##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062889_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	954273
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25707108971961	32.0	32.0	32.0	32.0	32.0
2	30.832337287128524	32.0	32.0	32.0	32.0	32.0
3	30.889215140740646	32.0	32.0	32.0	32.0	32.0
4	30.957942852831422	32.0	32.0	32.0	32.0	32.0
5	30.848608312296378	32.0	32.0	32.0	32.0	32.0
6	34.53767003781937	36.0	36.0	36.0	32.0	36.0
7	34.46800758273576	36.0	36.0	36.0	32.0	36.0
8	34.42562243718517	36.0	36.0	36.0	32.0	36.0
9	34.59475328338955	36.0	36.0	36.0	32.0	36.0
10	34.27589065183653	36.0	36.0	36.0	32.0	36.0
11	34.59250864270497	36.0	36.0	36.0	32.0	36.0
12	34.37538104923853	36.0	36.0	36.0	32.0	36.0
13	34.484564689559484	36.0	36.0	36.0	32.0	36.0
14	34.372297026113074	36.0	36.0	36.0	32.0	36.0
15	34.30443908608962	36.0	36.0	36.0	32.0	36.0
16	34.32419234328122	36.0	36.0	36.0	32.0	36.0
17	34.23464983290945	36.0	36.0	36.0	32.0	36.0
18	34.272465007393066	36.0	36.0	36.0	32.0	36.0
19	34.252832260789106	36.0	36.0	36.0	32.0	36.0
20	34.238198083776865	36.0	36.0	36.0	32.0	36.0
21	34.209974504151326	36.0	36.0	36.0	32.0	36.0
22	34.18527821703014	36.0	36.0	36.0	32.0	36.0
23	34.12355478987669	36.0	36.0	36.0	32.0	36.0
24	34.11268787862593	36.0	36.0	36.0	32.0	36.0
25	34.07958938375077	36.0	36.0	36.0	32.0	36.0
26	34.031990845387014	36.0	36.0	36.0	32.0	36.0
27	34.035184899918576	36.0	36.0	36.0	32.0	36.0
28	34.009704770018644	36.0	36.0	36.0	32.0	36.0
29	33.96549939063559	36.0	36.0	36.0	32.0	36.0
30	33.93083321020295	36.0	36.0	36.0	32.0	36.0
31	33.94303412126299	36.0	36.0	36.0	32.0	36.0
32	33.90142967473668	36.0	36.0	36.0	32.0	36.0
33	33.8792001869486	36.0	36.0	36.0	32.0	36.0
34	33.88200546384525	36.0	36.0	36.0	32.0	36.0
35	33.828959846920114	36.0	36.0	36.0	32.0	36.0
36	33.77865558388427	36.0	36.0	36.0	32.0	36.0
37	33.76986564641355	36.0	36.0	36.0	32.0	36.0
38	33.14274636293807	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	7.0
11	12.0
12	3.0
13	6.0
14	80.0
15	229.0
16	326.0
17	347.0
18	446.0
19	624.0
20	859.0
21	1100.0
22	1796.0
23	2747.0
24	4210.0
25	6315.0
26	9556.0
27	13661.0
28	19134.0
29	25689.0
30	34767.0
31	46910.0
32	63878.0
33	95433.0
34	209806.0
35	416328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.01223131859658	16.975387330334783	11.121812727999522	25.89056862306912
2	16.602202848710114	19.214900945201386	38.371925998542615	25.810970207545886
3	19.710132168747556	23.35449332868002	27.963997614653323	28.9713768879191
4	12.22905252744581	14.882985902976598	36.40725013728145	36.48071143229614
5	14.20330283535809	36.9401354541844	34.042006935136875	14.814554775320637
6	34.60397035646021	35.546049763589416	16.67836843164213	13.17161144830824
7	30.6283422039605	30.152901737762672	20.572414812113514	18.64634124616331
8	28.193622552969867	33.73901908162164	19.18054552751897	18.886812837889526
9	27.385169008416888	14.13412528084236	18.594069615371716	39.886636095369035
10	15.971651688407498	26.3269801482177	31.826355588343784	25.875012575031022
11	38.075237514382906	20.88229807871666	22.178698004103712	18.863766402796728
12	25.219674907335698	23.63371901103588	28.740341901659	22.40626417996942
13	29.940574758421196	19.38761607311296	25.15280776405978	25.519001404406062
14	23.062928048544045	20.247960301199452	24.908377306287566	31.780734343968938
15	24.806721561656243	28.492428180918683	22.071580103640844	24.629270153784233
16	25.448243915117676	25.75688977203176	24.766288536190693	24.028577776659876
17	23.73243754552744	26.556999040976002	25.572820316635138	24.137743096861424
18	24.817418041162025	24.412169405876366	27.4633237933452	23.307088759616402
19	25.56809071425952	25.021143255380135	25.34906021242815	24.061705817932204
20	25.74702139856197	24.399223242365778	25.572114694363897	24.281640664708355
21	26.48721100711682	24.380985916230973	24.238670577718533	24.89313249893368
22	25.664530165330827	25.282328381233445	25.20016600154264	23.852975451893087
23	23.54202495747671	24.93903161133661	26.149176835051556	25.369766596135122
24	24.586925193239882	25.217136119481196	25.900589545394997	24.295349141883925
25	24.470583796326867	25.156663176528664	25.805954615628902	24.56679841151557
26	23.61821632912732	26.31727335708319	26.35395671992043	23.710553593869058
27	24.782512552492	25.420224210406072	25.463297257473066	24.33396597962886
28	23.60024857921052	25.411565221127557	26.07723771068188	24.91094848898004
29	23.84848529310045	25.439383427410817	25.811221805575062	24.90090947391367
30	23.668240336601187	25.59719988053383	26.543288149269838	24.191271633595147
31	24.958605107396167	25.23097302100974	24.924022032562583	24.886399839031505
32	24.151529473903736	25.663554516425506	25.08632732386868	25.098588685802074
33	23.508254550141952	25.283981048018184	26.265744848307644	24.942019553532223
34	24.53283810154627	25.59177333923028	26.222061989213618	23.65332657000983
35	25.175396857005705	25.268990757995308	26.091631887746015	23.463980497252972
36	23.513678372220255	25.806857317735243	26.108596905675725	24.57086740436878
37	24.996174782067378	25.750109516513415	25.111036120537335	24.142679580881875
38	23.911626434938622	25.778906795274054	25.60693423542949	24.702532534357832
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	83.0
2	102.0
3	102.0
4	250.5
5	399.0
6	399.0
7	568.0
8	737.0
9	751.0
10	765.0
11	765.0
12	1007.0
13	1249.0
14	1642.0
15	2035.0
16	2035.0
17	2947.5
18	3860.0
19	3860.0
20	4794.0
21	5728.0
22	6414.0
23	7100.0
24	7100.0
25	8741.5
26	10383.0
27	10383.0
28	13580.0
29	16777.0
30	21679.0
31	26581.0
32	26581.0
33	33584.0
34	40587.0
35	40587.0
36	44902.0
37	49217.0
38	54601.5
39	59986.0
40	59986.0
41	61302.0
42	62618.0
43	68254.5
44	73891.0
45	73891.0
46	75982.5
47	78074.0
48	78074.0
49	81033.5
50	83993.0
51	84527.5
52	85062.0
53	85062.0
54	80323.5
55	75585.0
56	75585.0
57	72805.5
58	70026.0
59	62380.5
60	54735.0
61	54735.0
62	52497.0
63	50259.0
64	42093.5
65	33928.0
66	33928.0
67	28500.5
68	23073.0
69	23073.0
70	18582.5
71	14092.0
72	11193.5
73	8295.0
74	8295.0
75	6363.5
76	4432.0
77	4432.0
78	4117.0
79	3802.0
80	3035.5
81	2269.0
82	2269.0
83	2224.0
84	2179.0
85	2179.0
86	1470.0
87	761.0
88	649.5
89	538.0
90	538.0
91	397.0
92	256.0
93	210.0
94	164.0
95	164.0
96	155.5
97	147.0
98	147.0
99	335.5
100	524.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12009142037970265
2	0.05323424219274778
3	0.011946266948766233
4	0.0030389626448615857
5	2.0958363068010938E-4
6	1.0479181534005469E-4
7	0.0
8	4.1916726136021875E-4
9	1.0479181534005469E-4
10	1.0479181534005469E-4
11	0.00157187723010082
12	0.002305419937481203
13	0.01393731144022727
14	0.009221679749924811
15	0.02190148940607143
16	0.011003140610705741
17	0.018652943130529735
18	0.004610839874962406
19	0.007125843443123719
20	0.005239590767002734
21	0.005658758028362953
22	0.006811467997103554
23	0.009431263380604921
24	0.015509188670328092
25	0.016347523193048528
26	0.01697627408508886
27	0.008383345227204374
28	0.005239590767002734
29	0.009955222457305195
30	0.002934170829521531
31	0.0051347989516626795
32	0.005868341659043062
33	0.007545010704483938
34	0.01121272424138585
35	0.015823564116348256
36	0.014566062332267602
37	0.00827855341186432
38	0.0053443825823427885
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	954273.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.20726175731827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.98443767267037	46.32897924209681
2	12.267165684816078	14.035419167033316
3	3.323571200230672	5.703972228620416
4	1.222890013280884	2.798327563606798
5	0.5968226728634429	1.7071295434600657
6	0.34481410877026325	1.183552258682212
7	0.21052967879494042	0.8430678509754408
8	0.1356097084215134	0.6206288069202501
9	0.10572991746690473	0.5443667157697989
>10	0.6262979419183432	7.021176193691563
>50	0.08243985381380022	3.3306691346584447
>100	0.08775793645431185	10.357235133931836
>500	0.00936329439097619	3.6180425930683198
>1k	0.0025703161073267976	1.9074335674847236
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2170	0.22739823928791866	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1635	0.1713346180809894	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1634	0.17122982626564934	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1457	0.15268167495045967	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1249	0.1308849773597283	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1204	0.12616934566942584	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1158	0.12134892216378332	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1148	0.12030100401038278	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1145	0.11998662856436261	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1117	0.11705245773484109	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1080	0.11317516056725904	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1064	0.11149849152181816	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1050	0.11003140610705742	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1050	0.11003140610705742	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.0958363068010935E-4
7	0.0	0.0	0.0	0.0	2.0958363068010935E-4
8	0.0	0.0	0.0	0.0	2.0958363068010935E-4
9	0.0	0.0	0.0	2.0958363068010935E-4	2.0958363068010935E-4
10	0.0	0.0	0.0	3.1437544602016404E-4	2.0958363068010935E-4
11	0.0	0.0	0.0	3.1437544602016404E-4	2.0958363068010935E-4
12	0.0	0.0	0.0	3.1437544602016404E-4	3.1437544602016404E-4
13	0.0	0.0	0.0	3.1437544602016404E-4	4.191672613602187E-4
14	0.0	0.0	0.0	3.1437544602016404E-4	4.191672613602187E-4
15	0.0	0.0	0.0	4.191672613602187E-4	4.191672613602187E-4
16	0.0	0.0	0.0	6.287508920403281E-4	5.239590767002734E-4
17	0.0	0.0	0.0	6.287508920403281E-4	5.239590767002734E-4
18	0.0	0.0	0.0	9.431263380604921E-4	5.239590767002734E-4
19	0.0	0.0	0.0	9.431263380604921E-4	5.239590767002734E-4
20	0.0	0.0	0.0	0.0012575017840806561	5.239590767002734E-4
21	0.0	0.0	0.0	0.0015718772301008203	5.239590767002734E-4
22	0.0	0.0	0.0	0.0020958363068010934	6.287508920403281E-4
23	0.0	0.0	0.0	0.002410211752821258	6.287508920403281E-4
24	0.0	0.0	0.0	0.0033533380908817496	6.287508920403281E-4
25	0.0	0.0	0.0	0.003562921721561859	6.287508920403281E-4
26	0.0	0.0	0.0	0.004820423505642516	6.287508920403281E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGTAG	55	5.8312235E-5	20.36397	13
GCATCGT	205	0.0	20.291952	27
CGCTTCG	190	0.0	20.2098	32
TACACCG	40	0.0044865864	19.997185	5
ATCGTTT	205	0.0	19.51047	29
CATCGTT	210	0.0	19.046932	28
ACGCTTA	60	1.1377821E-4	18.666973	17
TAGTACG	70	1.784073E-5	18.28889	2
CAAGACG	325	0.0	18.21282	4
ACCCGTT	115	9.404175E-10	18.086306	30
CGTTTAT	240	0.0	17.999352	31
AACGTAT	45	0.008851041	17.777138	31
CGGCATC	225	0.0	17.777138	25
AACGCTT	225	0.0	17.777138	30
GGCATCG	225	0.0	17.777138	26
AGATCGA	45	0.008856387	17.775274	6
GTAGGAC	740	0.0	17.72816	3
TCTAGCG	280	0.0	17.713648	28
CGCTTAC	55	0.0013642653	17.454832	18
ACGTATG	55	0.0013647445	17.453917	32
>>END_MODULE