FastQCFastQC Report
Thu 2 Feb 2017
SRR4062891_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062891_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867908
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27910.32157786309147973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18880.21753457739760435No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16410.18907533978255758No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13330.15358770745286368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10770.12409149356844273No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9220.10623245781810975No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACC555.8394286E-520.3598984
TTGTACT1302.6011548E-1017.2276044
TATTCCC1505.456968E-1217.0615655
CGAGCCG1850.016.433215
AAATCGC908.572135E-616.0007514
TAACGAA1102.0164225E-716.0007513
CCTATTC2500.016.000753
ACGAACG1102.0164225E-716.0007515
AAATTCG600.002441434215.99982721
ACCGTCG1001.3167719E-615.9961398
AGGCCGT600.002446133615.9952176
CGCTTCG1551.891749E-1015.48281132
TGTACTG1551.891749E-1015.4792435
TTAATAC1153.3402102E-715.3050633
CCGATAA1153.3402102E-715.3050639
GTAGGAC1153.3402102E-715.3050633
GTTATTC1153.3402102E-715.3050633
TACCGTC1153.3511424E-715.3006557
ATAGCGT1153.3533433E-715.2997746
AATAGCG1153.3533433E-715.2997745
Produced by FastQC (version 0.11.5)