##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062886_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	512475
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.094586077369627	32.0	32.0	32.0	32.0	32.0
2	30.696502268403336	32.0	32.0	32.0	32.0	32.0
3	30.772455241719108	32.0	32.0	32.0	32.0	32.0
4	30.82635640762964	32.0	32.0	32.0	32.0	32.0
5	30.759697546221766	32.0	32.0	32.0	32.0	32.0
6	34.38461193228938	36.0	36.0	36.0	32.0	36.0
7	34.262992341089806	36.0	36.0	36.0	32.0	36.0
8	34.234497292550856	36.0	36.0	36.0	32.0	36.0
9	34.39553539197034	36.0	36.0	36.0	32.0	36.0
10	34.10193863115274	36.0	36.0	36.0	32.0	36.0
11	34.432825015854434	36.0	36.0	36.0	32.0	36.0
12	34.216320796136394	36.0	36.0	36.0	32.0	36.0
13	34.30575540270257	36.0	36.0	36.0	32.0	36.0
14	34.201533733352846	36.0	36.0	36.0	32.0	36.0
15	34.14928337967706	36.0	36.0	36.0	32.0	36.0
16	34.171826918386266	36.0	36.0	36.0	32.0	36.0
17	34.09957363773842	36.0	36.0	36.0	32.0	36.0
18	34.12828333089419	36.0	36.0	36.0	32.0	36.0
19	34.10394068003317	36.0	36.0	36.0	32.0	36.0
20	34.06089077515976	36.0	36.0	36.0	32.0	36.0
21	34.0424918288697	36.0	36.0	36.0	32.0	36.0
22	34.01322015708083	36.0	36.0	36.0	32.0	36.0
23	33.954760720035125	36.0	36.0	36.0	32.0	36.0
24	33.964489975120735	36.0	36.0	36.0	32.0	36.0
25	33.912618176496416	36.0	36.0	36.0	32.0	36.0
26	33.888685301722035	36.0	36.0	36.0	32.0	36.0
27	33.89409824869506	36.0	36.0	36.0	32.0	36.0
28	33.86116786184692	36.0	36.0	36.0	32.0	36.0
29	33.8242509390702	36.0	36.0	36.0	32.0	36.0
30	33.77897848675545	36.0	36.0	36.0	32.0	36.0
31	33.80684911459095	36.0	36.0	36.0	32.0	36.0
32	33.74745890043417	36.0	36.0	36.0	32.0	36.0
33	33.69774915849553	36.0	36.0	36.0	27.0	36.0
34	33.69500365871506	36.0	36.0	36.0	27.0	36.0
35	33.644638275037806	36.0	36.0	36.0	27.0	36.0
36	33.5994321674228	36.0	36.0	36.0	27.0	36.0
37	33.57256646665691	36.0	36.0	36.0	27.0	36.0
38	32.971179081906435	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	0.0
11	8.0
12	7.0
13	5.0
14	102.0
15	204.0
16	277.0
17	289.0
18	402.0
19	478.0
20	618.0
21	822.0
22	1244.0
23	1855.0
24	2705.0
25	3967.0
26	5717.0
27	8217.0
28	11175.0
29	15100.0
30	20268.0
31	26782.0
32	35977.0
33	52248.0
34	111004.0
35	213002.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.04545214787088	17.7850624854702	11.70589143137343	24.463593935285488
2	15.980039592826436	20.759682476464917	38.24417138149289	25.016106549215756
3	18.88155558070824	25.686635728866218	28.754200606526602	26.677608083898946
4	11.9544006993662	16.248165724811887	36.96708451715633	34.83034905866558
5	13.703524471485382	37.852261773280205	33.72014970515577	14.724064050078638
6	32.14972857159582	36.72244835835574	17.96891159356377	13.158911476484661
7	28.846870579052634	31.400360993219184	21.614127518415533	18.13864090931265
8	27.93346016878872	32.886677398897504	20.268110639543394	18.911751792770378
9	27.608566271525444	14.326747646226645	19.19352163520172	38.8711644470462
10	15.908124119467523	27.164304920835008	32.0203561546537	24.90721480504377
11	36.693536584651945	22.118801332373277	22.540680509455203	18.646981573519568
12	24.00569013358935	24.959704643820274	29.609378963677592	21.425226258912787
13	29.22877561933311	20.360027009832045	25.794779920609827	24.61641745022502
14	23.750822052271793	20.369138059898095	25.728626153068575	30.15141373476154
15	24.65845128149079	27.458955602570008	23.99623710897551	23.88635600696369
16	24.77855382327629	26.41351841335449	25.24964531533272	23.5582824480365
17	22.921724603626885	26.278308183514316	26.43677936595914	24.363187846899663
18	24.232560771664023	25.244559967684715	27.75367791261994	22.769201348031324
19	24.132682332829862	26.139153309057416	26.875041468107607	22.85312289000511
20	24.83534882631765	24.518438004320448	26.844922499302363	23.801290670059537
21	26.484650030052066	24.664743854938294	25.39457970041605	23.456026414593595
22	25.139479471783094	25.067471182048273	26.292197540790063	23.50085180537857
23	23.4796150153385	25.06322740791045	26.65835343340437	24.798804143346683
24	23.345875226378567	26.33231593080622	26.579380815587335	23.742428027227877
25	24.187643199231847	25.127927899796642	26.509471153283187	24.174957747688318
26	23.330548963277543	26.13742817886585	27.312328253809643	23.219694604046964
27	24.627904026852185	25.540019709816853	26.31533755500698	23.516738708323984
28	23.290603711213063	25.790959845603545	27.27482061559537	23.643615827588025
29	22.966722870123178	26.026883986698518	27.23467284904688	23.771720294131423
30	23.39167935058346	26.151504507668893	27.63747414432346	22.81934199742419
31	23.408332520245878	26.24158454483364	26.169577519758025	24.180505415162454
32	23.5619892124798	26.156809329409654	26.13436785287758	24.146833605232963
33	23.18222621184919	25.738232768714386	26.674732651627508	24.404808367808915
34	24.032473678561324	26.272259789428492	26.749412097616187	22.945854434393997
35	24.76688225260635	25.50126658365892	26.928466321364862	22.80338484236986
36	23.04185507336884	26.537486509852442	26.497674718146175	23.922983698632542
37	24.1436778917459	26.696251541692035	26.26418746975161	22.895883096810454
38	23.029638360484267	26.392737547907675	26.473526863423125	24.104097228184933
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	607.0
1	427.5
2	248.0
3	248.0
4	580.0
5	912.0
6	912.0
7	998.0
8	1084.0
9	1036.0
10	988.0
11	988.0
12	1267.5
13	1547.0
14	1889.5
15	2232.0
16	2232.0
17	3122.5
18	4013.0
19	4013.0
20	4734.5
21	5456.0
22	5609.0
23	5762.0
24	5762.0
25	6662.0
26	7562.0
27	7562.0
28	9555.5
29	11549.0
30	13695.5
31	15842.0
32	15842.0
33	18235.0
34	20628.0
35	20628.0
36	22526.0
37	24424.0
38	27613.0
39	30802.0
40	30802.0
41	32239.0
42	33676.0
43	37520.0
44	41364.0
45	41364.0
46	43323.0
47	45282.0
48	45282.0
49	45697.5
50	46113.0
51	45092.0
52	44071.0
53	44071.0
54	40924.5
55	37778.0
56	37778.0
57	36429.0
58	35080.0
59	31207.5
60	27335.0
61	27335.0
62	25756.0
63	24177.0
64	20263.5
65	16350.0
66	16350.0
67	13979.5
68	11609.0
69	11609.0
70	9262.0
71	6915.0
72	5199.0
73	3483.0
74	3483.0
75	2697.0
76	1911.0
77	1911.0
78	1646.5
79	1382.0
80	1096.0
81	810.0
82	810.0
83	752.0
84	694.0
85	694.0
86	462.5
87	231.0
88	191.5
89	152.0
90	152.0
91	106.0
92	60.0
93	58.5
94	57.0
95	57.0
96	59.0
97	61.0
98	61.0
99	144.5
100	228.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1162983560173667
2	0.05092931362505488
3	0.009561442021562026
4	0.0021464461681057613
5	1.9513146982779649E-4
6	1.9513146982779649E-4
7	0.0
8	0.0
9	0.0
10	1.9513146982779649E-4
11	0.001170788818966779
12	0.0017561832284501682
13	0.013464071418117958
14	0.007414995853456266
15	0.020098541392263036
16	0.009366310551734232
17	0.01541538611639592
18	0.004683155275867116
19	0.006439338504317283
20	0.005463681155178301
21	0.006049075564661691
22	0.00663446997414508
23	0.007610127323284063
24	0.011512756719839992
25	0.015025123176740329
26	0.01775696375432948
27	0.007805258793111859
28	0.005463681155178301
29	0.00995170496121762
30	0.002926972047416947
31	0.004878286745694912
32	0.006049075564661691
33	0.006829601443972876
34	0.011707888189667788
35	0.014244597297429143
36	0.012488414068978975
37	0.00995170496121762
38	0.006049075564661691
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	512475.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.07652599433573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.82851723586109	56.504982589491426
2	9.447885581880037	12.296711433214519
3	1.7767850138757317	3.4688098842549064
4	0.555610230192924	1.4462873435147472
5	0.26369975839221665	0.8580332090855571
6	0.1726473941144327	0.6741175578565324
7	0.10762316355679331	0.49026191205574143
8	0.08755819718498045	0.4558386636100443
9	0.06425117404181203	0.37631188779287134
>10	0.5321689956559937	7.193988342506051
>50	0.08825314427867233	4.086498314355365
>100	0.06897600566691063	8.804553371871354
>500	0.005120489503657112	2.330409524871509
>1k	9.036157947630199E-4	1.0131959655194573
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2383	0.46499829259963904	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1737	0.33894336309088247	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1049	0.2046929118493585	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	949	0.18517976486657886	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	855	0.166837406702766	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	828	0.16156885701741547	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	802	0.15649543880189276	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	798	0.15571491292258158	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	735	0.14342163032343042	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	734	0.1432264988536026	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	724	0.14127518415532464	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	703	0.13717742328894092	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	684	0.13346992536221278	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	650	0.12683545538806773	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	615	0.12000585394409483	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	607	0.11844480218547247	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	605	0.11805453924581687	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	578	0.11278598956046637	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	518	0.10107810137079858	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	1.9513146982779649E-4	0.0
19	0.0	0.0	0.0	1.9513146982779649E-4	0.0
20	0.0	0.0	0.0	3.9026293965559297E-4	0.0
21	0.0	0.0	0.0	0.0011707888189667788	0.0
22	0.0	0.0	0.0	0.0017561832284501682	0.0
23	0.0	0.0	0.0	0.0025367091077613544	0.0
24	0.0	0.0	0.0	0.004878286745694912	0.0
25	0.0	0.0	0.0	0.006829601443972877	0.0
26	0.0	0.0	0.0	0.009561442021562028	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAATG	45	3.843379E-7	28.448608	3
CGTGATT	45	1.2367975E-5	24.887676	28
CCGTGAT	45	1.2367975E-5	24.887676	27
GTACGCC	35	0.0020655738	22.856028	4
GGTTCCG	55	5.8274047E-5	20.362642	8
CGACGAA	40	0.004481414	19.999025	32
GGACCGT	40	0.004481414	19.999025	6
TAGGACG	80	1.2627243E-7	19.999025	4
GCTCGCT	40	0.004481414	19.999025	11
TCCTATA	50	7.1625726E-4	19.210308	2
GACGTGA	60	1.1370451E-4	18.665756	7
GTGCTAT	45	0.008806546	17.790796	1
CTGAGCG	45	0.008846257	17.77691	28
TACATTG	45	0.008846257	17.77691	5
GGACGTG	90	4.3572982E-7	17.77691	6
AGGATAA	45	0.008846257	17.77691	5
GTAGTAC	55	0.0013619111	17.4571	3
GTTATTC	120	1.6480044E-9	17.335869	3
TCCGTGA	65	2.098326E-4	17.229929	26
AGGACGT	95	7.6758624E-7	16.841284	5
>>END_MODULE