FastQCFastQC Report
Thu 2 Feb 2017
SRR4062890_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062890_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363588
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31010.8528884341617434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23040.6336842800092413No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16970.4667370760311121No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14750.405678955301055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14250.3919271263077989No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10100.27778694566377327No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7000.19252560590558543No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG6630.18234925245057593No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC6490.1784987403324642No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG6380.17547333795394787No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT6160.1694225331969152No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC6070.1669472039781291No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG5750.15814603342244518No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC5500.15127011892581713No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG5230.14384413126945886No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5180.14246894837013324No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5140.14136880205067273No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA5140.14136880205067273No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4910.13504296071377495No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA4700.12926719253660737No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC4690.12899215595674224No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.12404149751917004No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG4500.12376646093930493No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT4500.12376646093930493No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG4460.12266631461984444No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4330.11909083908159786No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA4310.1185407659218676No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG4270.1174406196024071No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG4260.117165583022542No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4200.11551536354335126No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG4200.11551536354335126No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT4180.11496529038362102No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA4100.11276499774470004No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT4030.11083974168564419No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC3930.10808937588699297No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC3930.10808937588699297No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT3890.10698922956753247No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT3650.10038835165076955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTC752.4792826E-923.465863
GTAATAT350.002064253422.8563563
CGTCTTA453.5311692E-421.33259815
TGTAATA453.5311692E-421.3325982
CGTTATT851.0510121E-820.7051722
TTCTCGT555.8146303E-520.36573811
ACCTTGT400.00447501520.0020621
TAGGGTC507.1846606E-419.199344
GTCTTAT507.1846606E-419.1993416
CCATAGG601.1345675E-418.6685921
ATACCGT1208.185452E-1118.6685926
TAATACT601.13576534E-418.6660254
CAGATAC1208.185452E-1118.6660253
TATTCCC1055.9299055E-918.28765
CAAGACG1850.018.161544
TACCGTC1159.331416E-1018.0888237
GTGTATA802.7827846E-618.0018561
CCCTAGA450.00883371517.7796121
AACCATC450.00883371517.7796127
TAGGCTG450.00883371517.7796125
Produced by FastQC (version 0.11.5)