FastQCFastQC Report
Thu 2 Feb 2017
SRR4062888_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062888_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137899
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32630.15262648048387692No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21380.10000472426433615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21800.020.5698851
TCTAGCG3250.020.18384728
CTAGCGG3250.019.69155929
GGTATCA7750.019.4247971
CAAGACG4950.017.7758544
TAGCGGC3650.017.53275930
AAGACGG5200.016.612815
CGCAAGA5200.015.6957492
TAGGACG6450.015.6262164
GTCCTAA5900.015.472241
CGCGTAA2200.015.272861510
CGTGATT4400.015.27214628
CGTCTTA4350.015.0805915
TGTAGGA8950.015.0200542
GTATAGG2350.014.9928831
AGTACGG650.004167426414.7669425
ATCGTTT2850.014.59593729
GCGCAAG6050.014.5592041
AGCGGCG4450.014.38080331
GTCCTAC7200.014.2356671
Produced by FastQC (version 0.11.5)