FastQCFastQC Report
Thu 2 Feb 2017
SRR4062891_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062891_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867908
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31550.36351779220839076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22760.2622397765661798No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16940.19518197781331662No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC16090.18538831304700498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12740.14678975190918853No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9660.11130211957949461No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8800.10139323522769694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATAA502.7932443E-522.403471
ATCGTTT1650.018.4239129
CGTTTAT1700.017.88203231
TATACTG1101.0615622E-817.4552365
TCTAGCG1700.016.94087228
CTAGCGG1750.016.45684629
CATCGTT1850.016.43213728
GTATATA1501.1459633E-1016.0024781
TGTACCG600.002440614216.0006335
TCAATAC703.6956012E-415.9997123
CTAATGT600.00244155315.9997114
CGGCATC1950.015.58946125
CGTCTTA1153.342684E-715.30407215
CGAGATT1052.1837386E-615.2378224
TCGTTTA2000.015.19972730
AGCGGCG2000.015.19972731
TACGCTA2250.014.9339259
ACGCTAT2250.014.93392510
GAGTATA650.004156206314.7715191
GTCTACT650.004156206314.7715191
Produced by FastQC (version 0.11.5)