FastQCFastQC Report
Thu 2 Feb 2017
SRR4062885_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062885_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350312
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42130.31200196695282273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26270.1945476304735498No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18700.13848651274668372No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18570.13752377228373888No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15820.11715810864452068No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15550.11515857075994287No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15400.1140477163796219No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15350.11367743158618156No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14980.11093732411472312No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14960.11078921019734699No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14900.11034486844521858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14640.10841938751932888No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT13960.10338351432854036No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13900.10293917257641197No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13760.10190237515477904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG1055.991751E-918.2826674
TCGTTTA2900.018.20723330
CTAGCGG2850.017.40383129
GGACGTG1201.6625563E-917.3310876
CATCGTT3150.017.2701628
ATCGTTT3250.016.73877129
TCTAGCG3050.016.26259428
GGCATCG3250.016.2452526
CGTTTAT3300.016.00029631
GTTATTC2600.015.9997033
AACCGAC805.6246987E-515.99911117
ATGCGCA600.002443107515.99911111
GCGAGGG1304.80577E-915.9991118
TACACCG805.6300458E-515.9973345
CGCAAGA3650.015.7863752
GCATCGT3250.015.7529727
AAACGCT3900.015.59003229
CGCTTCG3900.015.59003232
TAACCCG1753.6379788E-1215.54314528
ATGGGCG1755.456968E-1215.5402675
Produced by FastQC (version 0.11.5)