##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062891_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	867908
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17389400719892	32.0	32.0	32.0	32.0	32.0
2	30.860328514082138	32.0	32.0	32.0	32.0	32.0
3	30.90124644547579	32.0	32.0	32.0	32.0	32.0
4	30.96231167358752	32.0	32.0	32.0	32.0	32.0
5	30.906532720057886	32.0	32.0	32.0	32.0	32.0
6	34.48793074842034	36.0	36.0	36.0	32.0	36.0
7	34.423643980698415	36.0	36.0	36.0	32.0	36.0
8	34.41252183411145	36.0	36.0	36.0	32.0	36.0
9	34.532849103822066	36.0	36.0	36.0	32.0	36.0
10	34.28924033422897	36.0	36.0	36.0	32.0	36.0
11	34.53156325324804	36.0	36.0	36.0	32.0	36.0
12	34.37367094208142	36.0	36.0	36.0	32.0	36.0
13	34.44556911562055	36.0	36.0	36.0	32.0	36.0
14	34.35693184070201	36.0	36.0	36.0	32.0	36.0
15	34.323072261115236	36.0	36.0	36.0	32.0	36.0
16	34.33110997939874	36.0	36.0	36.0	32.0	36.0
17	34.27188019928379	36.0	36.0	36.0	32.0	36.0
18	34.28912972342691	36.0	36.0	36.0	32.0	36.0
19	34.26736935251202	36.0	36.0	36.0	32.0	36.0
20	34.240912631292716	36.0	36.0	36.0	32.0	36.0
21	34.21439830028068	36.0	36.0	36.0	32.0	36.0
22	34.200213617111494	36.0	36.0	36.0	32.0	36.0
23	34.14701558229674	36.0	36.0	36.0	32.0	36.0
24	34.13657553565585	36.0	36.0	36.0	32.0	36.0
25	34.120572687427696	36.0	36.0	36.0	32.0	36.0
26	34.073136784083104	36.0	36.0	36.0	32.0	36.0
27	34.07261944814427	36.0	36.0	36.0	32.0	36.0
28	34.05254128317748	36.0	36.0	36.0	32.0	36.0
29	34.01732211248197	36.0	36.0	36.0	32.0	36.0
30	33.98335537868069	36.0	36.0	36.0	32.0	36.0
31	34.00955170363679	36.0	36.0	36.0	32.0	36.0
32	33.96066749010264	36.0	36.0	36.0	32.0	36.0
33	33.90563055070353	36.0	36.0	36.0	32.0	36.0
34	33.911982606451375	36.0	36.0	36.0	32.0	36.0
35	33.84831456790351	36.0	36.0	36.0	32.0	36.0
36	33.809304672845506	36.0	36.0	36.0	32.0	36.0
37	33.80722150273992	36.0	36.0	36.0	32.0	36.0
38	33.156838051959426	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	3.0
10	2.0
11	5.0
12	2.0
13	6.0
14	138.0
15	278.0
16	368.0
17	485.0
18	571.0
19	776.0
20	1016.0
21	1253.0
22	1766.0
23	2607.0
24	3529.0
25	5447.0
26	7699.0
27	11528.0
28	16075.0
29	22247.0
30	30741.0
31	42483.0
32	58541.0
33	88329.0
34	193684.0
35	378323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.78411394252345	17.716650342688183	11.794795477277368	25.704440237511
2	16.113561584771144	20.417313230482613	38.121018300542076	25.34810688420417
3	18.42438238527543	24.97770156745881	29.0064002839449	27.591515763320867
4	12.420782884033056	16.00450759792964	36.785663257546084	34.789046260491226
5	14.021679767163725	36.935336315223076	34.21626305152862	14.826720866084575
6	32.70741925374235	36.27866676498783	17.64492386254701	13.36899011872281
7	29.0909866022666	31.13463639003212	21.724768062974416	18.04960894472686
8	27.62175035689472	32.93271252663028	20.242699933057036	19.202837183417962
9	27.569607272685374	14.43879226413029	19.11937366416831	38.87222679901602
10	15.455103750312535	26.989944705674958	32.43019653163206	25.124755012380444
11	36.22646031321725	21.73071427913022	23.018426170877618	19.024399236774915
12	24.39246403062136	24.30673897989494	29.334328850051445	21.966468139432255
13	29.269551830153627	19.992947651291317	25.65234225555315	25.085158263001905
14	23.35778114503333	20.097944885836593	26.07350475608843	30.47076921304165
15	24.702289372540207	27.14551604519969	23.492431183409106	24.65976339885099
16	25.06968602972272	26.199412090742214	24.49606543284848	24.234836446686586
17	23.499638717082632	26.08128462294625	26.193184371834455	24.225892288136667
18	24.33829146631915	25.125278412475645	27.104268280738868	23.43216184046634
19	24.970415019375714	25.738184156277832	26.327003050104913	22.964397774241537
20	25.104537907306074	24.482553202276385	26.26174287073941	24.15116601967813
21	26.23542818690176	24.693699538057537	25.138473273822758	23.932399001217945
22	25.025695625521404	24.978567774228786	25.58466190087711	24.411074699372705
23	23.67367915255585	24.864115561404095	26.12150033474182	25.340704951298232
24	24.056170012698868	25.72603636346875	25.912831847189537	24.304961776642845
25	24.523411958980528	24.928582376008748	25.96213569521413	24.585869969796597
26	23.912970827019148	25.577265472397166	26.392242049887354	24.117521650696336
27	24.7018154372663	25.55151612921811	25.57283354554725	24.173834887968333
28	23.941068564370127	25.062683210117807	26.498635718368025	24.497612507144044
29	23.850896893281973	25.80162681818653	26.37489643697088	23.972579851560617
30	24.183419980458307	25.49591652379109	26.43486256291134	23.88580093283926
31	24.313857435366806	25.449641013113883	25.558299216808262	24.678202334711052
32	23.91309608171872	25.666038681576993	25.548161847313207	24.872703389391084
33	23.716808244875757	25.205914481537988	26.17856369175743	24.898713581828822
34	24.090784953832006	25.517020450352558	26.402007815083735	23.990186780731705
35	25.168362196521667	25.2967369308989	25.90415454750787	23.630746325071563
36	24.139536384462566	25.830732851802512	25.75732782661203	24.27240293712289
37	24.813903215844615	25.82170660545638	25.496642191770718	23.867747986928286
38	23.944548084867105	25.81307159869609	25.856742689701345	24.385637626735466
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	348.0
1	299.5
2	251.0
3	251.0
4	607.0
5	963.0
6	963.0
7	1118.5
8	1274.0
9	1196.0
10	1118.0
11	1118.0
12	1407.0
13	1696.0
14	2258.5
15	2821.0
16	2821.0
17	3946.5
18	5072.0
19	5072.0
20	6111.5
21	7151.0
22	7726.5
23	8302.0
24	8302.0
25	9665.0
26	11028.0
27	11028.0
28	13912.5
29	16797.0
30	20111.5
31	23426.0
32	23426.0
33	28540.5
34	33655.0
35	33655.0
36	37144.5
37	40634.0
38	45614.0
39	50594.0
40	50594.0
41	54310.0
42	58026.0
43	64587.5
44	71149.0
45	71149.0
46	74933.5
47	78718.0
48	78718.0
49	80046.0
50	81374.0
51	79575.5
52	77777.0
53	77777.0
54	73068.0
55	68359.0
56	68359.0
57	65258.5
58	62158.0
59	55413.5
60	48669.0
61	48669.0
62	45240.0
63	41811.0
64	34497.0
65	27183.0
66	27183.0
67	22495.0
68	17807.0
69	17807.0
70	14506.5
71	11206.0
72	8907.0
73	6608.0
74	6608.0
75	5056.0
76	3504.0
77	3504.0
78	3326.5
79	3149.0
80	2446.0
81	1743.0
82	1743.0
83	1748.5
84	1754.0
85	1754.0
86	1180.5
87	607.0
88	505.0
89	403.0
90	403.0
91	292.5
92	182.0
93	148.5
94	115.0
95	115.0
96	102.0
97	89.0
98	89.0
99	238.0
100	387.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1250132502523309
2	0.05784023191398167
3	0.015439424455126579
4	0.00460878341944077
5	2.3043917097203854E-4
6	4.6087834194407707E-4
7	0.0
8	5.760979274300963E-4
9	3.456587564580578E-4
10	8.065370984021348E-4
11	0.0013826350258322312
12	0.001958732953262327
13	0.012904593574434157
14	0.008411029740479406
15	0.023389575853661908
16	0.010024103937283676
17	0.019241670776165214
18	0.004724003004926789
19	0.007950151398535329
20	0.004724003004926789
21	0.005876198859786982
22	0.006452296787217079
23	0.008641468911451443
24	0.013135032745406196
25	0.014402448185752406
26	0.017628596579360944
27	0.008411029740479406
28	0.005991418445273002
29	0.009102347253395522
30	0.002304391709720385
31	0.005645759688814944
32	0.0061066380307590205
33	0.008295810154993387
34	0.011406738963115906
35	0.015669863626098617
36	0.013826350258322309
37	0.00921756683888154
38	0.006567516372703098
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	867908.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.27280674502308
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.62676444022821	54.391996763233784
2	10.293467572446405	13.231801040400192
3	2.431331156542763	4.6880543267287935
4	0.9094673458804902	2.3381607585074327
5	0.46150394078153395	1.4831076798959053
6	0.2507613584930826	0.9670281800127211
7	0.16753204776321648	0.7537428450638232
8	0.11551034631424768	0.5939339332565065
9	0.07631271190314383	0.4414348965905448
>10	0.516911493789678	6.45323514818616
>50	0.07800690187377378	3.5166110322914355
>100	0.06703882168929581	8.196834582272063
>500	0.004493218578509805	1.9355979549565072
>1k	8.986437157019611E-4	1.0084608586041006
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2791	0.32157786309147973	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1888	0.21753457739760435	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1641	0.18907533978255758	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1333	0.15358770745286368	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1077	0.12409149356844273	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	922	0.10623245781810975	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.1521958548601925E-4	0.0
4	0.0	0.0	0.0	1.1521958548601925E-4	0.0
5	0.0	0.0	0.0	1.1521958548601925E-4	0.0
6	0.0	0.0	0.0	1.1521958548601925E-4	1.1521958548601925E-4
7	0.0	0.0	0.0	1.1521958548601925E-4	1.1521958548601925E-4
8	0.0	0.0	0.0	1.1521958548601925E-4	1.1521958548601925E-4
9	0.0	0.0	0.0	1.1521958548601925E-4	1.1521958548601925E-4
10	0.0	0.0	0.0	1.1521958548601925E-4	1.1521958548601925E-4
11	0.0	0.0	0.0	1.1521958548601925E-4	1.1521958548601925E-4
12	0.0	0.0	0.0	1.1521958548601925E-4	2.304391709720385E-4
13	0.0	0.0	0.0	1.1521958548601925E-4	2.304391709720385E-4
14	0.0	0.0	0.0	1.1521958548601925E-4	2.304391709720385E-4
15	0.0	0.0	0.0	1.1521958548601925E-4	2.304391709720385E-4
16	0.0	0.0	0.0	1.1521958548601925E-4	2.304391709720385E-4
17	0.0	0.0	0.0	1.1521958548601925E-4	2.304391709720385E-4
18	0.0	0.0	0.0	1.1521958548601925E-4	2.304391709720385E-4
19	0.0	0.0	0.0	1.1521958548601925E-4	2.304391709720385E-4
20	0.0	0.0	0.0	2.304391709720385E-4	3.4565875645805775E-4
21	0.0	0.0	0.0	3.4565875645805775E-4	4.60878341944077E-4
22	1.1521958548601925E-4	0.0	0.0	3.4565875645805775E-4	4.60878341944077E-4
23	1.1521958548601925E-4	1.1521958548601925E-4	0.0	0.0011521958548601926	6.913175129161155E-4
24	1.1521958548601925E-4	1.1521958548601925E-4	0.0	0.0019587329532623275	6.913175129161155E-4
25	1.1521958548601925E-4	1.1521958548601925E-4	0.0	0.0024196112952064044	6.913175129161155E-4
26	1.1521958548601925E-4	1.1521958548601925E-4	0.0	0.0032261483936085393	6.913175129161155E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACC	55	5.8394286E-5	20.359898	4
TTGTACT	130	2.6011548E-10	17.227604	4
TATTCCC	150	5.456968E-12	17.061565	5
CGAGCCG	185	0.0	16.4332	15
AAATCGC	90	8.572135E-6	16.00075	14
TAACGAA	110	2.0164225E-7	16.00075	13
CCTATTC	250	0.0	16.00075	3
ACGAACG	110	2.0164225E-7	16.00075	15
AAATTCG	60	0.0024414342	15.999827	21
ACCGTCG	100	1.3167719E-6	15.996139	8
AGGCCGT	60	0.0024461336	15.995217	6
CGCTTCG	155	1.891749E-10	15.482811	32
TGTACTG	155	1.891749E-10	15.479243	5
TTAATAC	115	3.3402102E-7	15.305063	3
CCGATAA	115	3.3402102E-7	15.305063	9
GTAGGAC	115	3.3402102E-7	15.305063	3
GTTATTC	115	3.3402102E-7	15.305063	3
TACCGTC	115	3.3511424E-7	15.300655	7
ATAGCGT	115	3.3533433E-7	15.299774	6
AATAGCG	115	3.3533433E-7	15.299774	5
>>END_MODULE