FastQCFastQC Report
Thu 2 Feb 2017
SRR4062887_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062887_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367323
Sequences flagged as poor quality0
Sequence length38
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35910.9776137078266267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24730.6732494289766772No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16640.4530072987534132No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA14960.40727098493696284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13610.37051858990588665No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT13170.35854003152538777No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13110.3569065917462288No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA12430.33839427424909413No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10960.29837499965970005No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA10730.29211348050625746No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC9430.25672228529114705No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT8270.2251424495607408No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG8190.22296452985519555No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC7620.20744685195318563No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA7450.20281877257890193No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA7370.20064085287335667No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC7190.19574053353587986No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT7170.19519605360949355No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG7140.1943793337199141No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT7070.192473653977562No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT6910.18811781456647148No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC6740.1834897351921878No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT6670.1815840554498357No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT6660.18131181548664255No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC6660.18131181548664255No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT6640.18076733556025623No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA6440.1753225362963931No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA6410.17450581640681362No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT6380.17368909651723413No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA6350.17287237662765467No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA6330.17232789670126836No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG6300.1715111768116889No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG6290.17123893684849573No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG6290.17123893684849573No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG6200.16878877717975732No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG5950.1619827780999284No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5910.16089381824715576No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA5550.1510931795722021No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC5460.1486430199034637No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTA5230.1423815007500211No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA5160.140475821007669No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCC5090.13857014126531692No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC5000.1361199815965785No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG4920.13394206189103322No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4900.13339758196464693No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCAT4820.13121966225910164No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTA4810.13094742229590853No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT4730.12876950259036324No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGG4690.1276805427375906No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTC4610.12550262303204537No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAA4470.12169126354734117No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCT4470.12169126354734117No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC4470.12169126354734117No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCG4470.12169126354734117No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT4430.12060230369456854No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGG4390.11951334384179592No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTG4310.11733542413625066No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT4260.11597422432028488No Hit
TATCAACGCAGAGTACATGGGAGAAATCGTAAATAGAT4180.11379630461473961No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTT4170.11352406465154646No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACA4130.11243510479877383No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4110.1118906248723875No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4100.11161838490919436No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4070.1108016650196149No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTC4060.11052942505642173No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4030.10971270516684226No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT3930.10699030553491069No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTA3890.10590134568213806No Hit
CTCTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACT3860.10508462579255859No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATT3840.10454014586617227No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC3790.10317894605020649No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCA3780.10290670608701334No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTAT3760.10236222616062701No Hit
GATAAAAGGAACTCGGCAAACAAGAACCCCGCCTGTTT3720.10127326630785441No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.10018430645508176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACTA200.003751858631.99714332
GTTTAGA1450.023.1892871
TCGGCAA903.45608E-1123.11534113
GATAACG350.00206168522.86132611
ATAACGA350.00206168522.86132612
CGATAAC350.002063318422.85821310
TCCGATA350.002064952622.85518
GGCAAAC957.2759576E-1121.89874515
CAGTTCC453.52608E-421.33723817
TAAATTC604.6986715E-621.33723616
TTCCGAA453.5325752E-421.33142920
AGATTAT1500.021.3285245
TATAGCC1600.021.0009849
CGGCAAA1001.4370016E-1020.80380414
CTCGGCA1001.4370016E-1020.80380412
ACTTGCT1001.4370016E-1020.7981418
TTATAGC1550.020.6433168
TGTAGGA2350.020.4264892
CTAGGAC555.823461E-520.3618183
TAACGAA400.00447303120.0036613
Produced by FastQC (version 0.11.5)