FastQCFastQC Report
Thu 2 Feb 2017
SRR4062884_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062884_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166861
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA211340.97532790520481No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA182090.8403400125804101No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC171570.7917905209425061No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG152130.7020754907675204No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA87600.40427143226999795No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA86690.4000718089439055No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA80100.36965915210989536No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA66780.3081877425455532No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA59500.27459075593681365No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG59100.27274476766160816No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT58380.26942198876623835No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA51480.237578691018944No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT51140.23600960098501936No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT46300.21367314285503317No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA44820.20684298623677289No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT44070.20338175822076265No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG40500.18690631286455384No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA39720.18330663572790318No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT39680.18312203690038265No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT38590.17809171885044772No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG38510.17772252119540663No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT36200.16706193890609505No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA34060.15718590163374577No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA32860.15164793680812935No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC32610.15049419413612594No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA32450.14975579882604376No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA31410.14495622931050953No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31180.14389478605226638No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT30190.13932596507113285No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA28650.13221891021159177No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT26630.12289666942180417No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA25730.11874319580259186No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT25470.1175433034237083No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT25440.11740485430306791No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT24810.11449742276961929No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG24570.113389829804496No Hit
CTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGT23750.10960555384032479No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA23070.10646737377247549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC52350.022.5823334
CACGATT1350.022.51650428
GTCCTAG5950.020.7308241
CTATAGG7550.020.5810531
TATAGGA8150.020.2302552
TTCATCG801.2679811E-719.99821328
CGGTAGG1550.019.6365281
TCTAGCG1550.019.61114928
AGGACCT87400.019.6014865
TCCTATA8500.019.5855672
GTCCTAT9400.019.427631
CGATTTT1900.019.3680330
GGACCTG85200.019.281996
TCACGAT1750.019.19739727
AAGACGG2000.019.1951835
TAGCGGC1600.018.99961730
CATCGTT1351.8189894E-1218.96126728
TGTAGGA157900.018.9372332
CTCTAGG7050.018.6323451
CTGTAGG155250.018.5421141
Produced by FastQC (version 0.11.5)