##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062887_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	367323
Sequences flagged as poor quality	0
Sequence length	38
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.997990869071636	32.0	32.0	32.0	32.0	32.0
2	31.32681863101412	32.0	32.0	32.0	32.0	32.0
3	31.408575014360657	32.0	32.0	32.0	32.0	32.0
4	31.49799223027145	32.0	32.0	32.0	32.0	32.0
5	31.420542683142628	32.0	32.0	32.0	32.0	32.0
6	34.879699882664575	36.0	36.0	36.0	32.0	36.0
7	34.923339948764436	36.0	36.0	36.0	36.0	36.0
8	34.89268845130852	36.0	36.0	36.0	32.0	36.0
9	35.004647136171705	36.0	36.0	36.0	36.0	36.0
10	34.86429926794674	36.0	36.0	36.0	32.0	36.0
11	35.0395428546538	36.0	36.0	36.0	36.0	36.0
12	34.91310100374874	36.0	36.0	36.0	32.0	36.0
13	35.00378958028765	36.0	36.0	36.0	36.0	36.0
14	34.93202168119067	36.0	36.0	36.0	32.0	36.0
15	34.911004756032156	36.0	36.0	36.0	32.0	36.0
16	34.90500458724338	36.0	36.0	36.0	32.0	36.0
17	34.887453276816316	36.0	36.0	36.0	32.0	36.0
18	34.861217511563396	36.0	36.0	36.0	32.0	36.0
19	34.849225341184734	36.0	36.0	36.0	32.0	36.0
20	34.802176830745694	36.0	36.0	36.0	32.0	36.0
21	34.78074882324276	36.0	36.0	36.0	32.0	36.0
22	34.776755062982716	36.0	36.0	36.0	32.0	36.0
23	34.73525752539318	36.0	36.0	36.0	32.0	36.0
24	34.69221638721235	36.0	36.0	36.0	32.0	36.0
25	34.690923247387175	36.0	36.0	36.0	32.0	36.0
26	34.628678302202694	36.0	36.0	36.0	32.0	36.0
27	34.602004230609026	36.0	36.0	36.0	32.0	36.0
28	34.5634741086183	36.0	36.0	36.0	32.0	36.0
29	34.4998380172219	36.0	36.0	36.0	32.0	36.0
30	34.455035486479204	36.0	36.0	36.0	32.0	36.0
31	34.41829670344629	36.0	36.0	36.0	32.0	36.0
32	34.36093846560112	36.0	36.0	36.0	32.0	36.0
33	34.3511405493258	36.0	36.0	36.0	32.0	36.0
34	34.27577091551577	36.0	36.0	36.0	32.0	36.0
35	34.186293806813076	36.0	36.0	36.0	32.0	36.0
36	34.12063225009052	36.0	36.0	36.0	32.0	36.0
37	34.09467144720042	36.0	36.0	36.0	32.0	36.0
38	33.68882427727096	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	0.0
21	8.0
22	23.0
23	99.0
24	262.0
25	568.0
26	1290.0
27	2498.0
28	4494.0
29	7390.0
30	11123.0
31	16116.0
32	22474.0
33	35045.0
34	82715.0
35	183215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.23322741987742	22.841911234177644	13.827015282576555	23.097846063368372
2	13.350375555029226	24.071729785502132	40.462209009509344	22.1156856499593
3	15.77140554770597	29.79557501163826	32.45345377229305	21.97956566836272
4	8.914546132674147	20.0639222914018	42.11166799611239	28.909863579811663
5	9.61851013957743	42.15336366086523	36.01054113137484	12.2175850681825
6	25.638198533715556	43.2488572727545	19.445556090960817	11.667388102569129
7	24.478656212566698	36.153490144832844	23.178155286943262	16.189698355657193
8	25.458663769986146	35.14430314959558	21.308025492966564	18.08900758745171
9	26.498781699631106	15.749424880552116	22.44912404884091	35.30266937097586
10	14.949716594340599	27.635454837497754	34.847189628605186	22.56763893955646
11	31.072391168223028	25.155046146306937	26.608042253137675	17.16452043233236
12	22.376280211912164	29.89230157736264	30.728625021098654	17.002793189626537
13	25.928275268770367	22.900405911995232	29.585839088971227	21.585479730263177
14	20.934436449664194	25.512695910683515	28.662784524791533	24.89008311486076
15	22.68575613288577	30.610933701401766	27.351949102016484	19.351361063695986
16	21.527919569425276	29.217065089852802	28.456971112617506	20.79804422810442
17	18.738276666584994	29.071144469581267	29.829060527111018	22.36151833672272
18	19.43983905173376	27.135790571241113	33.47843723371528	19.945933143309837
19	21.382271189116935	28.818233543774824	30.872284066067195	18.927211201041043
20	20.75993063325738	28.388911121819216	31.440448869251313	19.41070937567209
21	23.52323029930143	26.38992491601374	28.82947386761479	21.257370917070038
22	21.096691467727315	29.004990158525327	29.496111052125784	20.40220732162157
23	20.834796623135496	28.02955437040425	30.54341819052986	20.592230815930392
24	20.505386267671778	30.445684043743515	29.744121658594754	19.304808029989953
25	21.148689300697207	28.180919789939647	31.037261483762247	19.633129425600902
26	18.658782597332593	28.648083566779103	33.21763134897624	19.475502486912063
27	20.585208618051738	29.944299551891802	30.201839258198525	19.268652571857935
28	18.946267998464567	28.290360255143295	32.24028988111281	20.523081865279334
29	19.63394614549048	28.20896050614854	31.650073640910044	20.507019707450937
30	20.468906112603893	27.82891351753089	32.098997340215554	19.603183029649653
31	20.20973366764401	27.71675065269531	30.956133974730687	21.117381704929993
32	17.848868706832953	29.7384046193677	31.517492778834978	20.895233894964377
33	19.053258303999478	29.413350103315068	31.13662907032775	20.396762522357704
34	19.064420142490395	28.773586189811144	33.4141886024017	18.747805065296756
35	21.12391546404663	29.60337359762389	30.045491297849576	19.227219640479902
36	19.397369617475626	28.689464041184458	31.031544444535193	20.88162189680472
37	21.399694546761296	29.074955829065974	28.8470909798733	20.678258644299433
38	19.42159897419982	30.32644293986492	30.150303683678942	20.101654402256326
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	271.0
1	219.0
2	167.0
3	167.0
4	530.5
5	894.0
6	894.0
7	1146.5
8	1399.0
9	1522.5
10	1646.0
11	1646.0
12	1875.5
13	2105.0
14	2735.0
15	3365.0
16	3365.0
17	4777.0
18	6189.0
19	6189.0
20	6930.0
21	7671.0
22	8683.0
23	9695.0
24	9695.0
25	10807.0
26	11919.0
27	11919.0
28	15427.5
29	18936.0
30	21782.0
31	24628.0
32	24628.0
33	27544.5
34	30461.0
35	30461.0
36	30305.0
37	30149.0
38	31559.5
39	32970.0
40	32970.0
41	31078.0
42	29186.0
43	30632.5
44	32079.0
45	32079.0
46	31102.5
47	30126.0
48	30126.0
49	27447.5
50	24769.0
51	20025.5
52	15282.0
53	15282.0
54	14089.5
55	12897.0
56	12897.0
57	11225.5
58	9554.0
59	8293.5
60	7033.0
61	7033.0
62	7253.0
63	7473.0
64	6462.0
65	5451.0
66	5451.0
67	4911.0
68	4371.0
69	4371.0
70	3583.5
71	2796.0
72	2095.0
73	1394.0
74	1394.0
75	1140.5
76	887.0
77	887.0
78	767.5
79	648.0
80	512.0
81	376.0
82	376.0
83	348.0
84	320.0
85	320.0
86	229.0
87	138.0
88	101.5
89	65.0
90	65.0
91	35.5
92	6.0
93	4.5
94	3.0
95	3.0
96	2.0
97	1.0
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.011434078454112593
2	0.0
3	0.0
4	5.44479926386314E-4
5	0.0
6	0.0
7	8.167198895794709E-4
8	0.0016334397791589418
9	0.002177919705545256
10	0.001361199815965785
11	0.003539119521511041
12	2.72239963193157E-4
13	5.44479926386314E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.72239963193157E-4
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	2.72239963193157E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	367323.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.4402174652826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.28053665015804	19.63993542468073
2	12.134347875330773	6.4166959324627095
3	4.19476735206598	3.327316830146765
4	1.9213146487371424	2.031999085273724
5	1.1748231587401283	1.5531289900169605
6	0.7917958011140742	1.2561151901732264
7	0.6455864334181073	1.194861198454766
8	0.4643691889498667	0.9822417872009104
9	0.3778791404536609	0.8992085984269975
>10	2.964343447864005	16.532316244830845
>50	0.5107031435014054	9.402896088728449
>100	0.4921695616807899	25.145716440299136
>500	0.038096807075709685	6.873786830664021
>1k	0.00926679091030776	4.743781358640761
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4026	1.0960380918156498	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3011	0.8197145291745956	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1883	0.5126278506927145	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1607	0.4374896208514033	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1566	0.4263277823604838	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	1418	0.38603626780789657	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	1365	0.3716075497586593	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	1285	0.3498283527032067	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1264	0.3441113134761504	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC	984	0.26788412378206644	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	970	0.26407276429736226	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT	937	0.25508884551198807	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG	825	0.22459796963435452	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA	822	0.223781249744775	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT	800	0.2177919705545256	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG	762	0.20744685195318563	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC	756	0.20581341217402668	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA	739	0.20118533279974302	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC	734	0.19982413298377721	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT	732	0.19927965305739093	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT	731	0.19900741309419773	No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT	707	0.192473653977562	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA	704	0.1916569340879825	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT	702	0.1911124541615962	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT	699	0.1902957342720167	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	698	0.19002349430882357	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT	693	0.1886622944928578	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC	687	0.18702885471369884	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG	685	0.18648437478731253	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG	684	0.18621213482411936	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC	668	0.18185629541302886	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA	665	0.18103957552344938	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG	650	0.17695597607555205	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	629	0.17123893684849573	No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA	593	0.1614382981735421	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	583	0.15871589854161053	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	579	0.1576269386888379	No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC	578	0.15735469872564473	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG	558	0.1519098994617816	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	558	0.1519098994617816	No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	537	0.1461928602347253	No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC	531	0.14455942045556636	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTA	524	0.14265374071321424	No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT	523	0.1423815007500211	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	516	0.140475821007669	No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTG	506	0.13775342137573743	No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCAT	495	0.1347587817806127	No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG	494	0.13448654181741956	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	488	0.1328531020382606	No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTA	485	0.13203638214868113	No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACA	485	0.13203638214868113	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	482	0.13121966225910164	No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCT	481	0.13094742229590853	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	476	0.12958622247994273	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	470	0.12795278270078378	No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT	468	0.12740830277439746	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	465	0.126591582884818	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	461	0.12550262303204537	No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCG	457	0.12441366317927274	No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTAT	452	0.12305246336330695	No Hit
ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTT	452	0.12305246336330695	No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTT	449	0.1222357434737275	No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATT	439	0.11951334384179592	No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCA	438	0.11924110387860276	No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTC	433	0.11787990406263699	No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCC	432	0.11760766409944381	No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAA	429	0.11679094420986434	No Hit
TATCAACGCAGAGTACATGGGAGAAATCGTAAATAGAT	421	0.11461302450431908	No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGA	419	0.11406854457793278	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	404	0.10998494513003543	No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTA	403	0.10971270516684226	No Hit
GATAAAAGGAACTCGGCAAACAAGAACCCCGCCTGTTT	401	0.10916822524045595	No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGG	401	0.10916822524045595	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	399	0.10862374531406963	No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGG	391	0.10644582560852438	No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGC	388	0.10562910571894492	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	380	0.10345118601339964	No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTA	376	0.10236222616062701	No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCA	372	0.10127326630785441	No Hit
CAATTAGGGTTTACGACCTCGATGTTGGATCAGGACAT	372	0.10127326630785441	No Hit
CTATAAATAATCCACCTATAACTTCTCTGTTAACCCAA	371	0.10100102634466124	No Hit
CAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTCCCTA	370	0.10072878638146808	No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGA	370	0.10072878638146808	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	368	0.10018430645508176	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.72239963193157E-4	0.0
9	0.0	0.0	0.0	2.72239963193157E-4	0.0
10	0.0	0.0	0.0	5.44479926386314E-4	0.0
11	0.0	0.0	0.0	5.44479926386314E-4	0.0
12	0.0	0.0	0.0	8.167198895794709E-4	0.0
13	0.0	0.0	0.0	0.001088959852772628	0.0
14	0.0	0.0	0.0	0.001361199815965785	0.0
15	0.0	0.0	0.0	0.001361199815965785	0.0
16	0.0	0.0	0.0	0.0016334397791589418	0.0
17	0.0	0.0	0.0	0.0016334397791589418	0.0
18	0.0	0.0	0.0	0.0019056797423520988	0.0
19	0.0	0.0	0.0	0.0019056797423520988	0.0
20	0.0	0.0	0.0	0.0032668795583178836	0.0
21	0.0	0.0	0.0	0.0032668795583178836	0.0
22	0.0	0.0	0.0	0.005172559300669982	0.0
23	0.0	0.0	0.0	0.007078239043022082	0.0
24	0.0	0.0	0.0	0.011161838490919436	0.0
25	0.0	0.0	0.0	0.013067518233271534	0.0
26	0.0	0.0	0.0	0.01524543793881679	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGATGT	20	0.0037502958	31.999866	6
TATAGGG	35	6.419118E-5	27.428455	2
TTAGAGT	30	8.4061193E-4	26.666553	4
TAGGGCG	30	8.4061193E-4	26.666553	31
GTATAGG	50	9.635096E-7	25.603376	1
TAGAGTG	50	9.646537E-7	25.599892	5
AGGGGTC	40	1.5907203E-4	23.9999	5
TGCTAAA	40	1.5907203E-4	23.9999	11
TCCCCGC	35	0.0020622988	22.860157	1
GCCCCTT	35	0.0020639321	22.857046	6
CCCTCGA	35	0.0020639321	22.857046	26
CCGCCCC	35	0.0020639321	22.857046	4
GGGGTCC	50	2.7910119E-5	22.399906	6
CATCGTT	50	2.7910119E-5	22.399906	28
GCTAAAA	50	2.7910119E-5	22.399906	12
TAAGAGG	50	2.7910119E-5	22.399906	4
CCTACAG	285	0.0	20.771841	3
GTCCTAA	195	0.0	20.515528	1
AACTTGC	55	5.8196514E-5	20.36355	7
ATCGTTT	55	5.8196514E-5	20.36355	29
>>END_MODULE