FastQCFastQC Report
Thu 2 Feb 2017
SRR4062887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367323
Sequences flagged as poor quality0
Sequence length38
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40261.0960380918156498No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT30110.8197145291745956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18830.5126278506927145No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16070.4374896208514033No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC15660.4263277823604838No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA14180.38603626780789657No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT13650.3716075497586593No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA12850.3498283527032067No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12640.3441113134761504No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC9840.26788412378206644No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9700.26407276429736226No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT9370.25508884551198807No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG8250.22459796963435452No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA8220.223781249744775No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT8000.2177919705545256No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG7620.20744685195318563No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC7560.20581341217402668No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA7390.20118533279974302No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC7340.19982413298377721No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT7320.19927965305739093No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT7310.19900741309419773No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT7070.192473653977562No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA7040.1916569340879825No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT7020.1911124541615962No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT6990.1902957342720167No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA6980.19002349430882357No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT6930.1886622944928578No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC6870.18702885471369884No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG6850.18648437478731253No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG6840.18621213482411936No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC6680.18185629541302886No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA6650.18103957552344938No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG6500.17695597607555205No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG6290.17123893684849573No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA5930.1614382981735421No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5830.15871589854161053No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5790.1576269386888379No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC5780.15735469872564473No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG5580.1519098994617816No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA5580.1519098994617816No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC5370.1461928602347253No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC5310.14455942045556636No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTA5240.14265374071321424No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT5230.1423815007500211No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA5160.140475821007669No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTG5060.13775342137573743No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCAT4950.1347587817806127No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG4940.13448654181741956No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4880.1328531020382606No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTA4850.13203638214868113No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACA4850.13203638214868113No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.13121966225910164No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCT4810.13094742229590853No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA4760.12958622247994273No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.12795278270078378No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT4680.12740830277439746No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4650.126591582884818No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA4610.12550262303204537No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCG4570.12441366317927274No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTAT4520.12305246336330695No Hit
ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTT4520.12305246336330695No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTT4490.1222357434737275No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATT4390.11951334384179592No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCA4380.11924110387860276No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTC4330.11787990406263699No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCC4320.11760766409944381No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAA4290.11679094420986434No Hit
TATCAACGCAGAGTACATGGGAGAAATCGTAAATAGAT4210.11461302450431908No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGA4190.11406854457793278No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT4040.10998494513003543No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTA4030.10971270516684226No Hit
GATAAAAGGAACTCGGCAAACAAGAACCCCGCCTGTTT4010.10916822524045595No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGG4010.10916822524045595No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC3990.10862374531406963No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGG3910.10644582560852438No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGC3880.10562910571894492No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG3800.10345118601339964No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTA3760.10236222616062701No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCA3720.10127326630785441No Hit
CAATTAGGGTTTACGACCTCGATGTTGGATCAGGACAT3720.10127326630785441No Hit
CTATAAATAATCCACCTATAACTTCTCTGTTAACCCAA3710.10100102634466124No Hit
CAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTCCCTA3700.10072878638146808No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGA3700.10072878638146808No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC3680.10018430645508176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATGT200.003750295831.9998666
TATAGGG356.419118E-527.4284552
TTAGAGT308.4061193E-426.6665534
TAGGGCG308.4061193E-426.66655331
GTATAGG509.635096E-725.6033761
TAGAGTG509.646537E-725.5998925
AGGGGTC401.5907203E-423.99995
TGCTAAA401.5907203E-423.999911
TCCCCGC350.002062298822.8601571
GCCCCTT350.002063932122.8570466
CCCTCGA350.002063932122.85704626
CCGCCCC350.002063932122.8570464
GGGGTCC502.7910119E-522.3999066
CATCGTT502.7910119E-522.39990628
GCTAAAA502.7910119E-522.39990612
TAAGAGG502.7910119E-522.3999064
CCTACAG2850.020.7718413
GTCCTAA1950.020.5155281
AACTTGC555.8196514E-520.363557
ATCGTTT555.8196514E-520.3635529
Produced by FastQC (version 0.11.5)