##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062890_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	363588
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.052215694687394	32.0	32.0	32.0	32.0	32.0
2	31.324270878026777	32.0	32.0	32.0	32.0	32.0
3	31.393511337007823	32.0	32.0	32.0	32.0	32.0
4	31.501828993256105	32.0	32.0	32.0	32.0	32.0
5	31.421567818519865	32.0	32.0	32.0	32.0	32.0
6	34.951780586818046	36.0	36.0	36.0	36.0	36.0
7	34.97646511986094	36.0	36.0	36.0	36.0	36.0
8	34.92724732389408	36.0	36.0	36.0	36.0	36.0
9	35.00832260690672	36.0	36.0	36.0	36.0	36.0
10	34.87209698889952	36.0	36.0	36.0	32.0	36.0
11	35.041076713202855	36.0	36.0	36.0	36.0	36.0
12	34.934821831303566	36.0	36.0	36.0	32.0	36.0
13	34.95862624729089	36.0	36.0	36.0	36.0	36.0
14	34.906245530655575	36.0	36.0	36.0	32.0	36.0
15	34.890857234012124	36.0	36.0	36.0	32.0	36.0
16	34.88236410442589	36.0	36.0	36.0	32.0	36.0
17	34.84471709737395	36.0	36.0	36.0	32.0	36.0
18	34.844260536651376	36.0	36.0	36.0	32.0	36.0
19	34.81113788133822	36.0	36.0	36.0	32.0	36.0
20	34.79059539918809	36.0	36.0	36.0	32.0	36.0
21	34.75684566047284	36.0	36.0	36.0	32.0	36.0
22	34.75238731751323	36.0	36.0	36.0	32.0	36.0
23	34.684695314476826	36.0	36.0	36.0	32.0	36.0
24	34.65329713851942	36.0	36.0	36.0	32.0	36.0
25	34.63209456857762	36.0	36.0	36.0	32.0	36.0
26	34.56619030331034	36.0	36.0	36.0	32.0	36.0
27	34.55163811786968	36.0	36.0	36.0	32.0	36.0
28	34.49247499917489	36.0	36.0	36.0	32.0	36.0
29	34.451579810114744	36.0	36.0	36.0	32.0	36.0
30	34.41066811885981	36.0	36.0	36.0	32.0	36.0
31	34.38369253110664	36.0	36.0	36.0	32.0	36.0
32	34.35356502414821	36.0	36.0	36.0	32.0	36.0
33	34.26496198994466	36.0	36.0	36.0	32.0	36.0
34	34.2269629360705	36.0	36.0	36.0	32.0	36.0
35	34.15315962022949	36.0	36.0	36.0	32.0	36.0
36	34.144424458452974	36.0	36.0	36.0	32.0	36.0
37	34.08919161248446	36.0	36.0	36.0	32.0	36.0
38	33.63359351793788	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	6.0
22	34.0
23	108.0
24	247.0
25	664.0
26	1357.0
27	2756.0
28	4713.0
29	7514.0
30	11142.0
31	15908.0
32	22106.0
33	34576.0
34	81038.0
35	181413.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.257921128418694	17.959967322508685	12.285141368658765	25.496970180413857
2	15.507662519115042	21.5752445075195	36.37001221162415	26.54708076174131
3	18.18101807540403	26.17440619602407	28.733621571669033	26.910954156902868
4	11.733125037817738	16.16151336960169	35.5536241769485	36.55173741563206
5	13.499070376360056	37.65938369803184	32.650692542108104	16.190853383500006
6	33.278876090520036	35.85211833173812	16.88807111345809	13.98093446428375
7	29.00689245460496	31.408800118816455	21.124575753741894	18.45973167283669
8	29.073851493889947	31.49944579062162	19.896804288452916	19.529898427035516
9	28.246991679845973	13.577116138348346	18.72791033486901	39.44798184693667
10	16.493529711071687	26.483215754225288	30.714963488592762	26.30829104611026
11	37.57883474477208	21.179007472969964	21.625405347422966	19.616752434834986
12	24.956681500387802	24.48251582844224	28.241186404317	22.31961626685296
13	30.171320756792735	19.16900219204757	25.506687532832583	25.15298951832711
14	24.714787066679868	19.799333311330408	25.232405909986028	30.253473712003697
15	25.54347228181348	26.26351804790037	24.090178993806177	24.10283067647997
16	25.39797793106483	25.20462721541965	24.771994675291815	24.625400178223703
17	23.405062873362155	25.309141115768398	26.250316292066845	25.0354797188026
18	24.9881734270658	23.33602869181601	28.2938380804647	23.381959800653487
19	25.121841204880248	25.0236531458684	26.736305928688513	23.118199720562835
20	25.555573891327548	22.550249183141357	27.357063489444094	24.537113436086997
21	27.38566729375007	23.67954938006755	24.949943342464547	23.984839983717833
22	25.18867509378747	24.057449640802226	26.05311506430355	24.700760201106746
23	23.4749221646479	23.410013531799727	26.81276609789102	26.30229820566135
24	23.984289910558104	25.535771257577256	26.391960130697385	24.087978701167255
25	24.74174065150665	23.635818563868995	26.365831655610194	25.25660912901416
26	23.656446307358877	24.87100784404326	27.492931559897464	23.979614288700397
27	25.155670704203658	24.491182327249525	26.427164812920118	23.9259821556267
28	23.84017074270878	23.969437935245388	27.378791379253443	24.81159994279239
29	23.340979350253583	24.584694764403668	27.828476187332917	24.245849698009835
30	23.929007558005214	24.719462688537575	27.725887543043225	23.625642210413986
31	23.963387130488357	24.732389407791235	26.15460356227378	25.14961989944663
32	23.58933738187179	25.05968293783073	26.033312430553263	25.317667249744215
33	23.2785460466242	24.1688394556476	26.797914122578305	25.7547003751499
34	24.022519995159357	24.899336611769364	27.34963750178774	23.72850589128354
35	25.541547025754426	24.480455900634784	26.822942451346027	23.15505462226476
36	23.910030033994524	25.83226069067186	26.033312430553263	24.224396844780355
37	24.976621890711463	25.605905585442862	26.053390100883416	23.364082422962255
38	24.06295037239953	25.102313607709824	26.234089133854805	24.600646886035843
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	250.0
1	195.5
2	141.0
3	141.0
4	517.5
5	894.0
6	894.0
7	1042.5
8	1191.0
9	1126.0
10	1061.0
11	1061.0
12	1339.5
13	1618.0
14	1862.5
15	2107.0
16	2107.0
17	3028.5
18	3950.0
19	3950.0
20	4195.5
21	4441.0
22	3949.0
23	3457.0
24	3457.0
25	3477.5
26	3498.0
27	3498.0
28	4622.5
29	5747.0
30	7064.0
31	8381.0
32	8381.0
33	10131.5
34	11882.0
35	11882.0
36	12768.5
37	13655.0
38	15941.0
39	18227.0
40	18227.0
41	19387.5
42	20548.0
43	23654.5
44	26761.0
45	26761.0
46	30347.5
47	33934.0
48	33934.0
49	34160.0
50	34386.0
51	32969.0
52	31552.0
53	31552.0
54	29816.0
55	28080.0
56	28080.0
57	27593.5
58	27107.0
59	24491.5
60	21876.0
61	21876.0
62	21384.0
63	20892.0
64	17245.5
65	13599.0
66	13599.0
67	11555.5
68	9512.0
69	9512.0
70	7693.5
71	5875.0
72	4649.0
73	3423.0
74	3423.0
75	2522.5
76	1622.0
77	1622.0
78	1619.0
79	1616.0
80	1282.0
81	948.0
82	948.0
83	941.5
84	935.0
85	935.0
86	580.0
87	225.0
88	192.0
89	159.0
90	159.0
91	92.5
92	26.0
93	16.0
94	6.0
95	6.0
96	4.5
97	3.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009626280295279272
2	0.0
3	0.0
4	5.500731597302441E-4
5	0.0
6	0.0
7	5.500731597302441E-4
8	0.0019252560590558544
9	0.003575475538246587
10	8.251097395953663E-4
11	0.003025402378516343
12	5.500731597302441E-4
13	2.7503657986512205E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	363588.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.37134200195268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.98262595880004	40.792035483869284
2	11.837448804248519	11.925363642988158
3	3.1076017973854544	4.696022188259567
4	1.188680055064778	2.395016383382715
5	0.6319696095647965	1.5916578669114438
6	0.36811947853436644	1.1125603290501032
7	0.26531154285987124	0.9354868923722213
8	0.17165838893312113	0.6917330733163564
9	0.13667339674370027	0.6195980168950944
>10	0.9885134913611318	10.263617591721994
>50	0.17082796025440136	6.11384459790835
>100	0.14071405700442674	13.876948970965197
>500	0.00657030616363082	1.9490499717348568
>1k	0.00328515308181541	3.037064990624627
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3101	0.8528884341617434	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2304	0.6336842800092413	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1697	0.4667370760311121	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1475	0.405678955301055	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1425	0.3919271263077989	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1010	0.27778694566377327	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	700	0.19252560590558543	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	663	0.18234925245057593	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	649	0.1784987403324642	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	638	0.17547333795394787	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	616	0.1694225331969152	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	607	0.1669472039781291	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	575	0.15814603342244518	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	550	0.15127011892581713	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	523	0.14384413126945886	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	518	0.14246894837013324	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	514	0.14136880205067273	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	514	0.14136880205067273	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	491	0.13504296071377495	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	470	0.12926719253660737	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	469	0.12899215595674224	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	451	0.12404149751917004	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	450	0.12376646093930493	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	450	0.12376646093930493	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	446	0.12266631461984444	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	433	0.11909083908159786	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	431	0.1185407659218676	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	427	0.1174406196024071	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	426	0.117165583022542	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	420	0.11551536354335126	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	420	0.11551536354335126	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	418	0.11496529038362102	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	410	0.11276499774470004	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	403	0.11083974168564419	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	393	0.10808937588699297	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	393	0.10808937588699297	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	389	0.10698922956753247	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	365	0.10038835165076955	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	2.7503657986512205E-4	0.0
14	0.0	0.0	0.0	2.7503657986512205E-4	0.0
15	0.0	0.0	0.0	5.500731597302441E-4	0.0
16	0.0	0.0	0.0	0.0011001463194604882	0.0
17	0.0	0.0	0.0	0.0011001463194604882	0.0
18	0.0	0.0	0.0	0.0013751828993256103	0.0
19	0.0	0.0	0.0	0.0013751828993256103	0.0
20	0.0	0.0	0.0	0.0016502194791907323	2.7503657986512205E-4
21	0.0	0.0	0.0	0.0027503657986512205	2.7503657986512205E-4
22	0.0	0.0	0.0	0.0033004389583814646	2.7503657986512205E-4
23	0.0	0.0	0.0	0.006600877916762929	2.7503657986512205E-4
24	0.0	0.0	0.0	0.00935124371541415	2.7503657986512205E-4
25	0.0	0.0	0.0	0.011276499774470004	2.7503657986512205E-4
26	0.0	0.0	0.0	0.015402048472446835	5.500731597302441E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTATTC	75	2.4792826E-9	23.46586	3
GTAATAT	35	0.0020642534	22.856356	3
CGTCTTA	45	3.5311692E-4	21.332598	15
TGTAATA	45	3.5311692E-4	21.332598	2
CGTTATT	85	1.0510121E-8	20.705172	2
TTCTCGT	55	5.8146303E-5	20.365738	11
ACCTTGT	40	0.004475015	20.002062	1
TAGGGTC	50	7.1846606E-4	19.19934	4
GTCTTAT	50	7.1846606E-4	19.19934	16
CCATAGG	60	1.1345675E-4	18.668592	1
ATACCGT	120	8.185452E-11	18.668592	6
TAATACT	60	1.13576534E-4	18.666025	4
CAGATAC	120	8.185452E-11	18.666025	3
TATTCCC	105	5.9299055E-9	18.2876	5
CAAGACG	185	0.0	18.16154	4
TACCGTC	115	9.331416E-10	18.088823	7
GTGTATA	80	2.7827846E-6	18.001856	1
CCCTAGA	45	0.008833715	17.779612	1
AACCATC	45	0.008833715	17.779612	7
TAGGCTG	45	0.008833715	17.779612	5
>>END_MODULE