FastQCFastQC Report
Thu 2 Feb 2017
SRR4062886_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062886_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512475
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27460.5358310161471291No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21980.4288989706814967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12470.2433289428752622No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11230.21913264061661544No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10950.21366895946143713No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9660.1884969998536514No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9150.1785452948924338No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT8430.16449582906483243No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC7630.14888531147860873No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG7510.14654373384067515No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA7250.14147031562515244No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC7250.14147031562515244No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC7040.13737255475876872No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG6940.13542124006049075No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC6640.12956729596565686No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG5910.11532269866822771No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT5640.11005414898287721No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT5570.10868822869408264No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA5540.10810283428459924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG1750.023.7714329
TCTAGCG1700.023.52941128
TAGACTG552.214545E-623.2727285
CGCGGTC756.3850166E-821.33333210
GAACCGC851.053013E-820.7058836
TAGCGGC1950.020.51282130
GGATAGG400.00448014920.01
GCCGCGC400.00448014920.08
AGTGCGG400.00448014920.010
CAGTGCG400.00448014920.09
AGCGGCG2100.019.80952531
GCATAAA507.1884954E-419.21
CGAATGT507.1884954E-419.223
AACCGCG852.3895518E-718.823537
TCGCGTA1055.9535523E-918.2857159
CGGTCCA2200.018.18181810
CGCGTAA1159.367795E-1018.08695610
TACCGTC2050.017.951227
AGACGTT450.00884377117.7777795
ACAATAC450.00884377117.7777793
Produced by FastQC (version 0.11.5)