##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062890_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	363588
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.05530435547928	32.0	32.0	32.0	32.0	32.0
2	30.717559435404908	32.0	32.0	32.0	32.0	32.0
3	30.79696524637777	32.0	32.0	32.0	32.0	32.0
4	30.818915915816802	32.0	32.0	32.0	32.0	32.0
5	30.786112302936292	32.0	32.0	32.0	32.0	32.0
6	34.400134217850976	36.0	36.0	36.0	32.0	36.0
7	34.274747791456264	36.0	36.0	36.0	32.0	36.0
8	34.23037889039242	36.0	36.0	36.0	32.0	36.0
9	34.32855869830688	36.0	36.0	36.0	32.0	36.0
10	34.0956302188191	36.0	36.0	36.0	32.0	36.0
11	34.40262054853296	36.0	36.0	36.0	32.0	36.0
12	34.20282572582153	36.0	36.0	36.0	32.0	36.0
13	34.28774326985489	36.0	36.0	36.0	32.0	36.0
14	34.184178245706676	36.0	36.0	36.0	32.0	36.0
15	34.14265872361024	36.0	36.0	36.0	32.0	36.0
16	34.14848674873758	36.0	36.0	36.0	32.0	36.0
17	34.05309581174296	36.0	36.0	36.0	32.0	36.0
18	34.09643882636391	36.0	36.0	36.0	32.0	36.0
19	34.07090993102083	36.0	36.0	36.0	32.0	36.0
20	34.03379099420223	36.0	36.0	36.0	32.0	36.0
21	33.97856364896531	36.0	36.0	36.0	32.0	36.0
22	33.967204638216884	36.0	36.0	36.0	32.0	36.0
23	33.91474691133921	36.0	36.0	36.0	32.0	36.0
24	33.89797793106483	36.0	36.0	36.0	32.0	36.0
25	33.86019340572296	36.0	36.0	36.0	32.0	36.0
26	33.82419936851601	36.0	36.0	36.0	32.0	36.0
27	33.83498905354412	36.0	36.0	36.0	32.0	36.0
28	33.795755085426364	36.0	36.0	36.0	32.0	36.0
29	33.77272902296006	36.0	36.0	36.0	32.0	36.0
30	33.71684709066306	36.0	36.0	36.0	27.0	36.0
31	33.754898401487395	36.0	36.0	36.0	27.0	36.0
32	33.672467188136025	36.0	36.0	36.0	27.0	36.0
33	33.6003223428716	36.0	36.0	36.0	27.0	36.0
34	33.5854401135351	36.0	36.0	36.0	27.0	36.0
35	33.50373224638877	36.0	36.0	36.0	21.0	36.0
36	33.437286159059155	36.0	36.0	36.0	21.0	36.0
37	33.432924078902495	36.0	36.0	36.0	21.0	36.0
38	32.790749419672814	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	1.0
11	2.0
12	1.0
13	0.0
14	105.0
15	207.0
16	237.0
17	252.0
18	333.0
19	425.0
20	568.0
21	745.0
22	1009.0
23	1448.0
24	2060.0
25	2906.0
26	4156.0
27	5906.0
28	8039.0
29	11050.0
30	14322.0
31	19079.0
32	25168.0
33	36716.0
34	77794.0
35	151055.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.03912583715192	16.85318998942545	11.41941311244272	24.688271060979908
2	16.260804715685655	20.618190724387215	37.28710479026262	25.833899769664516
3	18.992185157575943	25.814286775284085	28.341113333094935	26.852414734045038
4	12.252907896220629	15.370799743659564	35.45776846297504	36.91852389714477
5	14.17954987389538	37.441382667697134	32.423876541240475	15.955190917167005
6	33.11376125593395	35.38557590226246	17.39258387286639	14.1080789689372
7	29.392334180446	31.370672299415826	20.77241273089321	18.46458078924497
8	29.206214743651447	31.29017264093601	19.904452029362986	19.599160586049553
9	28.477522450046067	13.864708390060096	18.706492292036252	38.95127686785759
10	16.68358155589477	26.40868022608193	30.43139843502895	26.476339782994344
11	37.51265182186235	21.515248195740185	21.506721967963387	19.46537801443408
12	25.35824105703803	24.512764658316417	28.108652243510406	22.020342041135148
13	30.297387032263924	19.357802455347933	25.348730376609552	24.996080135778595
14	24.844592364396522	19.904004841016615	24.972769281549127	30.27863351303774
15	25.58438950313481	26.224831430072708	24.250410592601355	23.940368474191125
16	25.448083884383905	25.583141703433938	24.496627680526366	24.47214673165579
17	23.374808465218383	25.366220004346495	26.10567493143261	25.153296599002505
18	25.05789742940914	23.55999053837733	28.023731069878483	23.35838096233504
19	25.06360588282215	25.325180778233445	26.48067618898305	23.130537149961356
20	25.693602110167696	22.96182609819761	26.885934874151822	24.45863691748287
21	27.53357702981844	23.778835812931316	24.548982718453544	24.1386044387967
22	25.516277739270116	24.186388091230153	25.589442299017506	24.707891870482225
23	23.719107150224865	23.89184580049786	26.289007165353244	26.100039883924026
24	24.06378425364061	25.859469986631385	25.91283442171107	24.163911338016934
25	24.87834399284781	24.029709098411388	25.98583316140568	25.106113747335122
26	23.59648543667954	25.398056756786495	27.043651448629497	23.961806357904468
27	25.068283277267238	24.89389617641153	25.899235062259496	24.138585484061732
28	23.908606823407744	24.39791843156239	26.931941953725808	24.76153279130406
29	23.257285265526402	25.161879507732255	27.37867976739964	24.202155459341697
30	23.9903184993674	25.028054348424007	27.32521040761318	23.65641674459541
31	23.970349588799955	25.314244849685068	25.647055587644747	25.06834997387023
32	23.30534231466379	25.650701792507064	25.62347174814134	25.420484144687805
33	23.27094192235226	25.008457909864813	26.372175539449614	25.348424628333312
34	24.13354898336414	25.307796408766837	26.867683302526185	23.69097130534284
35	25.449889143793982	25.020493268855184	26.392854588566685	23.136762998784157
36	23.82673565551787	26.369805549366365	25.5382533277218	24.265205467393965
37	24.901391249814335	26.169414508826648	25.84346926762717	23.08572497373184
38	23.960304537253005	25.910421159177925	25.677177309334155	24.452096994234914
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	416.0
1	321.0
2	226.0
3	226.0
4	549.5
5	873.0
6	873.0
7	1007.5
8	1142.0
9	1076.0
10	1010.0
11	1010.0
12	1226.5
13	1443.0
14	1670.5
15	1898.0
16	1898.0
17	2738.5
18	3579.0
19	3579.0
20	3996.5
21	4414.0
22	3888.5
23	3363.0
24	3363.0
25	3560.5
26	3758.0
27	3758.0
28	4845.5
29	5933.0
30	7258.0
31	8583.0
32	8583.0
33	10291.5
34	12000.0
35	12000.0
36	12854.5
37	13709.0
38	16111.5
39	18514.0
40	18514.0
41	19611.0
42	20708.0
43	23822.5
44	26937.0
45	26937.0
46	30196.5
47	33456.0
48	33456.0
49	33410.0
50	33364.0
51	32575.0
52	31786.0
53	31786.0
54	29644.0
55	27502.0
56	27502.0
57	27466.0
58	27430.0
59	24599.5
60	21769.0
61	21769.0
62	21379.0
63	20989.0
64	17265.0
65	13541.0
66	13541.0
67	11422.5
68	9304.0
69	9304.0
70	7583.5
71	5863.0
72	4651.0
73	3439.0
74	3439.0
75	2631.0
76	1823.0
77	1823.0
78	1773.0
79	1723.0
80	1364.5
81	1006.0
82	1006.0
83	1028.5
84	1051.0
85	1051.0
86	696.0
87	341.0
88	306.0
89	271.0
90	271.0
91	188.5
92	106.0
93	88.5
94	71.0
95	71.0
96	70.5
97	70.0
98	70.0
99	123.5
100	177.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12431653409903518
2	0.05638249887235002
3	0.013476792413390979
4	0.0024753292187860987
5	2.7503657986512205E-4
6	5.500731597302441E-4
7	0.0
8	2.7503657986512205E-4
9	8.251097395953663E-4
10	8.251097395953663E-4
11	0.0011001463194604882
12	0.0016502194791907325
13	0.015127011892581714
14	0.0077010242362234175
15	0.024478255607995863
16	0.011001463194604882
17	0.0211778166496144
18	0.0038505121181117087
19	0.005775768177167563
20	0.0041255486979768306
21	0.005775768177167563
22	0.00660087791676293
23	0.009076207135549028
24	0.013751828993256103
25	0.01732730453150269
26	0.01980263375028879
27	0.00797606081608854
28	0.004400585277841953
29	0.012651682673795614
30	0.0022002926389209764
31	0.004950658437572197
32	0.00522569501743732
33	0.006325841336897807
34	0.009901316875144395
35	0.014851975312716592
36	0.014026865573121225
37	0.008251097395953661
38	0.005500731597302441
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	363588.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.84200475749793
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.50093949878477	43.595150374928345
2	10.853702025729339	11.470627481601094
3	2.8183644933503014	4.4678408989793965
4	1.1097127281772055	2.345577810471836
5	0.5785985567744097	1.52871538448774
6	0.353274023377412	1.1200624574405784
7	0.2531760079061114	0.9364829469981351
8	0.16866827197732095	0.7130215704211639
9	0.13304523214961406	0.6327339111191089
>10	0.934591244573489	10.132446241979657
>50	0.1576194550165823	5.891475488812032
>100	0.1294358438546767	12.760910535873634
>500	0.006263024702645649	2.0232124419963036
>1k	0.0026095936261023535	2.3817424548909596
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2799	0.7698273870424767	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1933	0.5316457088792809	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1581	0.434832832766758	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1318	0.3624982122622309	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1005	0.2764117627644477	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	867	0.23845671474306082	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	742	0.20407714225992057	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	643	0.1768485208532735	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	642	0.17657348427340835	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	635	0.1746482282143525	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	569	0.15649581394325446	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	565	0.15539566762379395	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	563	0.1548455944640637	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	538	0.14796967996743565	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	524	0.14411916784932396	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	524	0.14411916784932396	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	524	0.14411916784932396	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	476	0.1309174120157981	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	467	0.128442082797012	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	456	0.12541668041849566	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	426	0.117165583022542	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	424	0.11661550986281174	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	422	0.11606543670308152	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	421	0.11579040012321638	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	417	0.11469025380375589	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	417	0.11469025380375589	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	403	0.11083974168564419	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	401	0.11028966852591394	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	386	0.10616411982793711	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	380	0.10451390034874639	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	2.7503657986512205E-4	0.0	0.0
11	0.0	0.0	2.7503657986512205E-4	0.0	0.0
12	0.0	0.0	2.7503657986512205E-4	0.0	2.7503657986512205E-4
13	0.0	0.0	2.7503657986512205E-4	2.7503657986512205E-4	2.7503657986512205E-4
14	0.0	0.0	2.7503657986512205E-4	2.7503657986512205E-4	2.7503657986512205E-4
15	0.0	0.0	2.7503657986512205E-4	5.500731597302441E-4	2.7503657986512205E-4
16	0.0	0.0	2.7503657986512205E-4	8.251097395953662E-4	2.7503657986512205E-4
17	0.0	0.0	2.7503657986512205E-4	8.251097395953662E-4	2.7503657986512205E-4
18	0.0	0.0	2.7503657986512205E-4	0.0016502194791907323	2.7503657986512205E-4
19	0.0	0.0	2.7503657986512205E-4	0.0016502194791907323	2.7503657986512205E-4
20	0.0	0.0	2.7503657986512205E-4	0.0019252560590558544	5.500731597302441E-4
21	0.0	0.0	2.7503657986512205E-4	0.0033004389583814646	5.500731597302441E-4
22	0.0	0.0	2.7503657986512205E-4	0.0038505121181117087	5.500731597302441E-4
23	0.0	0.0	2.7503657986512205E-4	0.007150951076493174	5.500731597302441E-4
24	0.0	0.0	2.7503657986512205E-4	0.009901316875144395	5.500731597302441E-4
25	0.0	0.0	2.7503657986512205E-4	0.012926719253660736	5.500731597302441E-4
26	0.0	0.0	2.7503657986512205E-4	0.017052267951637567	5.500731597302441E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCATCCA	50	9.640298E-7	25.60165	9
TTAGGAC	70	1.1150405E-9	25.14102	3
CTAAGAC	35	0.002064717	22.855473	3
GTCCTAG	45	3.5059077E-4	21.355274	1
TTGATTA	60	4.7088943E-6	21.331776	25
ATACCGT	85	1.053013E-8	20.701523	6
TCGCGTA	55	5.81636E-5	20.36495	9
GCGTAAC	55	5.81636E-5	20.36495	11
GTCGCGT	55	5.822524E-5	20.362148	8
CATCGTT	55	5.822524E-5	20.362148	28
GTTAGGA	40	0.004468892	20.006794	2
CCGTCGT	80	1.2586861E-7	20.00129	9
CGTCGTA	80	1.2586861E-7	20.00129	10
TATGCTG	40	0.00448315	19.995789	5
ATACTCT	40	0.00448315	19.995789	6
ACCGTCG	90	2.028537E-8	19.554127	8
TACCGTC	90	2.028537E-8	19.554127	7
AGCGTAT	75	1.5038313E-6	19.198597	8
TAGCGTA	75	1.5038313E-6	19.198597	7
AATAGCG	75	1.505814E-6	19.195957	5
>>END_MODULE