##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062886_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	512475
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10414556807649	32.0	32.0	32.0	32.0	32.0
2	31.267125225620763	32.0	32.0	32.0	32.0	32.0
3	31.362772818186254	32.0	32.0	32.0	32.0	32.0
4	31.467019854627054	32.0	32.0	32.0	32.0	32.0
5	31.390315625152446	32.0	32.0	32.0	32.0	32.0
6	34.91209717547198	36.0	36.0	36.0	36.0	36.0
7	34.92468120396117	36.0	36.0	36.0	36.0	36.0
8	34.88284306551539	36.0	36.0	36.0	32.0	36.0
9	34.98273867017903	36.0	36.0	36.0	36.0	36.0
10	34.823136738377485	36.0	36.0	36.0	32.0	36.0
11	35.00110639543392	36.0	36.0	36.0	36.0	36.0
12	34.89522025464657	36.0	36.0	36.0	32.0	36.0
13	34.9470803453827	36.0	36.0	36.0	36.0	36.0
14	34.900966876432996	36.0	36.0	36.0	32.0	36.0
15	34.86388018927752	36.0	36.0	36.0	32.0	36.0
16	34.87142592321577	36.0	36.0	36.0	32.0	36.0
17	34.83381238109176	36.0	36.0	36.0	32.0	36.0
18	34.84063612859164	36.0	36.0	36.0	32.0	36.0
19	34.8268598468218	36.0	36.0	36.0	32.0	36.0
20	34.80199229230694	36.0	36.0	36.0	32.0	36.0
21	34.78292599639007	36.0	36.0	36.0	32.0	36.0
22	34.77356553978243	36.0	36.0	36.0	32.0	36.0
23	34.708395531489344	36.0	36.0	36.0	32.0	36.0
24	34.68629298990194	36.0	36.0	36.0	32.0	36.0
25	34.66603444070442	36.0	36.0	36.0	32.0	36.0
26	34.59457144250939	36.0	36.0	36.0	32.0	36.0
27	34.58713888482365	36.0	36.0	36.0	32.0	36.0
28	34.5465125128055	36.0	36.0	36.0	32.0	36.0
29	34.496535440753206	36.0	36.0	36.0	32.0	36.0
30	34.47356456412508	36.0	36.0	36.0	32.0	36.0
31	34.465478316015414	36.0	36.0	36.0	32.0	36.0
32	34.42713107956486	36.0	36.0	36.0	32.0	36.0
33	34.387794526562274	36.0	36.0	36.0	32.0	36.0
34	34.36161763988487	36.0	36.0	36.0	32.0	36.0
35	34.3128581882043	36.0	36.0	36.0	32.0	36.0
36	34.26595638811649	36.0	36.0	36.0	32.0	36.0
37	34.25757354017269	36.0	36.0	36.0	32.0	36.0
38	33.81525732962584	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	12.0
22	41.0
23	109.0
24	337.0
25	838.0
26	1806.0
27	3599.0
28	6317.0
29	10088.0
30	15337.0
31	21949.0
32	30787.0
33	48785.0
34	116475.0
35	255993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52791259057175	18.994455925485767	12.433284742183947	25.044346741758535
2	15.03117225230499	22.045368066734962	37.38133567491097	25.54212400604908
3	17.83208937021318	26.2555246597395	29.210985901751307	26.701400068296017
4	11.554319722913313	17.238694570466855	36.990097077906235	34.21688862871359
5	13.262500609785842	38.374359724864625	33.47538904336797	14.88775062198156
6	32.303624567052054	37.1583004048978	17.42836235913947	13.109712668910678
7	28.40163598543536	31.698973996729592	21.721492212287842	18.177897805547207
8	27.66150922115353	33.22405715144313	20.156382021726447	18.9580516056769
9	27.424563813657056	14.11973390730467	19.010914171641108	39.444788107397166
10	15.634311538844301	27.444977159594043	32.02320530607705	24.897505995484607
11	36.93909052413021	21.830074797687185	22.588784444891104	18.642050233291506
12	23.60202391150363	25.097907597083164	29.8105461165759	21.489522374837307
13	29.372083992717663	20.28711756521807	25.832190183677973	24.508608258386293
14	23.679789258012583	20.352992828918484	25.9747304746573	29.99248743841163
15	24.67925264647056	27.569930240499534	23.844675350017074	23.90614176301283
16	24.55085613932387	26.464120200985413	25.264061661544467	23.72096199814625
17	22.9203375774428	26.252792819161915	26.503731889360456	24.323137714034832
18	24.096394946094932	24.996731547880387	27.977950143909457	22.928923362115224
19	24.247036440801992	26.038733596760817	26.87526220791258	22.83896775452461
20	24.70910776135421	24.461095663203082	27.031367383774818	23.798429191667886
21	26.300261086416093	24.73881601798335	25.610665126425925	23.350257769174632
22	25.059368749695103	25.079272159617545	26.4822674276794	23.37909166300795
23	23.34659582065787	24.98063312603786	26.891172803250125	24.78159825005415
24	23.368359432167424	26.107419874140202	26.639348260890777	23.8848724328016
25	23.94672910873701	25.034977315966632	26.78862383530904	24.229669739987315
26	23.205034391921558	26.089467778916042	27.524854870969317	23.180642958193083
27	24.78955029913713	25.19522941651674	26.650132494526552	23.36508778981958
28	23.40758085760281	25.406702765988587	27.567393531391776	23.61832284501683
29	23.07975998829211	25.692375237816478	27.459680960046835	23.76818381384458
30	23.35157812576223	25.760866383726032	27.77169618030148	23.115859310210254
31	23.41987414020196	25.85062685984682	26.348016976437876	24.381482023513342
32	23.499292648421875	25.84106541782526	26.483633347968194	24.176008585784672
33	23.02746475437826	25.51246402263525	27.052441582516217	24.407629640470265
34	23.934240694668034	25.93785062685985	27.115469047270597	23.012439631201524
35	24.93253329430704	25.21488853114786	27.04541684960242	22.80716132494268
36	23.160544416800818	26.24830479535587	26.751939118981415	23.839211668861896
37	24.404312405483193	26.40148299917069	26.403044050929314	22.7911605444168
38	23.292063027464753	25.71618127713547	26.802673301136643	24.189082394263135
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	384.0
1	276.5
2	169.0
3	169.0
4	499.5
5	830.0
6	830.0
7	1012.5
8	1195.0
9	1115.5
10	1036.0
11	1036.0
12	1301.0
13	1566.0
14	1921.5
15	2277.0
16	2277.0
17	3346.5
18	4416.0
19	4416.0
20	5034.5
21	5653.0
22	5856.0
23	6059.0
24	6059.0
25	6748.0
26	7437.0
27	7437.0
28	9538.5
29	11640.0
30	13621.0
31	15602.0
32	15602.0
33	18229.0
34	20856.0
35	20856.0
36	22671.5
37	24487.0
38	27679.5
39	30872.0
40	30872.0
41	32186.5
42	33501.0
43	37350.5
44	41200.0
45	41200.0
46	43621.0
47	46042.0
48	46042.0
49	46475.0
50	46908.0
51	45630.0
52	44352.0
53	44352.0
54	41045.5
55	37739.0
56	37739.0
57	36266.0
58	34793.0
59	30732.5
60	26672.0
61	26672.0
62	25495.0
63	24318.0
64	20171.5
65	16025.0
66	16025.0
67	13651.5
68	11278.0
69	11278.0
70	9084.0
71	6890.0
72	5177.5
73	3465.0
74	3465.0
75	2655.0
76	1845.0
77	1845.0
78	1598.5
79	1352.0
80	1040.5
81	729.0
82	729.0
83	658.0
84	587.0
85	587.0
86	378.0
87	169.0
88	131.5
89	94.0
90	94.0
91	59.5
92	25.0
93	15.5
94	6.0
95	6.0
96	4.5
97	3.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007024732913800673
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.9513146982779649E-4
8	0.002341577637933558
9	0.004292892336211523
10	0.001170788818966779
11	0.005073418215522709
12	3.9026293965559297E-4
13	3.9026293965559297E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.9513146982779649E-4
22	0.0
23	3.9026293965559297E-4
24	0.0
25	0.0
26	0.0
27	1.9513146982779649E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	512475.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.415077642763684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.22283335050308	54.678276715012274
2	9.79780886858385	12.42657620260407
3	1.8972836709607601	3.6094917391297296
4	0.6228872774549943	1.580017802499926
5	0.28998864652237955	0.9194826267368326
6	0.17166249816625953	0.6531594389738477
7	0.11175985529092794	0.496108193043276
8	0.08464579437759419	0.4294255698070837
9	0.06793459964480893	0.38772701199950627
>10	0.5610257568973148	7.430075749367368
>50	0.08809400281063272	3.92759509717425
>100	0.07758454182226836	9.651883050668575
>500	0.004945628163969294	2.1618728303376056
>1k	0.0015455088012404047	1.6483079726456547
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2746	0.5358310161471291	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2198	0.4288989706814967	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1247	0.2433289428752622	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1123	0.21913264061661544	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1095	0.21366895946143713	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	966	0.1884969998536514	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	915	0.1785452948924338	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	843	0.16449582906483243	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	763	0.14888531147860873	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	751	0.14654373384067515	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	725	0.14147031562515244	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	725	0.14147031562515244	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	704	0.13737255475876872	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	694	0.13542124006049075	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	664	0.12956729596565686	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	591	0.11532269866822771	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	564	0.11005414898287721	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	557	0.10868822869408264	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	554	0.10810283428459924	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	1.9513146982779649E-4	0.0
16	0.0	0.0	0.0	1.9513146982779649E-4	0.0
17	0.0	0.0	0.0	1.9513146982779649E-4	0.0
18	0.0	0.0	0.0	3.9026293965559297E-4	0.0
19	0.0	0.0	0.0	3.9026293965559297E-4	0.0
20	0.0	0.0	0.0	5.853944094833894E-4	0.0
21	0.0	0.0	0.0	0.0013659202887945753	0.0
22	0.0	0.0	0.0	0.0021464461681057613	0.0
23	0.0	0.0	0.0	0.002926972047416947	0.0
24	0.0	0.0	0.0	0.005853944094833894	0.0
25	0.0	0.0	0.0	0.007610127323284062	0.0
26	0.0	0.0	0.0	0.010341967900873213	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	175	0.0	23.77143	29
TCTAGCG	170	0.0	23.529411	28
TAGACTG	55	2.214545E-6	23.272728	5
CGCGGTC	75	6.3850166E-8	21.333332	10
GAACCGC	85	1.053013E-8	20.705883	6
TAGCGGC	195	0.0	20.512821	30
GGATAGG	40	0.004480149	20.0	1
GCCGCGC	40	0.004480149	20.0	8
AGTGCGG	40	0.004480149	20.0	10
CAGTGCG	40	0.004480149	20.0	9
AGCGGCG	210	0.0	19.809525	31
GCATAAA	50	7.1884954E-4	19.2	1
CGAATGT	50	7.1884954E-4	19.2	23
AACCGCG	85	2.3895518E-7	18.82353	7
TCGCGTA	105	5.9535523E-9	18.285715	9
CGGTCCA	220	0.0	18.181818	10
CGCGTAA	115	9.367795E-10	18.086956	10
TACCGTC	205	0.0	17.95122	7
AGACGTT	45	0.008843771	17.777779	5
ACAATAC	45	0.008843771	17.777779	3
>>END_MODULE