##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062888_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2137899
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24512757618578	32.0	32.0	32.0	32.0	32.0
2	31.29613232430531	32.0	32.0	32.0	32.0	32.0
3	31.390592820334355	32.0	32.0	32.0	32.0	32.0
4	31.483640714551996	32.0	32.0	32.0	32.0	32.0
5	31.40564778785153	32.0	32.0	32.0	32.0	32.0
6	34.95038306299783	36.0	36.0	36.0	36.0	36.0
7	34.98442162141429	36.0	36.0	36.0	36.0	36.0
8	34.91113799108377	36.0	36.0	36.0	36.0	36.0
9	35.032506212875354	36.0	36.0	36.0	36.0	36.0
10	34.8782281108696	36.0	36.0	36.0	32.0	36.0
11	35.05333694435518	36.0	36.0	36.0	36.0	36.0
12	34.9590719673848	36.0	36.0	36.0	36.0	36.0
13	35.011414009735724	36.0	36.0	36.0	36.0	36.0
14	34.95918937236979	36.0	36.0	36.0	32.0	36.0
15	34.925934293434814	36.0	36.0	36.0	32.0	36.0
16	34.947212192905276	36.0	36.0	36.0	36.0	36.0
17	34.91811961182451	36.0	36.0	36.0	32.0	36.0
18	34.918477907515744	36.0	36.0	36.0	32.0	36.0
19	34.91788807609714	36.0	36.0	36.0	32.0	36.0
20	34.90223158343776	36.0	36.0	36.0	32.0	36.0
21	34.891804991723184	36.0	36.0	36.0	32.0	36.0
22	34.87803493055565	36.0	36.0	36.0	32.0	36.0
23	34.82724394370361	36.0	36.0	36.0	32.0	36.0
24	34.804960384003174	36.0	36.0	36.0	32.0	36.0
25	34.7954285960188	36.0	36.0	36.0	32.0	36.0
26	34.73331761696881	36.0	36.0	36.0	32.0	36.0
27	34.71524800750643	36.0	36.0	36.0	32.0	36.0
28	34.69062897732774	36.0	36.0	36.0	32.0	36.0
29	34.65941421928726	36.0	36.0	36.0	32.0	36.0
30	34.63662128098662	36.0	36.0	36.0	32.0	36.0
31	34.63955079262397	36.0	36.0	36.0	32.0	36.0
32	34.61080715225555	36.0	36.0	36.0	32.0	36.0
33	34.588497398614244	36.0	36.0	36.0	32.0	36.0
34	34.58865877199999	36.0	36.0	36.0	32.0	36.0
35	34.56306916276213	36.0	36.0	36.0	32.0	36.0
36	34.53479561008261	36.0	36.0	36.0	32.0	36.0
37	34.530020828860486	36.0	36.0	36.0	32.0	36.0
38	34.121331737373936	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	8.0
21	30.0
22	132.0
23	409.0
24	1146.0
25	2859.0
26	6206.0
27	12418.0
28	22243.0
29	35960.0
30	54984.0
31	80844.0
32	118509.0
33	192814.0
34	485787.0
35	1123549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.28943042575626	18.739094597843852	12.109077878956	26.86239709744389
2	15.661778222451108	20.00842883597401	37.44699819776332	26.882794743811566
3	18.088272645246573	23.512710375934503	29.130047771199667	29.268969207619257
4	11.898380463783084	16.23900320689126	36.645936004370654	35.216680324955
5	13.768577251383018	37.13551214113683	34.27288592481303	14.823024682667127
6	34.46463251720383	36.53208575159877	16.201536463887862	12.801745267309542
7	30.158941760333786	30.680951779558352	20.58272408776878	18.577382372339084
8	27.610517472678183	34.3779644241042	18.804762911557958	19.206755191659667
9	26.74672340990833	14.262025234152409	18.630687285207294	40.36056407073197
10	15.267526208157225	27.09001049635995	32.522063872501974	25.120399422980853
11	37.18959167625991	21.292933961209457	22.482433953958974	19.03504040857166
12	24.076028233445125	24.071678149951587	29.258521252262742	22.59377236434054
13	29.30358502674597	19.692388577159186	25.34332884916544	25.660697546929406
14	22.682081800870854	20.061565116032142	25.381554507486086	31.874798575610914
15	24.79537153064761	28.18206098604284	22.021152542753423	25.001414940556128
16	25.412858798689182	26.310703316996413	24.010172608796115	24.26626527551829
17	23.663138436380766	26.533292732724977	25.40480163001152	24.398767200882734
18	24.331317803132887	25.523329212465136	26.42963956669609	23.71571341770589
19	25.126958757172346	25.474215573326898	25.476554318047768	23.92227135145299
20	25.269060886412316	25.299838766938944	25.282812705371022	24.148287641277722
21	25.962360172038384	24.799393796234146	24.86656267029017	24.371683361437302
22	25.217000428925786	25.13205722066384	25.377297992094107	24.27364435831627
23	24.224435718311703	25.050762549142963	25.76772947221449	24.95707226033084
24	24.533385347015926	25.468041287263805	25.644850388161462	24.35372297755881
25	24.463982846702695	25.26215001838254	25.79660021198392	24.47726692293084
26	24.004559618840563	25.946420268880928	26.075098063612018	23.973922048666495
27	24.62336802164371	25.617148822954906	25.253146083813245	24.506337071588142
28	23.97498665746137	25.433427865394954	25.942479041339183	24.649106435804498
29	24.281596090367223	25.345070089840537	25.741721194499835	24.6316126252924
30	24.129951882666113	25.49886594268485	26.031351340732183	24.33983083391685
31	24.76749369357486	25.065636870591174	25.415793730199603	24.751075705634364
32	24.39231226545314	25.489464188906958	25.207926099408812	24.910297446231088
33	24.052960406455124	25.344368466424278	25.76852320900099	24.834147918119612
34	24.618983403799714	25.255355842348028	26.14136589240184	23.984294861450426
35	24.917500779971363	25.2426330710665	25.825495030401342	24.014371118560792
36	24.024801920015864	25.53792297952335	25.745463186053225	24.691811914407555
37	24.964977297804992	25.272428678810364	25.160215707103095	24.602378316281545
38	24.200312643540524	25.348449738949192	25.710955340245416	24.740282277264868
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	28.5
2	47.0
3	47.0
4	193.0
5	339.0
6	339.0
7	482.5
8	626.0
9	755.0
10	884.0
11	884.0
12	1224.5
13	1565.0
14	2715.0
15	3865.0
16	3865.0
17	5802.0
18	7739.0
19	7739.0
20	10060.0
21	12381.0
22	15691.5
23	19002.0
24	19002.0
25	23279.0
26	27556.0
27	27556.0
28	35408.5
29	43261.0
30	52198.0
31	61135.0
32	61135.0
33	74907.5
34	88680.0
35	88680.0
36	97558.5
37	106437.0
38	118002.0
39	129567.0
40	129567.0
41	136172.0
42	142777.0
43	155880.5
44	168984.0
45	168984.0
46	170745.0
47	172506.0
48	172506.0
49	181795.0
50	191084.0
51	192206.5
52	193329.0
53	193329.0
54	186669.0
55	180009.0
56	180009.0
57	169764.5
58	159520.0
59	143725.5
60	127931.0
61	127931.0
62	118121.0
63	108311.0
64	90562.0
65	72813.0
66	72813.0
67	60738.0
68	48663.0
69	48663.0
70	38903.5
71	29144.0
72	22616.0
73	16088.0
74	16088.0
75	12333.0
76	8578.0
77	8578.0
78	7649.0
79	6720.0
80	5303.5
81	3887.0
82	3887.0
83	3339.5
84	2792.0
85	2792.0
86	1866.5
87	941.0
88	741.0
89	541.0
90	541.0
91	330.0
92	119.0
93	78.0
94	37.0
95	37.0
96	29.5
97	22.0
98	22.0
99	15.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009214654200221807
2	0.0
3	0.0
4	1.4032468325210872E-4
5	9.354978883473916E-5
6	1.4032468325210872E-4
7	4.209740497563262E-4
8	0.00219842003761637
9	0.0038355413422243054
10	7.016234162605436E-4
11	0.005145238385910654
12	4.209740497563262E-4
13	3.2742426092158706E-4
14	0.0
15	0.0
16	4.677489441736958E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.870995776694783E-4
22	0.0
23	1.870995776694783E-4
24	0.0
25	4.677489441736958E-5
26	4.677489441736958E-5
27	1.4032468325210872E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	4.677489441736958E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2137899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.797210243304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.27266326165623	35.491797970409046
2	16.110530501913413	16.04518949069888
3	5.659097871749052	8.454218595207653
4	2.585423745234701	5.149875592379314
5	1.3321126362754268	3.3167746508184686
6	0.7577387412486407	2.2639965244473053
7	0.4907540618493314	1.7106728236966597
8	0.32147759522666386	1.280694991841117
9	0.22502241775546175	1.0084939781782782
>10	1.0742391344304847	9.337755456102363
>50	0.08179820918368846	2.8322512757017906
>100	0.07697646186963344	8.01457176517465
>500	0.009147597487782159	3.020317503465891
>1k	0.0030177641196807033	2.073389381878601
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3271	0.15300067963921588	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2230	0.10430801455073414	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4032468325210872E-4
2	0.0	0.0	0.0	0.0	1.4032468325210872E-4
3	0.0	0.0	0.0	0.0	1.4032468325210872E-4
4	0.0	0.0	0.0	0.0	1.4032468325210872E-4
5	0.0	0.0	0.0	0.0	1.4032468325210872E-4
6	0.0	0.0	0.0	0.0	1.870995776694783E-4
7	0.0	0.0	0.0	0.0	1.870995776694783E-4
8	0.0	0.0	0.0	0.0	1.870995776694783E-4
9	0.0	0.0	0.0	0.0	1.870995776694783E-4
10	0.0	0.0	0.0	0.0	1.870995776694783E-4
11	0.0	0.0	0.0	4.677489441736958E-5	1.870995776694783E-4
12	0.0	0.0	0.0	1.4032468325210872E-4	5.145238385910653E-4
13	0.0	0.0	0.0	1.4032468325210872E-4	6.548485218431741E-4
14	0.0	0.0	0.0	1.4032468325210872E-4	6.548485218431741E-4
15	0.0	0.0	0.0	1.4032468325210872E-4	7.483983106779132E-4
16	0.0	0.0	0.0	1.4032468325210872E-4	7.951732050952828E-4
17	0.0	0.0	0.0	1.870995776694783E-4	8.419480995126524E-4
18	0.0	0.0	0.0	2.3387447208684788E-4	8.419480995126524E-4
19	0.0	0.0	0.0	2.3387447208684788E-4	8.419480995126524E-4
20	0.0	0.0	0.0	2.8064936650421744E-4	9.822727827647612E-4
21	0.0	0.0	0.0	3.741991553389566E-4	9.822727827647612E-4
22	0.0	0.0	0.0	4.6774894417369575E-4	0.0010290476771821306
23	0.0	0.0	0.0	5.612987330084349E-4	0.0010290476771821306
24	0.0	0.0	0.0	7.483983106779132E-4	0.0010290476771821306
25	0.0	0.0	0.0	9.822727827647612E-4	0.0010290476771821306
26	0.0	0.0	0.0	0.0010758225715995003	0.0010758225715995003
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2345	0.0	19.31003	1
GGTATCA	980	0.0	19.266165	1
TCTAGCG	300	0.0	19.199509	28
TAGCGGC	325	0.0	17.722624	30
CTAGCGG	325	0.0	17.722624	29
TCCGTAT	55	0.0013647582	17.455324	8
TATACTG	260	0.0	17.231941	5
CCGTCGT	310	0.0	16.516867	9
CCGTATG	90	8.584082E-6	16.000713	9
TATGCGA	70	3.698977E-4	15.99959	12
GTCCTAC	675	0.0	15.882189	1
ATACCGT	355	0.0	15.775352	6
GTATAGG	285	0.0	15.719999	1
TAAACGC	340	0.0	15.529015	28
GTACCGT	125	5.140646E-8	15.360685	6
CGTCGTA	345	0.0	15.304314	10
TACGCTG	105	2.1854194E-6	15.239132	5
TACCGTC	370	0.0	15.135809	7
AACGAAT	215	0.0	14.883339	31
TACCGTA	65	0.0041619022	14.76989	7
>>END_MODULE