FastQCFastQC Report
Thu 2 Feb 2017
SRR4062889_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062889_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954273
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT21700.22739823928791866No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16350.1713346180809894No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT16340.17122982626564934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT14570.15268167495045967No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT12490.1308849773597283No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12040.12616934566942584No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11580.12134892216378332No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11480.12030100401038278No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11450.11998662856436261No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11170.11705245773484109No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC10800.11317516056725904No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10640.11149849152181816No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10500.11003140610705742No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10500.11003140610705742No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAG555.8312235E-520.3639713
GCATCGT2050.020.29195227
CGCTTCG1900.020.209832
TACACCG400.004486586419.9971855
ATCGTTT2050.019.5104729
CATCGTT2100.019.04693228
ACGCTTA601.1377821E-418.66697317
TAGTACG701.784073E-518.288892
CAAGACG3250.018.212824
ACCCGTT1159.404175E-1018.08630630
CGTTTAT2400.017.99935231
AACGTAT450.00885104117.77713831
CGGCATC2250.017.77713825
AACGCTT2250.017.77713830
GGCATCG2250.017.77713826
AGATCGA450.00885638717.7752746
GTAGGAC7400.017.728163
TCTAGCG2800.017.71364828
CGCTTAC550.001364265317.45483218
ACGTATG550.001364744517.45391732
Produced by FastQC (version 0.11.5)