	21.946175104762542	27.621767943563448	28.424582559580074	22.007474392093936
34	21.6926556117061	27.474616347535814	29.505308544670594	21.32741949608749
35	21.81707656325664	28.292137609858127	28.06881523228618	21.82197059459905
36	21.655329090220665	29.498734020714153	27.597575707621893	21.248361181443293
37	21.688087572440438	29.58480360592402	27.52530585962653	21.201802962009015
38	21.053200914841447	30.24849071765603	28.189582346032598	20.50872602146993
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	89.0
1	181.5
2	274.0
3	274.0
4	580.5
5	887.0
6	887.0
7	1263.5
8	1640.0
9	1856.0
10	2072.0
11	2072.0
12	2692.5
13	3313.0
14	4137.0
15	4961.0
16	4961.0
17	6807.0
18	8653.0
19	8653.0
20	9471.5
21	10290.0
22	8850.0
23	7410.0
24	7410.0
25	7815.0
26	8220.0
27	8220.0
28	10739.0
29	13258.0
30	15505.0
31	17752.0
32	17752.0
33	18172.0
34	18592.0
35	18592.0
36	19107.5
37	19623.0
38	22103.0
39	24583.0
40	24583.0
41	24946.5
42	25310.0
43	26765.0
44	28220.0
45	28220.0
46	27629.0
47	27038.0
48	27038.0
49	27178.0
50	27318.0
51	25236.5
52	23155.0
53	23155.0
54	22710.5
55	22266.0
56	22266.0
57	21344.0
58	20422.0
59	19104.0
60	17786.0
61	17786.0
62	16665.0
63	15544.0
64	14201.0
65	12858.0
66	12858.0
67	11260.0
68	9662.0
69	9662.0
70	8086.0
71	6510.0
72	5377.0
73	4244.0
74	4244.0
75	3448.5
76	2653.0
77	2653.0
78	2060.5
79	1468.0
80	1160.0
81	852.0
82	852.0
83	699.0
84	546.0
85	546.0
86	394.5
87	243.0
88	200.0
89	157.0
90	157.0
91	106.0
92	55.0
93	52.0
94	49.0
95	49.0
96	43.0
97	37.0
98	37.0
99	164.0
100	291.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11975246007607501
2	0.057687206574281294
3	0.013906737299157097
4	0.005150643444132258
5	0.0
6	2.575321722066129E-4
7	0.0
8	0.0010301286888264516
9	5.150643444132258E-4
10	0.0010301286888264516
11	0.002060257377652903
12	0.004378046927512419
13	0.01699712336563645
14	0.011073883404884355
15	0.02652581373728113
16	0.012876608610330645
17	0.02214776680976871
18	0.008241029510611612
19	0.009528690371644678
20	0.007210900821785162
21	0.009528690371644678
22	0.010301286888264516
23	0.012876608610330645
24	0.014679333815776935
25	0.017512187710049677
26	0.021632702465355483
27	0.01236154426591742
28	0.0066958364773719355
29	0.014679333815776935
30	0.0038629825830991935
31	0.009786222543851291
32	0.009013626027231452
33	0.010816351232677742
34	0.015194398160190163
35	0.01879984857108274
36	0.016482059021223225
37	0.013134140782537258
38	0.009528690371644678
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	388301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.234073932670846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.21569260299695	22.9781453474433
2	13.965065475943886	9.84092298907992
3	6.777666963928697	7.164144566942533
4	3.909702771048434	5.510190259595544
5	2.5460491400879715	4.485384181903631
6	1.6256394219510109	3.4366739748531723
7	1.1492894190716945	2.8345903853125884
8	0.7973183416154807	2.2474218717083483
9	0.6241161929517588	1.97911404765354
>10	3.0442411026547633	18.63826890251933
>50	0.1941398287977303	4.720861208100814
>100	0.1386713062840931	8.655340807400435
>500	0.006568640823983356	1.5835637498357564
>1k	0.005108942863098167	4.108573892680396
>5k	7.298489804425952E-4	1.8168038149707078
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7065	1.8194647966397204	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4850	1.2490310352020726	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2625	0.6760219520423589	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2571	0.6621152147432018	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2234	0.5753268727095733	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1495	0.3850105974488863	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1105	0.28457305028830726	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	1097	0.28251279291065434	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	917	0.23615700191346403	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	832	0.21426676727590194	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	764	0.1967545795658523	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	739	0.19031627526068692	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	659	0.16971370148415793	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	588	0.1514289172574884	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	572	0.14730840250218258	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	563	0.14499061295232307	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	524	0.13494685823626518	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG	442	0.1138292201153229	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	432	0.11125389839325678	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	424	0.10919364101560386	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	412	0.10610325494912452	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	404	0.1040429975714716	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC	400	0.10301286888264516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.575321722066129E-4	0.0
10	0.0	0.0	0.0	5.150643444132258E-4	0.0
11	0.0	0.0	0.0	7.725965166198388E-4	0.0
12	2.575321722066129E-4	0.0	0.0	7.725965166198388E-4	0.0
13	2.575321722066129E-4	0.0	0.0	7.725965166198388E-4	0.0
14	2.575321722066129E-4	0.0	0.0	7.725965166198388E-4	0.0
15	2.575321722066129E-4	0.0	0.0	7.725965166198388E-4	0.0
16	2.575321722066129E-4	0.0	0.0	0.0010301286888264516	0.0
17	2.575321722066129E-4	0.0	0.0	0.0010301286888264516	0.0
18	2.575321722066129E-4	0.0	0.0	0.0010301286888264516	0.0
19	2.575321722066129E-4	0.0	0.0	0.0012876608610330647	0.0
20	2.575321722066129E-4	0.0	0.0	0.0015451930332396775	0.0
21	2.575321722066129E-4	0.0	0.0	0.002317789549859516	0.0
22	2.575321722066129E-4	0.0	0.0	0.002832853894272742	0.0
23	2.575321722066129E-4	0.0	0.0	0.005150643444132259	0.0
24	2.575321722066129E-4	0.0	0.0	0.008241029510611612	0.0
25	2.575321722066129E-4	0.0	0.0	0.010301286888264517	0.0
26	2.575321722066129E-4	0.0	0.0	0.010558819060471129	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCGAG	20	0.0037518842	31.997555	31
GACCGTT	30	2.2436549E-5	31.997553	7
TGCACTG	20	0.0037542512	31.993433	5
GGTATAG	30	8.334888E-4	26.705898	1
TATTAGG	30	8.366339E-4	26.688686	2
AGATTAG	30	8.410535E-4	26.664629	8
CCGTTAT	30	8.410535E-4	26.664629	9
CTACTAG	40	1.5751127E-4	24.035309	1
TGAACTT	40	1.591728E-4	23.998167	10
TACGGCC	40	1.591728E-4	23.998167	19
GTAGCGA	60	1.7583989E-7	23.998165	9
GGTAAGT	55	2.2137156E-6	23.270948	28
TCGGGTA	55	2.2137156E-6	23.270948	25
AAGTTCC	55	2.2137156E-6	23.270948	31
TAAGGTA	55	2.2161948E-6	23.26795	5
TCGCCCC	35	0.0020650097	22.855394	25
GAACTTA	35	0.0020650097	22.855394	11
CCTAAGG	85	4.274625E-10	22.592327	3
TAGTACT	50	2.7930988E-5	22.398289	4
GCATGGG	65	3.8058533E-7	22.152153	9
>>END_MODULE