##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062885_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1350312
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.181246260123586	32.0	32.0	32.0	32.0	32.0
2	31.29215248031566	32.0	32.0	32.0	32.0	32.0
3	31.388020694476534	32.0	32.0	32.0	32.0	32.0
4	31.48130876419672	32.0	32.0	32.0	32.0	32.0
5	31.408804039362753	32.0	32.0	32.0	32.0	32.0
6	34.947337356107326	36.0	36.0	36.0	36.0	36.0
7	34.97406303135868	36.0	36.0	36.0	36.0	36.0
8	34.90666453382626	36.0	36.0	36.0	36.0	36.0
9	35.02066929716984	36.0	36.0	36.0	36.0	36.0
10	34.86667155442594	36.0	36.0	36.0	32.0	36.0
11	35.052698931802425	36.0	36.0	36.0	36.0	36.0
12	34.941604606935286	36.0	36.0	36.0	36.0	36.0
13	34.99824410950951	36.0	36.0	36.0	36.0	36.0
14	34.94033082724585	36.0	36.0	36.0	32.0	36.0
15	34.906942247421334	36.0	36.0	36.0	32.0	36.0
16	34.92072128515484	36.0	36.0	36.0	32.0	36.0
17	34.88552423439916	36.0	36.0	36.0	32.0	36.0
18	34.88984990135613	36.0	36.0	36.0	32.0	36.0
19	34.88163254122011	36.0	36.0	36.0	32.0	36.0
20	34.861611242438784	36.0	36.0	36.0	32.0	36.0
21	34.86087881911736	36.0	36.0	36.0	32.0	36.0
22	34.839243819206224	36.0	36.0	36.0	32.0	36.0
23	34.77933025848841	36.0	36.0	36.0	32.0	36.0
24	34.75172478656785	36.0	36.0	36.0	32.0	36.0
25	34.73964683717541	36.0	36.0	36.0	32.0	36.0
26	34.67457817156331	36.0	36.0	36.0	32.0	36.0
27	34.66349999111316	36.0	36.0	36.0	32.0	36.0
28	34.62857546996546	36.0	36.0	36.0	32.0	36.0
29	34.58457600910012	36.0	36.0	36.0	32.0	36.0
30	34.55752522380013	36.0	36.0	36.0	32.0	36.0
31	34.5580347356759	36.0	36.0	36.0	32.0	36.0
32	34.53460977907328	36.0	36.0	36.0	32.0	36.0
33	34.48650534098786	36.0	36.0	36.0	32.0	36.0
34	34.47807025339329	36.0	36.0	36.0	32.0	36.0
35	34.43467213503249	36.0	36.0	36.0	32.0	36.0
36	34.407779091054515	36.0	36.0	36.0	32.0	36.0
37	34.39440366374586	36.0	36.0	36.0	32.0	36.0
38	33.956144209634516	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	24.0
22	72.0
23	271.0
24	772.0
25	2029.0
26	4359.0
27	8646.0
28	15244.0
29	24175.0
30	36972.0
31	53528.0
32	77954.0
33	124496.0
34	305401.0
35	696363.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99906976193015	18.595873772026497	12.211448445850664	26.193608020192684
2	15.4954558650149	20.99048219966941	37.09090936020713	26.423152575108567
3	18.129291600755973	24.741393100261273	28.96500956815906	28.164305730823692
4	11.869182825895052	16.17937187849919	36.7186990858409	35.232746209764855
5	13.807475605637809	37.3461096398462	33.564539158357476	15.281875596158518
6	33.55676317769523	36.53451942958368	16.707916392655918	13.200801000065171
7	29.454809638457853	31.089484625257075	21.0029778589779	18.452727877307172
8	27.865598743968867	33.2217272649848	19.68125247632907	19.231421514717265
9	27.296722633624164	14.089498252560059	18.68009221920799	39.93368689460778
10	15.445718479482013	27.092363713921984	31.820783927755475	25.641133878840527
11	37.58505870050154	21.381172588065564	22.12703777983831	18.906730931594588
12	24.223418491619324	24.153730738667964	29.082010117684177	22.540840652028535
13	29.297747483544544	19.545556878706552	25.67051170396175	25.486183933787153
14	23.254551540680968	19.985455213313664	25.502698635574596	31.257294610430776
15	24.887211251918075	27.45476600963333	22.823910325909864	24.83411241253873
16	25.369470166894764	26.166026814543603	24.194186232515154	24.270316786046482
17	23.68600738199764	26.096709501211574	25.633705395493784	24.583577721297004
18	24.57083992440266	25.140115765837823	26.801435520087207	23.48760878967231
19	25.1253784310589	25.372950843953102	25.93748704003223	23.564183684955772
20	25.275862171113044	24.531885964132734	25.785077819052187	24.40717404570203
21	26.543830675918862	24.58354007598255	24.918944538661492	23.95368470943709
22	25.063559431864213	24.763184184976648	25.793243186199327	24.380013196959812
23	23.886070606008992	24.26198428509009	26.212203123727146	25.639741985173774
24	24.06488278264579	25.508030736600134	25.872687201180174	24.554399279573904
25	24.48063854872059	24.81767176770998	25.881129694470612	24.820559989098815
26	23.926265875046564	25.847378863091542	26.30223704020778	23.924118221654123
27	24.9240544763795	25.046396753338122	25.614414467788876	24.415134302493502
28	23.874260170982705	25.174922536421214	26.56178720177263	24.389030090823454
29	23.838564716895057	25.193436776093233	26.349465901213943	24.61853260579777
30	23.97179318557489	25.64244411661897	26.406045417651626	23.97971728015451
31	24.53558881206714	25.369544223853453	25.3239251373016	24.77094182677781
32	24.235732186339156	25.402277399593576	25.472927738181987	24.889062675885278
33	23.963054464449698	24.703475937412982	26.09174768497947	25.24172191315785
34	24.490414067267416	25.325998732144868	26.222236046187845	23.96135115439987
35	25.3499931867598	24.947419559331475	26.036501193798173	23.66608606011055
36	23.90706740368152	25.885128770239767	25.707984525057913	24.4998193010208
37	25.071779760366315	25.58647600441676	25.42740153934908	23.91434269586784
38	24.169895550065466	25.156408296749195	25.986808974518482	24.686887178666854
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	81.5
2	107.0
3	107.0
4	415.0
5	723.0
6	723.0
7	1015.5
8	1308.0
9	1420.0
10	1532.0
11	1532.0
12	1962.5
13	2393.0
14	3086.5
15	3780.0
16	3780.0
17	5734.0
18	7688.0
19	7688.0
20	9175.5
21	10663.0
22	11763.5
23	12864.0
24	12864.0
25	15640.5
26	18417.0
27	18417.0
28	23100.0
29	27783.0
30	32974.0
31	38165.0
32	38165.0
33	45603.5
34	53042.0
35	53042.0
36	57997.0
37	62952.0
38	71293.5
39	79635.0
40	79635.0
41	83485.5
42	87336.0
43	97076.0
44	106816.0
45	106816.0
46	109448.0
47	112080.0
48	112080.0
49	116331.0
50	120582.0
51	119741.0
52	118900.0
53	118900.0
54	112279.5
55	105659.0
56	105659.0
57	102397.0
58	99135.0
59	88254.5
60	77374.0
61	77374.0
62	74256.5
63	71139.0
64	59243.5
65	47348.0
66	47348.0
67	39929.5
68	32511.0
69	32511.0
70	26399.5
71	20288.0
72	15925.0
73	11562.0
74	11562.0
75	8842.0
76	6122.0
77	6122.0
78	5713.5
79	5305.0
80	4177.0
81	3049.0
82	3049.0
83	2851.0
84	2653.0
85	2653.0
86	1685.5
87	718.0
88	581.5
89	445.0
90	445.0
91	282.0
92	119.0
93	80.0
94	41.0
95	41.0
96	26.5
97	12.0
98	12.0
99	11.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00888683504256794
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	9.627404629448602E-4
8	0.0019995378845777865
9	0.003776904893091375
10	6.665126281925956E-4
11	0.005035873190788499
12	2.2217087606419848E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.4811391737613234E-4
22	7.405695868806617E-5
23	1.4811391737613234E-4
24	0.0
25	0.0
26	7.405695868806617E-5
27	1.4811391737613234E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	7.405695868806617E-5
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1350312.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.82459039602217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.29153636926863	41.35718340181888
2	13.530490021399647	14.294851864758032
3	3.865292313054538	6.125474495939971
4	1.521501341982233	3.21490740708838
5	0.7298052547627952	1.9275831825854632
6	0.423475982037771	1.3421967176219094
7	0.28141274931651344	1.0405859250404303
8	0.20173884005680698	0.8525417274375566
9	0.1438617250582597	0.6839493029880953
>10	0.8161075003748208	8.019057594393013
>50	0.08740454015507484	3.233085494630225
>100	0.09098481687083511	10.055697765107617
>500	0.011726286982363656	4.1261921114889795
>1k	0.004662258679734948	3.7266930091014725
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4473	0.33125677621171995	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3156	0.23372376161953679	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1936	0.1433742720200961	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1896	0.14041199367257345	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1884	0.13952331016831665	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1715	0.12700768415003347	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1648	0.12204586791793304	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1642	0.12160152616580464	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1563	0.11575102642944742	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1534	0.1136033746274935	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1499	0.11101138107341119	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1402	0.10382785608066877	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1398	0.10353162824591651	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1397	0.10345757128722842	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1389	0.1028651156177239	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1361	0.10079152077445805	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1357	0.1004952929397058	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1351	0.10005095118757738	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	7.405695868806617E-5
7	0.0	0.0	0.0	0.0	7.405695868806617E-5
8	0.0	0.0	0.0	0.0	7.405695868806617E-5
9	0.0	0.0	0.0	0.0	7.405695868806617E-5
10	0.0	0.0	0.0	0.0	7.405695868806617E-5
11	0.0	0.0	0.0	0.0	7.405695868806617E-5
12	0.0	0.0	0.0	7.405695868806617E-5	2.221708760641985E-4
13	0.0	0.0	0.0	7.405695868806617E-5	2.221708760641985E-4
14	0.0	0.0	0.0	7.405695868806617E-5	2.221708760641985E-4
15	0.0	0.0	0.0	2.221708760641985E-4	3.702847934403308E-4
16	0.0	0.0	0.0	2.962278347522647E-4	3.702847934403308E-4
17	0.0	0.0	0.0	2.962278347522647E-4	3.702847934403308E-4
18	0.0	0.0	0.0	3.702847934403308E-4	4.44341752128397E-4
19	0.0	0.0	0.0	3.702847934403308E-4	5.183987108164632E-4
20	0.0	0.0	0.0	3.702847934403308E-4	5.183987108164632E-4
21	0.0	0.0	0.0	4.44341752128397E-4	5.183987108164632E-4
22	0.0	0.0	0.0	8.88683504256794E-4	5.183987108164632E-4
23	0.0	0.0	0.0	0.001851423967201654	5.183987108164632E-4
24	0.0	0.0	0.0	0.0029622783475226466	5.183987108164632E-4
25	0.0	0.0	0.0	0.0034806770583391097	5.183987108164632E-4
26	0.0	0.0	0.0	0.00444341752128397	5.183987108164632E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTT	40	0.004484719	19.999352	29
GTTCGCG	60	1.1383291E-4	18.666752	11
TCTAGCG	375	0.0	17.492765	28
TTCGCGT	65	2.101266E-4	17.230211	12
ACGCTTA	75	3.2352662E-5	17.066114	17
AAGACGG	385	0.0	16.625301	5
CTAGCGG	395	0.0	16.607058	29
TAGGACG	175	0.0	16.458439	4
GATATAC	360	0.0	16.44391	1
TAGCGGC	385	0.0	16.207268	30
CAAGACG	395	0.0	16.203808	4
TACCGTC	350	0.0	16.001852	7
ACCGTCC	70	3.69374E-4	16.00185	8
CGTAAAT	90	8.579635E-6	16.000666	3
CATCGTT	270	0.0	15.999482	28
CGTCGTA	365	0.0	15.78148	10
ATCGTTT	275	0.0	15.708582	29
CCGATAA	175	3.6379788E-12	15.543505	9
CGATAAC	175	3.6379788E-12	15.543505	10
GATAACG	175	3.6379788E-12	15.54293	11
>>END_MODULE