FastQCFastQC Report
Thu 2 Feb 2017
SRR4062888_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062888_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137899
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32710.15300067963921588No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22300.10430801455073414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23450.019.310031
GGTATCA9800.019.2661651
TCTAGCG3000.019.19950928
TAGCGGC3250.017.72262430
CTAGCGG3250.017.72262429
TCCGTAT550.001364758217.4553248
TATACTG2600.017.2319415
CCGTCGT3100.016.5168679
CCGTATG908.584082E-616.0007139
TATGCGA703.698977E-415.9995912
GTCCTAC6750.015.8821891
ATACCGT3550.015.7753526
GTATAGG2850.015.7199991
TAAACGC3400.015.52901528
GTACCGT1255.140646E-815.3606856
CGTCGTA3450.015.30431410
TACGCTG1052.1854194E-615.2391325
TACCGTC3700.015.1358097
AACGAAT2150.014.88333931
TACCGTA650.004161902214.769897
Produced by FastQC (version 0.11.5)