##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062891_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	867908
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.084689851919787	32.0	32.0	32.0	32.0	32.0
2	31.103693018153997	32.0	32.0	32.0	32.0	32.0
3	31.252841314978085	32.0	32.0	32.0	32.0	32.0
4	31.379912387027197	32.0	32.0	32.0	32.0	32.0
5	31.261021905547594	32.0	32.0	32.0	32.0	32.0
6	34.789739235034126	36.0	36.0	36.0	32.0	36.0
7	34.817146517833685	36.0	36.0	36.0	32.0	36.0
8	34.75310977661227	36.0	36.0	36.0	32.0	36.0
9	34.90665830940607	36.0	36.0	36.0	32.0	36.0
10	34.69855560727635	36.0	36.0	36.0	32.0	36.0
11	34.925981786087924	36.0	36.0	36.0	36.0	36.0
12	34.804589887407424	36.0	36.0	36.0	32.0	36.0
13	34.861257183941156	36.0	36.0	36.0	32.0	36.0
14	34.815111739954006	36.0	36.0	36.0	32.0	36.0
15	34.76488521824894	36.0	36.0	36.0	32.0	36.0
16	34.78576415933486	36.0	36.0	36.0	32.0	36.0
17	34.74764606386852	36.0	36.0	36.0	32.0	36.0
18	34.74911280919176	36.0	36.0	36.0	32.0	36.0
19	34.742862146679144	36.0	36.0	36.0	32.0	36.0
20	34.72618411168004	36.0	36.0	36.0	32.0	36.0
21	34.708393055485146	36.0	36.0	36.0	32.0	36.0
22	34.69634223903916	36.0	36.0	36.0	32.0	36.0
23	34.63962770247538	36.0	36.0	36.0	32.0	36.0
24	34.613097240721366	36.0	36.0	36.0	32.0	36.0
25	34.602250468943716	36.0	36.0	36.0	32.0	36.0
26	34.53086732695171	36.0	36.0	36.0	32.0	36.0
27	34.50575060951161	36.0	36.0	36.0	32.0	36.0
28	34.47182650695696	36.0	36.0	36.0	32.0	36.0
29	34.436159132073904	36.0	36.0	36.0	32.0	36.0
30	34.40816999036764	36.0	36.0	36.0	32.0	36.0
31	34.40003087884891	36.0	36.0	36.0	32.0	36.0
32	34.37298423335192	36.0	36.0	36.0	32.0	36.0
33	34.32494227498767	36.0	36.0	36.0	32.0	36.0
34	34.30930006406209	36.0	36.0	36.0	32.0	36.0
35	34.27720334413325	36.0	36.0	36.0	32.0	36.0
36	34.23419187287132	36.0	36.0	36.0	32.0	36.0
37	34.22398111320555	36.0	36.0	36.0	32.0	36.0
38	33.760902077178685	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	5.0
21	14.0
22	63.0
23	201.0
24	566.0
25	1482.0
26	3115.0
27	6213.0
28	10858.0
29	17390.0
30	26674.0
31	39739.0
32	58271.0
33	92456.0
34	212365.0
35	398494.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.04861939313908	19.17455115729458	12.583382017254275	26.193447432312066
2	15.188937076279974	21.67372578660411	37.16396207893003	25.973375058185887
3	17.35967406683658	25.84870746669002	29.386063960696298	27.405554505777108
4	11.884955018066515	17.131387803259347	36.93254092618539	34.051116252488754
5	13.637505357710724	37.576217755799	33.667623757356765	15.118653129133502
6	32.6921348625312	36.899042062158806	17.143446410095102	13.265376665214895
7	28.78361391255054	31.51720172783489	21.631926001288157	18.06725835832641
8	27.40890870187915	33.345239562941515	20.02159252350232	19.224259211677015
9	27.408438435462724	14.371703196041876	18.825283504976458	39.39457486351895
10	15.323955865679759	27.263059168379623	32.371274899296694	25.04171006664393
11	36.411413401055704	21.89473611435985	22.654423482209626	19.039427002374815
12	24.20979254641925	24.53932169995564	29.330744724364994	21.920141029260115
13	29.38264122768914	20.143632900760107	25.499736722943815	24.973989148606936
14	23.447070426819433	20.07804974720823	25.86806435705167	30.60681546892067
15	24.835235992754992	27.251275480811334	23.20925720237629	24.70423132405739
16	24.9241855127502	26.39749835235993	24.437497983657256	24.240818151232617
17	23.511132516349658	26.13099545113077	25.993077607304	24.364794425215575
18	24.35108329454274	25.196795052010124	27.00136419989215	23.450757453554985
19	24.989169358964315	25.740746715089617	26.212686137240354	23.057397788705718
20	25.139070039681627	24.542923904376963	26.242067131539287	24.075938924402127
21	26.281126571019048	24.819911787885353	25.091369131290413	23.807592509805186
22	24.999769560829026	25.030302750982823	25.538997220903596	24.43093046728455
23	23.761038087161715	24.735108952142173	26.139411732173144	25.36444122852297
24	24.065453942122897	25.836609410213985	25.78671932969854	24.311217317964577
25	24.440608912465375	24.928563856998668	25.928554639431827	24.702272591104126
26	24.05439286191624	25.495444217589885	26.329864455679637	24.120298464814244
27	24.876599823944474	25.45961092650373	25.447973748369645	24.215815501182153
28	23.891933246380955	25.060375062794677	26.46225175940307	24.585439931421302
29	24.020633523368836	25.56261723592823	26.349912663554203	24.06683657714873
30	24.317093516824364	25.358448130447	26.45107545961093	23.873382893117704
31	24.422058559202128	25.31789083635593	25.537153707535822	24.722896896906125
32	24.038607778704655	25.617461758619577	25.627370642971375	24.716559819704393
33	23.842964922549395	25.11049558248109	26.10737543610613	24.93916405886338
34	24.28563856998668	25.344967438945144	26.386206832982296	23.98318715808588
35	25.219493310350867	25.175940307037152	25.82543311042184	23.77913327219014
36	24.22019384543062	25.674149794678698	25.699498103485624	24.406158256405057
37	25.01186761730506	25.61354429271305	25.48599621158003	23.888591878401858
38	24.190236753204257	25.414905727335157	25.921065366375238	24.47379215308535
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	225.0
1	191.0
2	157.0
3	157.0
4	442.5
5	728.0
6	728.0
7	937.5
8	1147.0
9	1144.5
10	1142.0
11	1142.0
12	1432.5
13	1723.0
14	2217.0
15	2711.0
16	2711.0
17	4078.5
18	5446.0
19	5446.0
20	6401.5
21	7357.0
22	7933.5
23	8510.0
24	8510.0
25	9881.0
26	11252.0
27	11252.0
28	14314.5
29	17377.0
30	20434.0
31	23491.0
32	23491.0
33	28600.5
34	33710.0
35	33710.0
36	37086.5
37	40463.0
38	45397.5
39	50332.0
40	50332.0
41	54065.5
42	57799.0
43	63848.5
44	69898.0
45	69898.0
46	74725.5
47	79553.0
48	79553.0
49	80967.0
50	82381.0
51	79913.5
52	77446.0
53	77446.0
54	72795.0
55	68144.0
56	68144.0
57	65492.0
58	62840.0
59	55931.0
60	49022.0
61	49022.0
62	45520.5
63	42019.0
64	34571.5
65	27124.0
66	27124.0
67	22640.0
68	18156.0
69	18156.0
70	14542.5
71	10929.0
72	8747.5
73	6566.0
74	6566.0
75	4924.5
76	3283.0
77	3283.0
78	3118.0
79	2953.0
80	2307.5
81	1662.0
82	1662.0
83	1590.0
84	1518.0
85	1518.0
86	990.5
87	463.0
88	370.0
89	277.0
90	277.0
91	163.0
92	49.0
93	41.0
94	33.0
95	33.0
96	21.5
97	10.0
98	10.0
99	11.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007489273056591252
2	0.0
3	0.0
4	4.6087834194407707E-4
5	0.0
6	2.3043917097203854E-4
7	1.1521958548601927E-4
8	0.0017282937822902886
9	0.0025348308806924236
10	0.0013826350258322312
11	0.005876198859786982
12	3.456587564580578E-4
13	1.1521958548601927E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	4.6087834194407707E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	867908.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.559598302805405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.53555853002985	52.25950985639156
2	10.99742723723705	13.759892606517617
3	2.6244275812400666	4.9254940577154525
4	0.9626273684292164	2.4088632593687374
5	0.49183163558129256	1.538439477728872
6	0.27576720245245806	1.0351131246308514
7	0.18980624773985239	0.8311941829777552
8	0.12501980232666127	0.6256950890761652
9	0.08427468631882679	0.47449714708806606
>10	0.5581102456165834	6.777206252901466
>50	0.07748452739951586	3.4298436170811644
>100	0.07213061719812897	8.777027943641382
>500	0.004611932025482199	2.0022176592572642
>1k	9.223864050964397E-4	1.155005725623765
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3155	0.36351779220839076	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2276	0.2622397765661798	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1694	0.19518197781331662	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1609	0.18538831304700498	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1274	0.14678975190918853	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	966	0.11130211957949461	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	880	0.10139323522769694	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.1521958548601925E-4	0.0
4	0.0	0.0	0.0	1.1521958548601925E-4	0.0
5	0.0	0.0	0.0	1.1521958548601925E-4	0.0
6	0.0	0.0	0.0	1.1521958548601925E-4	1.1521958548601925E-4
7	0.0	0.0	0.0	1.1521958548601925E-4	1.1521958548601925E-4
8	0.0	0.0	0.0	1.1521958548601925E-4	1.1521958548601925E-4
9	0.0	0.0	0.0	1.1521958548601925E-4	1.1521958548601925E-4
10	0.0	0.0	0.0	1.1521958548601925E-4	1.1521958548601925E-4
11	0.0	0.0	0.0	1.1521958548601925E-4	1.1521958548601925E-4
12	0.0	0.0	0.0	1.1521958548601925E-4	1.1521958548601925E-4
13	0.0	0.0	0.0	1.1521958548601925E-4	2.304391709720385E-4
14	0.0	0.0	0.0	1.1521958548601925E-4	2.304391709720385E-4
15	0.0	0.0	0.0	1.1521958548601925E-4	2.304391709720385E-4
16	0.0	0.0	0.0	1.1521958548601925E-4	2.304391709720385E-4
17	0.0	0.0	0.0	1.1521958548601925E-4	2.304391709720385E-4
18	0.0	0.0	0.0	1.1521958548601925E-4	2.304391709720385E-4
19	0.0	0.0	0.0	1.1521958548601925E-4	2.304391709720385E-4
20	0.0	0.0	0.0	1.1521958548601925E-4	2.304391709720385E-4
21	0.0	0.0	0.0	2.304391709720385E-4	2.304391709720385E-4
22	0.0	0.0	0.0	2.304391709720385E-4	2.304391709720385E-4
23	0.0	0.0	0.0	8.065370984021348E-4	3.4565875645805775E-4
24	0.0	0.0	0.0	0.0017282937822902889	3.4565875645805775E-4
25	0.0	0.0	0.0	0.0024196112952064044	3.4565875645805775E-4
26	0.0	0.0	0.0	0.0032261483936085393	4.60878341944077E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCATAA	50	2.7932443E-5	22.40347	1
ATCGTTT	165	0.0	18.42391	29
CGTTTAT	170	0.0	17.882032	31
TATACTG	110	1.0615622E-8	17.455236	5
TCTAGCG	170	0.0	16.940872	28
CTAGCGG	175	0.0	16.456846	29
CATCGTT	185	0.0	16.432137	28
GTATATA	150	1.1459633E-10	16.002478	1
TGTACCG	60	0.0024406142	16.000633	5
TCAATAC	70	3.6956012E-4	15.999712	3
CTAATGT	60	0.002441553	15.999711	4
CGGCATC	195	0.0	15.589461	25
CGTCTTA	115	3.342684E-7	15.304072	15
CGAGATT	105	2.1837386E-6	15.237822	4
TCGTTTA	200	0.0	15.199727	30
AGCGGCG	200	0.0	15.199727	31
TACGCTA	225	0.0	14.933925	9
ACGCTAT	225	0.0	14.933925	10
GAGTATA	65	0.0041562063	14.771519	1
GTCTACT	65	0.0041562063	14.771519	1
>>END_MODULE