##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062888_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2137899
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.278309218536517	32.0	32.0	32.0	32.0	32.0
2	30.842491623785783	32.0	32.0	32.0	32.0	32.0
3	30.901296553298355	32.0	32.0	32.0	32.0	32.0
4	30.98376770839034	32.0	32.0	32.0	32.0	32.0
5	30.85512084527847	32.0	32.0	32.0	32.0	32.0
6	34.53386946717315	36.0	36.0	36.0	32.0	36.0
7	34.482132224207035	36.0	36.0	36.0	32.0	36.0
8	34.449927709400676	36.0	36.0	36.0	32.0	36.0
9	34.63580505907903	36.0	36.0	36.0	32.0	36.0
10	34.299454277306836	36.0	36.0	36.0	32.0	36.0
11	34.60955264958728	36.0	36.0	36.0	32.0	36.0
12	34.41189223625625	36.0	36.0	36.0	32.0	36.0
13	34.50990949525679	36.0	36.0	36.0	32.0	36.0
14	34.40700613078541	36.0	36.0	36.0	32.0	36.0
15	34.33258727376737	36.0	36.0	36.0	32.0	36.0
16	34.355969575737674	36.0	36.0	36.0	32.0	36.0
17	34.29568281756996	36.0	36.0	36.0	32.0	36.0
18	34.315791812428934	36.0	36.0	36.0	32.0	36.0
19	34.29541666842073	36.0	36.0	36.0	32.0	36.0
20	34.29627077799279	36.0	36.0	36.0	32.0	36.0
21	34.28321450171406	36.0	36.0	36.0	32.0	36.0
22	34.25518183974079	36.0	36.0	36.0	32.0	36.0
23	34.19564768962425	36.0	36.0	36.0	32.0	36.0
24	34.18374675323764	36.0	36.0	36.0	32.0	36.0
25	34.1646345313787	36.0	36.0	36.0	32.0	36.0
26	34.12241504392864	36.0	36.0	36.0	32.0	36.0
27	34.12098466765736	36.0	36.0	36.0	32.0	36.0
28	34.1012517429495	36.0	36.0	36.0	32.0	36.0
29	34.06779927396009	36.0	36.0	36.0	32.0	36.0
30	34.04499885167634	36.0	36.0	36.0	32.0	36.0
31	34.053658755628774	36.0	36.0	36.0	32.0	36.0
32	34.011813935083	36.0	36.0	36.0	32.0	36.0
33	33.99077318432723	36.0	36.0	36.0	32.0	36.0
34	33.9996707047433	36.0	36.0	36.0	32.0	36.0
35	33.97199587071232	36.0	36.0	36.0	32.0	36.0
36	33.92165298734879	36.0	36.0	36.0	32.0	36.0
37	33.92413392774869	36.0	36.0	36.0	32.0	36.0
38	33.3321279443042	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	7.0
8	6.0
9	6.0
10	25.0
11	14.0
12	13.0
13	9.0
14	143.0
15	392.0
16	554.0
17	663.0
18	775.0
19	1093.0
20	1498.0
21	2229.0
22	3410.0
23	5472.0
24	8643.0
25	13432.0
26	19938.0
27	29294.0
28	41262.0
29	55814.0
30	75923.0
31	102113.0
32	140397.0
33	210663.0
34	469384.0
35	954726.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.95111016615378	17.588210803953146	11.43674153320055	26.02393749669252
2	16.596122669801677	19.081230741582456	38.19671947014314	26.12592711847273
3	19.131524965441102	22.796803038843965	28.76352825876954	29.30814373694539
4	12.361729596227969	15.336133667627138	36.657139916925495	35.6449968192194
5	14.268527812885019	36.60270340812033	34.574211817798265	14.554556961196392
6	34.322515522285016	36.19842705017181	16.527245971970547	12.951811455572635
7	30.290486126800193	30.535726898230457	20.690687445945763	18.48309952902359
8	27.662453628278765	34.140251566149196	19.040763146040927	19.15653165953112
9	26.86114493208494	14.370798137042534	18.853861118270295	39.91419581260223
10	15.56846342601991	26.74459723296752	32.53007877875621	25.156860562256362
11	37.12279293552264	21.388394233274443	22.5106426968016	18.97817013440132
12	24.215092673325124	23.972931455792597	29.341173675012815	22.47080219586946
13	29.371265664793533	19.68711580672868	25.33214882512879	25.609469703348996
14	22.806114787695016	20.109903012719727	25.482959980876668	31.601022218708586
15	24.940359308556303	28.008550643473818	22.197753046599512	24.853337001370367
16	25.422996983154032	26.24342564754126	24.115400790489428	24.218176578815275
17	23.721657896115754	26.435819029725057	25.520525334790516	24.32199773936867
18	24.30423895588247	25.47822096608283	26.64345553093485	23.574084547099847
19	25.187769796288013	25.378814103223803	25.55147437180852	23.881941728679664
20	25.246916179008966	25.218381771659253	25.37620978870505	24.158492260626726
21	26.06462302456038	24.68747544149181	24.921599886235633	24.326301647712175
22	25.289254883909784	25.086937851014206	25.4099434260764	24.213863838999615
23	24.302374536881103	25.024465775981437	25.866032334119232	24.807127353018224
24	24.608146437331264	25.382239963847255	25.7480713155941	24.26154228322738
25	24.566499543166657	25.187399914200476	25.80203139963163	24.444069143001236
26	24.09261521345841	25.88616830308834	26.1978892055117	23.823327277941548
27	24.68293025214015	25.586798896009732	25.329092014782244	24.401178837067878
28	24.050319678618674	25.366970846282953	26.028874922115175	24.5538345529832
29	24.263880695881436	25.463882754204008	25.75167367353975	24.520562876374807
30	24.20342122619666	25.462829130473423	26.035325540384406	24.29842410294551
31	24.86651978463539	25.098163039054715	25.384723333192998	24.650593843116894
32	24.29355829196597	25.548846547501213	25.255641897391467	24.901953263141348
33	24.148904718868174	25.28820479427576	25.781956069000746	24.78093441785532
34	24.724320086226577	25.285176438887287	26.096544574724085	23.893958900162048
35	24.93650414190886	25.329432277556315	25.876322372296016	23.857741208238814
36	24.048684665415728	25.50869131775787	25.733897893565334	24.708726123261073
37	25.00060813592306	25.24302047079076	25.21149096216459	24.54488043112159
38	24.23638706780212	25.484980809396728	25.710309599043864	24.56832252375729
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	60.5
2	96.0
3	96.0
4	289.0
5	482.0
6	482.0
7	614.0
8	746.0
9	835.0
10	924.0
11	924.0
12	1341.5
13	1759.0
14	2851.0
15	3943.0
16	3943.0
17	5876.5
18	7810.0
19	7810.0
20	10197.5
21	12585.0
22	15724.0
23	18863.0
24	18863.0
25	23278.5
26	27694.0
27	27694.0
28	35352.5
29	43011.0
30	51706.5
31	60402.0
32	60402.0
33	74390.0
34	88378.0
35	88378.0
36	96936.0
37	105494.0
38	117436.0
39	129378.0
40	129378.0
41	135510.0
42	141642.0
43	154366.0
44	167090.0
45	167090.0
46	170367.5
47	173645.0
48	173645.0
49	182494.0
50	191343.0
51	192124.5
52	192906.0
53	192906.0
54	185964.0
55	179022.0
56	179022.0
57	169326.5
58	159631.0
59	144001.5
60	128372.0
61	128372.0
62	118300.0
63	108228.0
64	90600.5
65	72973.0
66	72973.0
67	60762.0
68	48551.0
69	48551.0
70	38870.5
71	29190.0
72	22810.0
73	16430.0
74	16430.0
75	12671.5
76	8913.0
77	8913.0
78	7918.5
79	6924.0
80	5522.0
81	4120.0
82	4120.0
83	3734.5
84	3349.0
85	3349.0
86	2325.5
87	1302.0
88	1050.0
89	798.0
90	798.0
91	614.0
92	430.0
93	357.0
94	284.0
95	284.0
96	259.5
97	235.0
98	235.0
99	583.0
100	931.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12105342675215246
2	0.054773401362739775
3	0.011413074237838177
4	0.002759718770624805
5	2.3387447208684788E-4
6	2.3387447208684788E-4
7	0.0
8	5.612987330084349E-4
9	3.741991553389566E-4
10	6.080736274258044E-4
11	0.0022451949320337395
12	0.0031339179259637618
13	0.01417279300846298
14	0.009167879305804436
15	0.0241358455193627
16	0.01103887508249922
17	0.020721278226894724
18	0.006174286063092785
19	0.008185606523039676
20	0.006033961379840675
21	0.006875909479353327
22	0.00771785757886598
23	0.010243701877403937
24	0.01412601811404561
25	0.016745412201418307
26	0.019177706711121525
27	0.009308203989056546
28	0.0059404115910059365
29	0.01103887508249922
30	0.0032274677147985007
31	0.006033961379840675
32	0.0066888099016838495
33	0.008559805678378631
34	0.012863095964776633
35	0.016745412201418307
36	0.014313117691715092
37	0.010243701877403937
38	0.0062678358519275236
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2137899.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.03235033345709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.63256256438504	38.3127529130028
2	14.737336406406031	15.336365017602605
3	5.303439012613413	8.278511900292745
4	2.360983016693192	4.913899818236901
5	1.2049686731131553	3.1348676070132315
6	0.7032937912079669	2.1956417358886893
7	0.42240117417059647	1.5384968112995672
8	0.30935201821960834	1.2877050070691727
9	0.21353707952374837	0.9999752517866664
>10	0.9527224398466814	8.742318299328094
>50	0.07787616887058657	2.8253357976255007
>100	0.07095543408726332	7.7891911834120044
>500	0.007771034138563217	2.664352573967009
>1k	0.002801186724365787	1.9805860834749685
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3263	0.15262648048387692	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2138	0.10000472426433615	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.354978883473916E-5
2	0.0	0.0	0.0	0.0	9.354978883473916E-5
3	0.0	0.0	0.0	0.0	9.354978883473916E-5
4	0.0	0.0	0.0	0.0	9.354978883473916E-5
5	0.0	0.0	0.0	0.0	9.354978883473916E-5
6	0.0	0.0	0.0	0.0	9.354978883473916E-5
7	0.0	0.0	0.0	0.0	9.354978883473916E-5
8	0.0	0.0	0.0	0.0	9.354978883473916E-5
9	0.0	0.0	0.0	9.354978883473916E-5	9.354978883473916E-5
10	0.0	0.0	0.0	1.4032468325210872E-4	9.354978883473916E-5
11	0.0	0.0	0.0	1.4032468325210872E-4	9.354978883473916E-5
12	0.0	0.0	0.0	2.3387447208684788E-4	3.2742426092158706E-4
13	0.0	0.0	0.0	2.3387447208684788E-4	5.145238385910653E-4
14	0.0	0.0	0.0	2.3387447208684788E-4	5.145238385910653E-4
15	0.0	0.0	0.0	2.3387447208684788E-4	6.548485218431741E-4
16	0.0	0.0	0.0	2.3387447208684788E-4	6.548485218431741E-4
17	0.0	0.0	0.0	2.8064936650421744E-4	6.548485218431741E-4
18	0.0	0.0	0.0	3.2742426092158706E-4	7.016234162605437E-4
19	0.0	0.0	0.0	4.209740497563262E-4	7.016234162605437E-4
20	0.0	0.0	0.0	4.6774894417369575E-4	8.419480995126524E-4
21	0.0	0.0	0.0	6.080736274258044E-4	8.419480995126524E-4
22	0.0	0.0	0.0	7.951732050952828E-4	8.419480995126524E-4
23	0.0	0.0	0.0	8.419480995126524E-4	8.419480995126524E-4
24	0.0	0.0	0.0	0.0010758225715995003	8.419480995126524E-4
25	0.0	0.0	0.0	0.0013096970436863482	8.419480995126524E-4
26	0.0	0.0	0.0	0.0014967966213558265	8.887229939300219E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2180	0.0	20.569885	1
TCTAGCG	325	0.0	20.183847	28
CTAGCGG	325	0.0	19.691559	29
GGTATCA	775	0.0	19.424797	1
CAAGACG	495	0.0	17.775854	4
TAGCGGC	365	0.0	17.532759	30
AAGACGG	520	0.0	16.61281	5
CGCAAGA	520	0.0	15.695749	2
TAGGACG	645	0.0	15.626216	4
GTCCTAA	590	0.0	15.47224	1
CGCGTAA	220	0.0	15.2728615	10
CGTGATT	440	0.0	15.272146	28
CGTCTTA	435	0.0	15.08059	15
TGTAGGA	895	0.0	15.020054	2
GTATAGG	235	0.0	14.992883	1
AGTACGG	65	0.0041674264	14.766942	5
ATCGTTT	285	0.0	14.595937	29
GCGCAAG	605	0.0	14.559204	1
AGCGGCG	445	0.0	14.380803	31
GTCCTAC	720	0.0	14.235667	1
>>END_MODULE