FastQCFastQC Report
Thu 2 Feb 2017
SRR4062889_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062889_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954273
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT24040.25191952407749146No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17150.17971796330819376No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17010.17825087789343302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT16550.1734304543877905No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13030.13654373538809125No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT12710.1331903972972095No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12660.13266643822050922No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12420.1301514346523479No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12010.1258549702234057No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11750.12313038302456424No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11030.11558537232008032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10960.11485182961269993No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC10760.11275599330589883No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10720.11233682604453862No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10610.11118411607579802No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC10280.10772598616957621No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10050.10531577441675495No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9710.10175285269519309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT601.7620187E-724.0009826
AGCGGCG1950.022.15359331
CTAGCGG2000.020.7997629
GAGCGTT707.778435E-720.5722717
TAGCGGC2050.020.2924530
TCTAGCG2150.020.0927928
CTATACC507.1927346E-419.2007854
GTCTTAC507.1952713E-419.1997781
GTAGGAC7150.018.7979743
GTAATAC701.786113E-518.2864633
GTAGTAC450.008847116517.7785073
ATCGTTT2250.017.77757529
CATCGTT2250.017.77757528
CGAGAAT450.008849787517.77757531
TGTAGGA7900.017.6200522
TAGGACG6800.017.4124774
AAGACGG4250.017.3183565
GTTTTCG3800.017.2629628
CAAGACG4500.017.0673664
TTCGGAA3700.016.86467231
Produced by FastQC (version 0.11.5)