##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062889_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	954273
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.205862473317385	32.0	32.0	32.0	32.0	32.0
2	31.30967134142955	32.0	32.0	32.0	32.0	32.0
3	31.40148888211235	32.0	32.0	32.0	32.0	32.0
4	31.49657173576115	32.0	32.0	32.0	32.0	32.0
5	31.418067995217303	32.0	32.0	32.0	32.0	32.0
6	34.97215576674599	36.0	36.0	36.0	36.0	36.0
7	35.00241649926174	36.0	36.0	36.0	36.0	36.0
8	34.9319272367551	36.0	36.0	36.0	36.0	36.0
9	35.04610735083147	36.0	36.0	36.0	36.0	36.0
10	34.897842650897594	36.0	36.0	36.0	32.0	36.0
11	35.068465732552426	36.0	36.0	36.0	36.0	36.0
12	34.97606135770372	36.0	36.0	36.0	36.0	36.0
13	35.02016823278035	36.0	36.0	36.0	36.0	36.0
14	34.9668103362455	36.0	36.0	36.0	36.0	36.0
15	34.93551950018496	36.0	36.0	36.0	36.0	36.0
16	34.939787670823755	36.0	36.0	36.0	36.0	36.0
17	34.91478748743808	36.0	36.0	36.0	32.0	36.0
18	34.91557133021683	36.0	36.0	36.0	32.0	36.0
19	34.907208943352686	36.0	36.0	36.0	32.0	36.0
20	34.89169032341898	36.0	36.0	36.0	32.0	36.0
21	34.8738233189035	36.0	36.0	36.0	32.0	36.0
22	34.85713312647429	36.0	36.0	36.0	32.0	36.0
23	34.80212266301153	36.0	36.0	36.0	32.0	36.0
24	34.77977790422657	36.0	36.0	36.0	32.0	36.0
25	34.75485107511163	36.0	36.0	36.0	32.0	36.0
26	34.69339591500545	36.0	36.0	36.0	32.0	36.0
27	34.67542831034725	36.0	36.0	36.0	32.0	36.0
28	34.63602134818862	36.0	36.0	36.0	32.0	36.0
29	34.598650491002054	36.0	36.0	36.0	32.0	36.0
30	34.57230687654371	36.0	36.0	36.0	32.0	36.0
31	34.56916836167428	36.0	36.0	36.0	32.0	36.0
32	34.545595442813536	36.0	36.0	36.0	32.0	36.0
33	34.52005662949701	36.0	36.0	36.0	32.0	36.0
34	34.49943569607439	36.0	36.0	36.0	32.0	36.0
35	34.46612971340486	36.0	36.0	36.0	32.0	36.0
36	34.427548510751116	36.0	36.0	36.0	32.0	36.0
37	34.398644832244024	36.0	36.0	36.0	32.0	36.0
38	33.97971754414093	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	12.0
22	61.0
23	220.0
24	576.0
25	1388.0
26	2989.0
27	5767.0
28	10305.0
29	16613.0
30	25285.0
31	37108.0
32	54015.0
33	87232.0
34	217290.0
35	495409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.39373297002725	18.27394676168518	11.749214001257597	26.583106267029972
2	15.599100047889861	20.013560060905004	37.70566703658177	26.68167285462336
3	18.688886723191374	24.07057519179522	28.259942385459922	28.98059569955348
4	11.819179247823731	15.684538249616722	36.387986221943244	36.1082962806163
5	13.681200243536178	37.42639684870053	33.70628740412859	15.186115503634703
6	34.723783914632214	35.92291917075967	16.30847002581028	13.044826888797836
7	30.43474160298784	30.244962631042853	20.54936349117858	18.77093227479073
8	27.910062058688528	34.050749378050796	18.902120810095383	19.137067753165287
9	27.26099964893344	13.975802606276233	18.372308708022658	40.390889036767675
10	15.739046601066784	26.661217475138066	31.740283148375198	25.859452775419957
11	38.08422943387684	20.8753263112446	22.127439278075343	18.91300497680321
12	24.977417292799732	23.74432812516374	28.741341549037486	22.536913032999045
13	29.91173366894729	19.2987107435938	25.233817228020133	25.555738359438774
14	22.93829962704593	20.3492082454392	24.79300996674956	31.919482160765316
15	24.737575096434668	28.595380986363438	21.96247824259934	24.704565674602552
16	25.47006988566165	25.80886182465605	24.62806764940431	24.093000640277992
17	23.595868268304773	26.716044570054905	25.469860302030973	24.218226859609356
18	24.762201173039582	24.446253849789315	27.413014933881602	23.3785300432895
19	25.531373097635583	25.051950542454833	25.345577209037668	24.07109915087192
20	25.828562685939975	24.284140911458252	25.57025086112674	24.31704554147503
21	26.421949320476052	24.40501702346608	24.31018023182094	24.86285342423693
22	25.613110713600822	25.31927446338731	25.18073968350776	23.886875139504106
23	23.373259140494827	24.958764291028746	26.228740293627588	25.439236274848838
24	24.673442505446555	25.114720839843525	25.909566759197837	24.30226989551208
25	24.421418189553723	25.121532307840628	25.828038726863277	24.629010775742373
26	23.59754493735021	26.1839117317581	26.326952559697276	23.89159077119441
27	24.788713502320615	25.273061272822346	25.498782843064827	24.43944238179221
28	23.616197880480744	25.290875881430157	26.137279373931776	24.955646864157323
29	23.789628334868535	25.346101168114366	25.934402419433432	24.92986807758367
30	23.733669505476946	25.305546735577767	26.62592360886245	24.33486015008284
31	24.980482524392915	25.0680884820172	25.080558708042666	24.870870285547216
32	24.084198127789428	25.52414246237712	25.306175486469805	25.08548392336365
33	23.41415926050512	25.19562012128605	26.38092034459741	25.00930027361143
34	24.489952036786118	25.340023242824643	26.39202827702345	23.777996443365787
35	25.250531032524236	25.164077784868688	26.074509076543084	23.51088210606399
36	23.575224280682782	25.75154070166504	26.08750326164525	24.58573175600693
37	25.11042437541458	25.559247720516037	25.095963104897656	24.234364799171725
38	24.01024026692599	25.492626840146205	25.759112705811344	24.73802018711646
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	51.0
2	70.0
3	70.0
4	227.0
5	384.0
6	384.0
7	496.5
8	609.0
9	671.5
10	734.0
11	734.0
12	952.0
13	1170.0
14	1585.5
15	2001.0
16	2001.0
17	2993.5
18	3986.0
19	3986.0
20	4891.5
21	5797.0
22	6507.5
23	7218.0
24	7218.0
25	8744.5
26	10271.0
27	10271.0
28	13487.5
29	16704.0
30	21634.0
31	26564.0
32	26564.0
33	33668.0
34	40772.0
35	40772.0
36	44858.0
37	48944.0
38	54515.5
39	60087.0
40	60087.0
41	61483.0
42	62879.0
43	68378.0
44	73877.0
45	73877.0
46	76174.0
47	78471.0
48	78471.0
49	81696.0
50	84921.0
51	85160.0
52	85399.0
53	85399.0
54	80225.5
55	75052.0
56	75052.0
57	72622.0
58	70192.0
59	62622.5
60	55053.0
61	55053.0
62	52796.0
63	50539.0
64	42315.5
65	34092.0
66	34092.0
67	28542.5
68	22993.0
69	22993.0
70	18594.5
71	14196.0
72	11245.0
73	8294.0
74	8294.0
75	6248.0
76	4202.0
77	4202.0
78	4026.5
79	3851.0
80	2969.0
81	2087.0
82	2087.0
83	1974.5
84	1862.0
85	1862.0
86	1176.5
87	491.0
88	416.5
89	342.0
90	342.0
91	212.5
92	83.0
93	58.5
94	34.0
95	34.0
96	22.5
97	11.0
98	11.0
99	10.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007649802519823993
2	0.0
3	0.0
4	2.0958363068010938E-4
5	0.0
6	2.0958363068010938E-4
7	5.239590767002734E-4
8	0.00157187723010082
9	0.003982088982922078
10	3.1437544602016404E-4
11	0.004820423505642515
12	3.1437544602016404E-4
13	2.0958363068010938E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.0958363068010938E-4
22	0.0
23	3.1437544602016404E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.0479181534005469E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	954273.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.18587071570419
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.5221259370431	43.88497760999607
2	13.255698085426568	14.630544815775162
3	3.547039376127471	5.87239369303448
4	1.3328341816215252	2.9421445932974755
5	0.6484416292699637	1.7892407959786398
6	0.3349080940734334	1.1089316868707635
7	0.23197043783861	0.8961043414698779
8	0.1524972579737822	0.6732555170432426
9	0.10301660405579526	0.5116554893694604
>10	0.672762609546769	7.204908207138359
>50	0.09001202849832468	3.4709172190900777
>100	0.09515471149008951	10.850190805718292
>500	0.010297303378425741	3.8102212031114635
>1k	0.0032417436561710668	2.3545140221066614
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2404	0.25191952407749146	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1715	0.17971796330819376	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1701	0.17825087789343302	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1655	0.1734304543877905	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1303	0.13654373538809125	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1271	0.1331903972972095	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1266	0.13266643822050922	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1242	0.1301514346523479	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1201	0.1258549702234057	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1175	0.12313038302456424	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1103	0.11558537232008032	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1096	0.11485182961269993	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1076	0.11275599330589883	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1072	0.11233682604453862	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1061	0.11118411607579802	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1028	0.10772598616957621	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1005	0.10531577441675495	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	971	0.10175285269519309	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.0479181534005467E-4	0.0
11	0.0	0.0	0.0	1.0479181534005467E-4	0.0
12	0.0	0.0	0.0	1.0479181534005467E-4	2.0958363068010935E-4
13	0.0	0.0	0.0	1.0479181534005467E-4	3.1437544602016404E-4
14	0.0	0.0	0.0	2.0958363068010935E-4	3.1437544602016404E-4
15	0.0	0.0	0.0	3.1437544602016404E-4	4.191672613602187E-4
16	0.0	0.0	0.0	5.239590767002734E-4	5.239590767002734E-4
17	0.0	0.0	0.0	5.239590767002734E-4	5.239590767002734E-4
18	0.0	0.0	0.0	8.383345227204374E-4	6.287508920403281E-4
19	0.0	0.0	0.0	8.383345227204374E-4	6.287508920403281E-4
20	0.0	0.0	0.0	0.0011527099687406014	6.287508920403281E-4
21	0.0	0.0	0.0	0.0015718772301008203	6.287508920403281E-4
22	0.0	0.0	0.0	0.001991044491461039	6.287508920403281E-4
23	0.0	0.0	0.0	0.002410211752821258	6.287508920403281E-4
24	0.0	0.0	0.0	0.003248546275541695	6.287508920403281E-4
25	0.0	0.0	0.0	0.0034581299062218045	6.287508920403281E-4
26	0.0	0.0	0.0	0.004820423505642516	6.287508920403281E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACCGT	60	1.7620187E-7	24.000982	6
AGCGGCG	195	0.0	22.153593	31
CTAGCGG	200	0.0	20.79976	29
GAGCGTT	70	7.778435E-7	20.572271	7
TAGCGGC	205	0.0	20.29245	30
TCTAGCG	215	0.0	20.09279	28
CTATACC	50	7.1927346E-4	19.200785	4
GTCTTAC	50	7.1952713E-4	19.199778	1
GTAGGAC	715	0.0	18.797974	3
GTAATAC	70	1.786113E-5	18.286463	3
GTAGTAC	45	0.0088471165	17.778507	3
ATCGTTT	225	0.0	17.777575	29
CATCGTT	225	0.0	17.777575	28
CGAGAAT	45	0.0088497875	17.777575	31
TGTAGGA	790	0.0	17.620052	2
TAGGACG	680	0.0	17.412477	4
AAGACGG	425	0.0	17.318356	5
GTTTTCG	380	0.0	17.26296	28
CAAGACG	450	0.0	17.067366	4
TTCGGAA	370	0.0	16.864672	31
>>END_MODULE