##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062887_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	367323
Sequences flagged as poor quality	0
Sequence length	38
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.961586941193445	32.0	32.0	32.0	32.0	32.0
2	30.657963155043383	32.0	32.0	32.0	32.0	32.0
3	30.76354053516932	32.0	32.0	32.0	32.0	32.0
4	30.79867854721866	32.0	32.0	32.0	32.0	32.0
5	30.79301323358461	32.0	32.0	32.0	32.0	32.0
6	34.340310843589975	36.0	36.0	36.0	32.0	36.0
7	34.155963552513725	36.0	36.0	36.0	32.0	36.0
8	34.1278520539144	36.0	36.0	36.0	32.0	36.0
9	34.22280935307617	36.0	36.0	36.0	32.0	36.0
10	34.03983687381405	36.0	36.0	36.0	32.0	36.0
11	34.37533451485477	36.0	36.0	36.0	32.0	36.0
12	34.1694911562848	36.0	36.0	36.0	32.0	36.0
13	34.27975923097655	36.0	36.0	36.0	32.0	36.0
14	34.188376442531506	36.0	36.0	36.0	32.0	36.0
15	34.18056043318823	36.0	36.0	36.0	32.0	36.0
16	34.15345894485235	36.0	36.0	36.0	32.0	36.0
17	34.077310704747596	36.0	36.0	36.0	32.0	36.0
18	34.1329674428228	36.0	36.0	36.0	32.0	36.0
19	34.078511282985275	36.0	36.0	36.0	32.0	36.0
20	34.00286396441279	36.0	36.0	36.0	32.0	36.0
21	33.95004941155332	36.0	36.0	36.0	32.0	36.0
22	33.95367564786305	36.0	36.0	36.0	32.0	36.0
23	33.946562017624814	36.0	36.0	36.0	32.0	36.0
24	33.920881621896804	36.0	36.0	36.0	32.0	36.0
25	33.92688723548485	36.0	36.0	36.0	32.0	36.0
26	33.90507809203344	36.0	36.0	36.0	32.0	36.0
27	33.894591408651245	36.0	36.0	36.0	32.0	36.0
28	33.89541901813935	36.0	36.0	36.0	32.0	36.0
29	33.83378116807279	36.0	36.0	36.0	32.0	36.0
30	33.75504392591806	36.0	36.0	36.0	27.0	36.0
31	33.760769132344016	36.0	36.0	36.0	27.0	36.0
32	33.71838409247447	36.0	36.0	36.0	27.0	36.0
33	33.65992328277837	36.0	36.0	36.0	27.0	36.0
34	33.59878635424408	36.0	36.0	36.0	27.0	36.0
35	33.51996471770077	36.0	36.0	36.0	21.0	36.0
36	33.45528050244608	36.0	36.0	36.0	21.0	36.0
37	33.42819807090762	36.0	36.0	36.0	21.0	36.0
38	32.81706563433273	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	1.0
11	0.0
12	4.0
13	3.0
14	92.0
15	187.0
16	220.0
17	254.0
18	318.0
19	445.0
20	567.0
21	796.0
22	1063.0
23	1467.0
24	2102.0
25	2906.0
26	4125.0
27	5974.0
28	8147.0
29	10868.0
30	14954.0
31	19425.0
32	25426.0
33	36971.0
34	78936.0
35	152070.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.829495398839896	21.57039120764098	12.995278904444154	22.60483448907497
2	13.876918383246256	22.92362423932052	41.46268380249846	21.73677357493476
3	16.618155184103117	29.528925822358122	31.84114009698666	22.0117788965521
4	9.27846278242262	19.129734041539503	42.13953766950802	29.45226550652985
5	9.906593960051291	41.774905328037335	36.05320686810718	12.265293843804193
6	25.60723289103294	42.79024942693332	20.072579371777348	11.529938310256396
7	25.10978076515764	35.998018093067955	22.921788180974236	15.970412960800168
8	25.639357294145192	34.816863678189705	21.384235085293014	18.15954394237209
9	27.00192201937265	16.04396142893701	22.291068871453383	34.66304768023696
10	15.304555675946444	27.305198164008686	34.546256418074606	22.84398974197026
11	31.122811507164073	25.413060582218726	26.41899820047425	17.045129710142955
12	22.845117432626314	29.650138708526967	30.592648856437354	16.912095002409362
13	25.68907258712279	23.22875058880548	29.309579626921956	21.772597197149775
14	21.137133397950407	25.496607603759408	28.48578242923886	24.88047656905133
15	22.790411432648295	30.43577713089108	27.578159676931836	19.195651759528793
16	21.54768938393555	29.436429129581278	28.434476956461367	20.58140453002181
17	18.91111782789954	28.930478516715276	29.604154242877257	22.554249412507932
18	19.50700237405528	27.277677346285365	33.315183063620324	19.90013721603903
19	21.247052801812153	29.13955425839509	30.57762361896879	19.035769320823963
20	20.898878851735084	28.18035295206669	31.43680132425089	19.483966871947334
21	23.792332677192153	26.47761088377589	28.4599195750601	21.27013686397186
22	21.38736349751294	29.267004445944877	28.97133413739685	20.374297919145334
23	20.95596443176311	28.8294935392356	30.026627170604474	20.187914858396816
24	20.509595068505085	30.7630312139232	29.435132544816923	19.2922411727548
25	21.168942560346355	28.747055860369496	30.6416522579679	19.44234932131625
26	18.608456978545775	28.94400771143127	32.9353269053253	19.512208404697656
27	20.358410838361177	30.63998823811287	29.706388447213083	19.295212476312866
28	18.95856531836287	28.88856823150495	31.762777667362734	20.390088782769446
29	19.57999689612642	28.800407315339942	31.1705142900706	20.449081498463038
30	20.421381045203585	28.514684903461866	31.70131439959054	19.362619651744005
31	20.254829092988118	28.396564163297533	30.52504049767904	20.82356624603531
32	17.841274162809693	30.628369180506397	30.95344405118432	20.576912605499594
33	19.159531167045564	29.86237220762602	30.688683483303613	20.289413142024802
34	19.08884968089046	29.430503822779848	32.860386852251196	18.6202596440785
35	20.90207343471335	30.20761575429186	29.761888554585923	19.12842225640886
36	19.325292019495194	29.470144580281538	30.54510305769597	20.659460342527296
37	21.202938325655353	29.976530423323638	28.343189466461922	20.477341784559087
38	19.26442401934093	31.197046588112297	29.68984819114412	19.848681201402652
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	491.0
1	393.0
2	295.0
3	295.0
4	666.5
5	1038.0
6	1038.0
7	1192.0
8	1346.0
9	1423.5
10	1501.0
11	1501.0
12	1800.0
13	2099.0
14	2600.5
15	3102.0
16	3102.0
17	4383.5
18	5665.0
19	5665.0
20	6556.5
21	7448.0
22	8389.5
23	9331.0
24	9331.0
25	10654.5
26	11978.0
27	11978.0
28	15399.0
29	18820.0
30	21832.5
31	24845.0
32	24845.0
33	27653.0
34	30461.0
35	30461.0
36	30658.5
37	30856.0
38	31915.5
39	32975.0
40	32975.0
41	31515.5
42	30056.0
43	31134.5
44	32213.0
45	32213.0
46	30844.0
47	29475.0
48	29475.0
49	26988.0
50	24501.0
51	19686.0
52	14871.0
53	14871.0
54	13866.5
55	12862.0
56	12862.0
57	11131.5
58	9401.0
59	8180.0
60	6959.0
61	6959.0
62	7214.0
63	7469.0
64	6538.0
65	5607.0
66	5607.0
67	5065.5
68	4524.0
69	4524.0
70	3625.0
71	2726.0
72	2106.5
73	1487.0
74	1487.0
75	1209.0
76	931.0
77	931.0
78	793.0
79	655.0
80	532.5
81	410.0
82	410.0
83	383.0
84	356.0
85	356.0
86	265.0
87	174.0
88	139.0
89	104.0
90	104.0
91	75.5
92	47.0
93	42.5
94	38.0
95	38.0
96	34.5
97	31.0
98	31.0
99	103.0
100	175.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12495814310565904
2	0.05907607201291506
3	0.01524543793881679
4	0.00272239963193157
5	5.44479926386314E-4
6	2.72239963193157E-4
7	0.0
8	2.72239963193157E-4
9	2.72239963193157E-4
10	2.72239963193157E-4
11	0.001088959852772628
12	0.0016334397791589418
13	0.014700958012430477
14	0.008439438858987866
15	0.025590556540156753
16	0.01088959852772628
17	0.022868156908225187
18	0.005172559300669983
19	0.006805999079828925
20	0.005717039227056297
21	0.006533759116635767
22	0.0059892791902494535
23	0.007894958932601552
24	0.013884238122851004
25	0.018512317497134674
26	0.020690237202679932
27	0.009528398711760495
28	0.0059892791902494535
29	0.01088959852772628
30	0.0019056797423520988
31	0.004900319337476825
32	0.00626151915344261
33	0.007622718969408395
34	0.013884238122851004
35	0.015789917865203104
36	0.01442871804923732
37	0.010617358564533122
38	0.005172559300669983
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	367323.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.914687135948967
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.14261250960143	20.975825187833692
2	11.96018602480727	6.677297019404882
3	3.8401834341796026	3.21592557329333
4	1.8856792829671165	2.1055258889107056
5	1.1095166862934107	1.5485905584997701
6	0.7709607621186638	1.2912677081181165
7	0.5971943054362334	1.1669344536935962
8	0.4783545783916227	1.0682494716680737
9	0.34353647787310265	0.8630741969652165
>10	2.9170641000563458	17.005851150190484
>50	0.46537208853163403	9.16180020730097
>100	0.4473963426226868	24.360659452767464
>500	0.0319568816159062	5.924435661206762
>1k	0.009986525504970686	4.634563470146923
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3591	0.9776137078266267	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2473	0.6732494289766772	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1664	0.4530072987534132	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	1496	0.40727098493696284	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1361	0.37051858990588665	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	1317	0.35854003152538777	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1311	0.3569065917462288	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	1243	0.33839427424909413	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1096	0.29837499965970005	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1073	0.29211348050625746	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC	943	0.25672228529114705	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT	827	0.2251424495607408	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG	819	0.22296452985519555	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC	762	0.20744685195318563	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA	745	0.20281877257890193	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA	737	0.20064085287335667	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC	719	0.19574053353587986	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT	717	0.19519605360949355	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG	714	0.1943793337199141	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT	707	0.192473653977562	No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT	691	0.18811781456647148	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC	674	0.1834897351921878	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT	667	0.1815840554498357	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT	666	0.18131181548664255	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC	666	0.18131181548664255	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT	664	0.18076733556025623	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA	644	0.1753225362963931	No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA	641	0.17450581640681362	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT	638	0.17368909651723413	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	635	0.17287237662765467	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA	633	0.17232789670126836	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG	630	0.1715111768116889	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG	629	0.17123893684849573	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG	629	0.17123893684849573	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	620	0.16878877717975732	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG	595	0.1619827780999284	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	591	0.16089381824715576	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	555	0.1510931795722021	No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	546	0.1486430199034637	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTA	523	0.1423815007500211	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	516	0.140475821007669	No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCC	509	0.13857014126531692	No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC	500	0.1361199815965785	No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG	492	0.13394206189103322	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	490	0.13339758196464693	No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCAT	482	0.13121966225910164	No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTA	481	0.13094742229590853	No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT	473	0.12876950259036324	No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGG	469	0.1276805427375906	No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTC	461	0.12550262303204537	No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAA	447	0.12169126354734117	No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCT	447	0.12169126354734117	No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC	447	0.12169126354734117	No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCG	447	0.12169126354734117	No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT	443	0.12060230369456854	No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGG	439	0.11951334384179592	No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTG	431	0.11733542413625066	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	426	0.11597422432028488	No Hit
TATCAACGCAGAGTACATGGGAGAAATCGTAAATAGAT	418	0.11379630461473961	No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTT	417	0.11352406465154646	No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACA	413	0.11243510479877383	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	411	0.1118906248723875	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	410	0.11161838490919436	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	407	0.1108016650196149	No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTC	406	0.11052942505642173	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	403	0.10971270516684226	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	393	0.10699030553491069	No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTA	389	0.10590134568213806	No Hit
CTCTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACT	386	0.10508462579255859	No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATT	384	0.10454014586617227	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	379	0.10317894605020649	No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCA	378	0.10290670608701334	No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTAT	376	0.10236222616062701	No Hit
GATAAAAGGAACTCGGCAAACAAGAACCCCGCCTGTTT	372	0.10127326630785441	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	368	0.10018430645508176	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.72239963193157E-4	0.0
4	0.0	0.0	0.0	2.72239963193157E-4	0.0
5	0.0	0.0	0.0	2.72239963193157E-4	0.0
6	0.0	0.0	0.0	2.72239963193157E-4	0.0
7	0.0	0.0	0.0	2.72239963193157E-4	0.0
8	0.0	0.0	0.0	2.72239963193157E-4	0.0
9	0.0	0.0	0.0	0.001361199815965785	0.0
10	0.0	0.0	0.0	0.001361199815965785	0.0
11	0.0	0.0	0.0	0.001361199815965785	0.0
12	0.0	0.0	0.0	0.0016334397791589418	0.0
13	0.0	0.0	0.0	0.0019056797423520988	0.0
14	0.0	0.0	0.0	0.002177919705545256	0.0
15	0.0	0.0	0.0	0.0024501596687384127	0.0
16	0.0	0.0	0.0	0.00272239963193157	0.0
17	0.0	0.0	0.0	0.00272239963193157	0.0
18	0.0	0.0	0.0	0.0032668795583178836	0.0
19	0.0	0.0	0.0	0.0032668795583178836	0.0
20	0.0	0.0	0.0	0.0046280793742836685	0.0
21	0.0	0.0	0.0	0.0046280793742836685	0.0
22	0.0	0.0	0.0	0.006533759116635767	0.0
23	0.0	0.0	0.0	0.008711678822181023	0.0
24	0.0	0.0	0.0	0.013067518233271534	0.0
25	0.0	0.0	0.0	0.014700958012430477	0.0
26	0.0	0.0	0.0	0.01687887771797573	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTACTA	20	0.0037518586	31.997143	32
GTTTAGA	145	0.0	23.189287	1
TCGGCAA	90	3.45608E-11	23.115341	13
GATAACG	35	0.002061685	22.861326	11
ATAACGA	35	0.002061685	22.861326	12
CGATAAC	35	0.0020633184	22.858213	10
TCCGATA	35	0.0020649526	22.8551	8
GGCAAAC	95	7.2759576E-11	21.898745	15
CAGTTCC	45	3.52608E-4	21.337238	17
TAAATTC	60	4.6986715E-6	21.337236	16
TTCCGAA	45	3.5325752E-4	21.331429	20
AGATTAT	150	0.0	21.328524	5
TATAGCC	160	0.0	21.000984	9
CGGCAAA	100	1.4370016E-10	20.803804	14
CTCGGCA	100	1.4370016E-10	20.803804	12
ACTTGCT	100	1.4370016E-10	20.798141	8
TTATAGC	155	0.0	20.643316	8
TGTAGGA	235	0.0	20.426489	2
CTAGGAC	55	5.823461E-5	20.361818	3
TAACGAA	40	0.004473031	20.00366	13
>>END_MODULE