FastQCFastQC Report
Thu 2 Feb 2017
SRR4062885_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062885_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350312
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT44730.33125677621171995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31560.23372376161953679No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19360.1433742720200961No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18960.14041199367257345No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18840.13952331016831665No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17150.12700768415003347No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16480.12204586791793304No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16420.12160152616580464No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15630.11575102642944742No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15340.1136033746274935No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14990.11101138107341119No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT14020.10382785608066877No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG13980.10353162824591651No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13970.10345757128722842No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13890.1028651156177239No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG13610.10079152077445805No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13570.1004952929397058No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13510.10005095118757738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTT400.00448471919.99935229
GTTCGCG601.1383291E-418.66675211
TCTAGCG3750.017.49276528
TTCGCGT652.101266E-417.23021112
ACGCTTA753.2352662E-517.06611417
AAGACGG3850.016.6253015
CTAGCGG3950.016.60705829
TAGGACG1750.016.4584394
GATATAC3600.016.443911
TAGCGGC3850.016.20726830
CAAGACG3950.016.2038084
TACCGTC3500.016.0018527
ACCGTCC703.69374E-416.001858
CGTAAAT908.579635E-616.0006663
CATCGTT2700.015.99948228
CGTCGTA3650.015.7814810
ATCGTTT2750.015.70858229
CCGATAA1753.6379788E-1215.5435059
CGATAAC1753.6379788E-1215.54350510
GATAACG1753.6379788E-1215.5429311
Produced by FastQC (version 0.11.5)