FastQCFastQC Report
Thu 2 Feb 2017
SRR4062890_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062890_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363588
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27990.7698273870424767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19330.5316457088792809No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15810.434832832766758No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13180.3624982122622309No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10050.2764117627644477No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.23845671474306082No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7420.20407714225992057No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT6430.1768485208532735No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG6420.17657348427340835No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG6350.1746482282143525No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA5690.15649581394325446No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG5650.15539566762379395No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC5630.1548455944640637No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC5380.14796967996743565No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG5240.14411916784932396No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC5240.14411916784932396No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG5240.14411916784932396No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4760.1309174120157981No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4670.128442082797012No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4560.12541668041849566No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT4260.117165583022542No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA4240.11661550986281174No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG4220.11606543670308152No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA4210.11579040012321638No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA4170.11469025380375589No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT4170.11469025380375589No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG4030.11083974168564419No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC4010.11028966852591394No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT3860.10616411982793711No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.10451390034874639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCCA509.640298E-725.601659
TTAGGAC701.1150405E-925.141023
CTAAGAC350.00206471722.8554733
GTCCTAG453.5059077E-421.3552741
TTGATTA604.7088943E-621.33177625
ATACCGT851.053013E-820.7015236
TCGCGTA555.81636E-520.364959
GCGTAAC555.81636E-520.3649511
GTCGCGT555.822524E-520.3621488
CATCGTT555.822524E-520.36214828
GTTAGGA400.00446889220.0067942
CCGTCGT801.2586861E-720.001299
CGTCGTA801.2586861E-720.0012910
TATGCTG400.0044831519.9957895
ATACTCT400.0044831519.9957896
ACCGTCG902.028537E-819.5541278
TACCGTC902.028537E-819.5541277
AGCGTAT751.5038313E-619.1985978
TAGCGTA751.5038313E-619.1985977
AATAGCG751.505814E-619.1959575
Produced by FastQC (version 0.11.5)