FastQCFastQC Report
Thu 2 Feb 2017
SRR4062886_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062886_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512475
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT23830.46499829259963904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17370.33894336309088247No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10490.2046929118493585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT9490.18517976486657886No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC8550.166837406702766No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8280.16156885701741547No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8020.15649543880189276No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT7980.15571491292258158No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC7350.14342163032343042No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG7340.1432264988536026No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA7240.14127518415532464No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC7030.13717742328894092No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC6840.13346992536221278No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA6500.12683545538806773No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG6150.12000585394409483No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG6070.11844480218547247No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC6050.11805453924581687No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT5780.11278598956046637No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5180.10107810137079858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATG453.843379E-728.4486083
CGTGATT451.2367975E-524.88767628
CCGTGAT451.2367975E-524.88767627
GTACGCC350.002065573822.8560284
GGTTCCG555.8274047E-520.3626428
CGACGAA400.00448141419.99902532
GGACCGT400.00448141419.9990256
TAGGACG801.2627243E-719.9990254
GCTCGCT400.00448141419.99902511
TCCTATA507.1625726E-419.2103082
GACGTGA601.1370451E-418.6657567
GTGCTAT450.00880654617.7907961
CTGAGCG450.00884625717.7769128
TACATTG450.00884625717.776915
GGACGTG904.3572982E-717.776916
AGGATAA450.00884625717.776915
GTAGTAC550.001361911117.45713
GTTATTC1201.6480044E-917.3358693
TCCGTGA652.098326E-417.22992926
AGGACGT957.6758624E-716.8412845