##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062892_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	388301
Sequences flagged as poor quality	0
Sequence length	38
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.65756462126031	32.0	32.0	32.0	32.0	32.0
2	31.21917790579988	32.0	32.0	32.0	32.0	32.0
3	31.332193324251033	32.0	32.0	32.0	32.0	32.0
4	31.466679199899048	32.0	32.0	32.0	32.0	32.0
5	31.273120594590278	32.0	32.0	32.0	32.0	32.0
6	34.76935676189348	36.0	36.0	36.0	32.0	36.0
7	34.85738898431887	36.0	36.0	36.0	32.0	36.0
8	34.83438363537565	36.0	36.0	36.0	32.0	36.0
9	34.93699217874793	36.0	36.0	36.0	32.0	36.0
10	34.757363488633814	36.0	36.0	36.0	32.0	36.0
11	34.977991300563225	36.0	36.0	36.0	36.0	36.0
12	34.87375772918432	36.0	36.0	36.0	32.0	36.0
13	34.930368451278774	36.0	36.0	36.0	36.0	36.0
14	34.845686722413795	36.0	36.0	36.0	32.0	36.0
15	34.81787582313721	36.0	36.0	36.0	32.0	36.0
16	34.80517433640397	36.0	36.0	36.0	32.0	36.0
17	34.78554780956011	36.0	36.0	36.0	32.0	36.0
18	34.77113115855998	36.0	36.0	36.0	32.0	36.0
19	34.73002387323236	36.0	36.0	36.0	32.0	36.0
20	34.68008065907634	36.0	36.0	36.0	32.0	36.0
21	34.68289806104028	36.0	36.0	36.0	32.0	36.0
22	34.69530853641891	36.0	36.0	36.0	32.0	36.0
23	34.68563820335255	36.0	36.0	36.0	32.0	36.0
24	34.65605290740946	36.0	36.0	36.0	32.0	36.0
25	34.663802050471155	36.0	36.0	36.0	32.0	36.0
26	34.605324220128196	36.0	36.0	36.0	32.0	36.0
27	34.61495849869045	36.0	36.0	36.0	32.0	36.0
28	34.57772707255454	36.0	36.0	36.0	32.0	36.0
29	34.532035714561644	36.0	36.0	36.0	32.0	36.0
30	34.5116185639491	36.0	36.0	36.0	32.0	36.0
31	34.44588090167164	36.0	36.0	36.0	32.0	36.0
32	34.39289623256185	36.0	36.0	36.0	32.0	36.0
33	34.28409919109145	36.0	36.0	36.0	32.0	36.0
34	34.22030847203587	36.0	36.0	36.0	32.0	36.0
35	34.1724383918661	36.0	36.0	36.0	32.0	36.0
36	34.12028297635082	36.0	36.0	36.0	32.0	36.0
37	34.12842099299255	36.0	36.0	36.0	32.0	36.0
38	33.73702617299466	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	1.0
21	10.0
22	33.0
23	67.0
24	244.0
25	637.0
26	1436.0
27	2835.0
28	4942.0
29	7982.0
30	12320.0
31	17588.0
32	25097.0
33	39521.0
34	92047.0
35	183539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.24355913388605	20.121927662435	12.944318670591842	21.69019453308711
2	15.192595434984716	22.161158482723454	39.21133347583447	23.434912606457363
3	18.62215137226018	28.718957715792648	29.755524708924263	22.903366203022912
4	10.416972487696336	16.873594832847882	37.292138274886106	35.417294404569674
5	11.724667204050467	40.236826585561204	31.676457181413387	16.362049028974944
6	28.999227401493687	39.546999742467165	17.503476693278394	13.950296162760752
7	26.393388582995534	34.544344807338696	19.92361536757851	19.138651242087263
8	29.06858755781731	31.4647224253912	18.587815355454143	20.878874661337342
9	28.860318735770722	12.651821862348179	19.86612891594812	38.62173048593298
10	16.983221519720832	27.727372229876767	29.72636783888538	25.563038411517013
11	35.371321872384264	23.943339128195223	21.887322129933683	18.798016869486833
12	24.771504580209683	27.388288982001075	28.839740304557548	19.000466133231694
13	29.512747875354105	21.640999227401494	27.559618851403556	21.286634045840845
14	23.990151969734818	20.884313972923067	28.38777134233494	26.737762715007175
15	25.868334101637647	26.580925622133343	26.397820247694444	21.152920028534563
16	23.052477330730543	27.99400465103103	25.838460369661682	23.115057648576748
17	20.10167370158717	26.008689135490254	28.11195438590166	25.77768277702092
18	20.39216999183623	26.272144547657618	31.41171410838499	21.923971352121164
19	23.761463400815348	23.93761540660467	31.237622359973322	21.063298832606662
20	24.37645022804474	24.206221462216167	30.4112016193623	21.006126690376796
21	27.354037203097597	22.034710186170006	29.412491855545053	21.198760755187344
22	23.615442659174196	23.791852197135725	30.68573091493455	21.906974228755526
23	23.648149245044436	24.23351987247007	30.72564840162657	21.39268248085892
24	22.468909428510354	25.50753152837618	30.212901846763206	21.810657196350252
25	23.127023247548927	25.15303928158455	30.69335743846881	21.026580032397714
26	21.694510186685072	25.86447111905455	31.82144779436571	20.61957089989467
27	21.237960339943342	25.809168168941536	31.853206283801182	21.099665207313933
28	20.71537286795553	26.704026000448106	31.43205914998931	21.148541981607053
29	20.71846325402201	25.075650075585692	31.17426944561049	23.031617224781805
30	21.35946083064427	26.716130012541818	30.24689609349448	21.677513063319434
31	21.60257120120731	26.827641443107282	29.51215680618901	22.057630549496395
32	20.45449277751023	27.187156355507714	30.357377395371117	22.00097347161094
33	21.939165750281354	26.2520570382255	29.78745869827788	22.021318513215263
34	21.49621041408598	26.32442357861556	30.715604646910517	21.463761360387945
35	21.909292018305386	26.996067483730407	29.247413733160617	21.847226764803594
36	21.70584160226216	28.03469473423967	28.73518224264166	21.5242814208565
37	21.634505190560933	28.413782091727807	28.65251441536334	21.29919830234792
38	21.091736007561185	28.685111215841403	29.61248934455149	20.61066343204592
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	37.5
2	59.0
3	59.0
4	272.5
5	486.0
6	486.0
7	931.5
8	1377.0
9	1804.5
10	2232.0
11	2232.0
12	2809.0
13	3386.0
14	4323.0
15	5260.0
16	5260.0
17	7608.5
18	9957.0
19	9957.0
20	10485.5
21	11014.0
22	9525.0
23	8036.0
24	8036.0
25	7863.5
26	7691.0
27	7691.0
28	10218.0
29	12745.0
30	14930.5
31	17116.0
32	17116.0
33	17584.0
34	18052.0
35	18052.0
36	18449.5
37	18847.0
38	21439.5
39	24032.0
40	24032.0
41	24798.5
42	25565.0
43	27146.0
44	28727.0
45	28727.0
46	28021.0
47	27315.0
48	27315.0
49	27467.0
50	27619.0
51	25560.0
52	23501.0
53	23501.0
54	22815.0
55	22129.0
56	22129.0
57	21299.5
58	20470.0
59	19232.5
60	17995.0
61	17995.0
62	16874.5
63	15754.0
64	14241.5
65	12729.0
66	12729.0
67	11312.0
68	9895.0
69	9895.0
70	8178.0
71	6461.0
72	5303.0
73	4145.0
74	4145.0
75	3391.5
76	2638.0
77	2638.0
78	2031.0
79	1424.0
80	1124.5
81	825.0
82	825.0
83	649.5
84	474.0
85	474.0
86	332.5
87	191.0
88	143.0
89	95.0
90	95.0
91	58.5
92	22.0
93	15.0
94	8.0
95	8.0
96	7.0
97	6.0
98	6.0
99	6.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009786222543851291
2	0.0
3	0.0
4	5.150643444132258E-4
5	0.0
6	2.575321722066129E-4
7	7.725965166198388E-4
8	0.002317789549859516
9	0.004378046927512419
10	0.0015451930332396775
11	0.0066958364773719355
12	0.0
13	2.575321722066129E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	5.150643444132258E-4
26	0.0
27	2.575321722066129E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	5.150643444132258E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	388301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.568811947687486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.385601509216556	19.063016983924513
2	15.986510917261256	10.093503136933473
3	7.641709227937983	7.23719044727047
4	4.443611693716678	5.611181677099475
5	2.8180965512849774	4.4481980038971045
6	1.8695621640047269	3.541191382798614
7	1.3386577990339166	2.9581885424004857
8	0.95515679259327	2.4122532132746626
9	0.6867801290868405	1.9512749470095907
>10	3.4591501927490227	19.17239971934113
>50	0.23327431161261175	5.0187231935962835
>100	0.16883840036297423	9.754980667456529
>500	0.005709511123385602	1.3299320350379562
>1k	0.005709511123385602	3.6787490773644302
>5k	0.0016312888923958862	3.729216972595299
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	8306	2.139062222348127	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	6177	1.590776227720248	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3903	1.00514806812241	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2694	0.6937916719246152	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2463	0.6343017401448876	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1534	0.3950543521649442	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	1361	0.35050128637320016	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	1283	0.33041377694108437	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1049	0.27015124864473694	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	951	0.24491309576848888	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	860	0.22147766809768712	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	802	0.20654080210970355	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	716	0.18439303529993484	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	684	0.17615200578932322	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	594	0.15297411029072805	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	558	0.14370295209129	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	491	0.12644829655344694	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	467	0.12026752442048823	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	463	0.11923739573166177	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	454	0.11691960618180226	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	429	0.11048130187663693	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT	421	0.10842104449898403	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	402	0.10352793322705839	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC	400	0.10301286888264516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.575321722066129E-4	0.0
11	0.0	0.0	0.0	2.575321722066129E-4	0.0
12	0.0	0.0	0.0	2.575321722066129E-4	0.0
13	0.0	0.0	0.0	2.575321722066129E-4	0.0
14	0.0	0.0	0.0	2.575321722066129E-4	0.0
15	0.0	0.0	0.0	2.575321722066129E-4	0.0
16	0.0	0.0	0.0	5.150643444132258E-4	0.0
17	0.0	0.0	0.0	5.150643444132258E-4	0.0
18	0.0	0.0	0.0	5.150643444132258E-4	0.0
19	0.0	0.0	0.0	7.725965166198388E-4	0.0
20	0.0	0.0	0.0	0.0010301286888264516	0.0
21	0.0	0.0	0.0	0.002060257377652903	0.0
22	0.0	0.0	0.0	0.0025753217220661293	0.0
23	0.0	0.0	0.0	0.0048931112719256455	0.0
24	0.0	0.0	2.575321722066129E-4	0.007983497338405001	0.0
25	0.0	0.0	2.575321722066129E-4	0.010043754716057904	0.0
26	0.0	0.0	2.575321722066129E-4	0.010301286888264517	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGGGT	35	0.0020629333	22.85935	5
GAAAGCT	35	0.0020644786	22.856407	4
CACACGT	35	0.0020644786	22.856407	18
GGCTTGG	45	3.528642E-4	21.335396	7
GTTATAT	70	7.749877E-7	20.573416	1
GGATAGA	40	0.004475725	20.001932	1
ACGTCAT	40	0.0044790623	19.999357	21
GTAACCA	40	0.0044790623	19.999357	32
GAACTTA	50	7.179535E-4	19.201855	11
AAGACTG	50	7.179535E-4	19.201855	5
TAAGTTC	50	7.18576E-4	19.199383	30
CTTATAG	60	1.13599934E-4	18.666065	2
AACGGGA	80	2.7873775E-6	17.99942	30
GCGAAAC	45	0.0088351	17.779495	11
TAGCCTG	45	0.0088351	17.779495	5
GTTGTGC	45	0.0088351	17.779495	1
TAATACA	45	0.008841658	17.777206	4
ACACGTC	45	0.008841658	17.777206	19
CTCCAGT	45	0.008841658	17.777206	19
ATAGAGG	45	0.008841658	17.777206	3
>>END_MODULE