FastQCFastQC Report
Thu 2 Feb 2017
SRR4062892_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062892_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388301
Sequences flagged as poor quality0
Sequence length38
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT83062.139062222348127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT61771.590776227720248No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT39031.00514806812241No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26940.6937916719246152No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA24630.6343017401448876No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA15340.3950543521649442No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA13610.35050128637320016No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA12830.33041377694108437No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA10490.27015124864473694No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT9510.24491309576848888No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8600.22147766809768712No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA8020.20654080210970355No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA7160.18439303529993484No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG6840.17615200578932322No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA5940.15297411029072805No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT5580.14370295209129No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.12644829655344694No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA4670.12026752442048823No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA4630.11923739573166177No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4540.11691960618180226No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA4290.11048130187663693No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT4210.10842104449898403No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT4020.10352793322705839No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC4000.10301286888264516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGGT350.002062933322.859355
GAAAGCT350.002064478622.8564074
CACACGT350.002064478622.85640718
GGCTTGG453.528642E-421.3353967
GTTATAT707.749877E-720.5734161
GGATAGA400.00447572520.0019321
ACGTCAT400.004479062319.99935721
GTAACCA400.004479062319.99935732
GAACTTA507.179535E-419.20185511
AAGACTG507.179535E-419.2018555
TAAGTTC507.18576E-419.19938330
CTTATAG601.13599934E-418.6660652
AACGGGA802.7873775E-617.9994230
GCGAAAC450.008835117.77949511
TAGCCTG450.008835117.7794955
GTTGTGC450.008835117.7794951
TAATACA450.00884165817.7772064
ACACGTC450.00884165817.77720619
CTCCAGT450.00884165817.77720619
ATAGAGG450.00884165817.7772063
Produced by FastQC (version 0.11.5)