##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062884_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2166861
Sequences flagged as poor quality	0
Sequence length	38
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.376680368514638	32.0	32.0	32.0	32.0	32.0
2	31.17805572207908	32.0	32.0	32.0	32.0	32.0
3	31.191936630914487	32.0	32.0	32.0	32.0	32.0
4	31.232496223800233	32.0	32.0	32.0	32.0	32.0
5	31.242120283673017	32.0	32.0	32.0	32.0	32.0
6	34.92226820271351	36.0	36.0	36.0	36.0	36.0
7	34.8484152882903	36.0	36.0	36.0	36.0	36.0
8	34.83839849441196	36.0	36.0	36.0	32.0	36.0
9	34.91547635035196	36.0	36.0	36.0	36.0	36.0
10	34.73030895844265	36.0	36.0	36.0	32.0	36.0
11	34.87732115719467	36.0	36.0	36.0	36.0	36.0
12	34.76010505519274	36.0	36.0	36.0	32.0	36.0
13	34.79844253969221	36.0	36.0	36.0	32.0	36.0
14	34.77326510560668	36.0	36.0	36.0	32.0	36.0
15	34.73055908985394	36.0	36.0	36.0	32.0	36.0
16	34.79352021195638	36.0	36.0	36.0	32.0	36.0
17	34.73831593258635	36.0	36.0	36.0	32.0	36.0
18	34.79815733450369	36.0	36.0	36.0	32.0	36.0
19	34.676985741125065	36.0	36.0	36.0	32.0	36.0
20	34.62051972876894	36.0	36.0	36.0	32.0	36.0
21	34.56807427887622	36.0	36.0	36.0	32.0	36.0
22	34.527970183597375	36.0	36.0	36.0	32.0	36.0
23	34.552257851334254	36.0	36.0	36.0	32.0	36.0
24	34.489651158980664	36.0	36.0	36.0	32.0	36.0
25	34.46031102133455	36.0	36.0	36.0	32.0	36.0
26	34.504030946147445	36.0	36.0	36.0	32.0	36.0
27	34.4259848693571	36.0	36.0	36.0	32.0	36.0
28	34.30582810803277	36.0	36.0	36.0	32.0	36.0
29	34.21126228216762	36.0	36.0	36.0	32.0	36.0
30	34.1338059986312	36.0	36.0	36.0	32.0	36.0
31	34.11598713530771	36.0	36.0	36.0	32.0	36.0
32	34.0843704326212	36.0	36.0	36.0	32.0	36.0
33	34.16017640263958	36.0	36.0	36.0	32.0	36.0
34	34.10873009390081	36.0	36.0	36.0	32.0	36.0
35	34.13516787648123	36.0	36.0	36.0	32.0	36.0
36	34.01467376079961	36.0	36.0	36.0	32.0	36.0
37	33.896621426109014	36.0	36.0	36.0	32.0	36.0
38	33.23598560313744	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	0.0
6	0.0
7	5.0
8	3.0
9	4.0
10	23.0
11	30.0
12	12.0
13	12.0
14	136.0
15	359.0
16	517.0
17	657.0
18	938.0
19	1144.0
20	1469.0
21	1883.0
22	2626.0
23	3799.0
24	5844.0
25	9041.0
26	13959.0
27	21292.0
28	31885.0
29	44979.0
30	64637.0
31	89499.0
32	123986.0
33	194094.0
34	489089.0
35	1064938.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.00519664874841	19.31812353200218	12.168226138782776	26.508453680466637
2	10.060278461186101	18.991178491477715	52.95943509837385	17.989107948962328
3	20.892208819622464	24.674440523258337	29.255027974990977	25.178322682128222
4	9.995371060497886	16.58595636059208	40.674209884331894	32.74446269457814
5	9.584947038474176	41.58559682784643	37.66415488615764	11.165301247521755
6	30.3848665462156	39.918637841479935	18.446873464075814	11.249622148228653
7	27.039713207261563	30.62563773126195	21.974736727459675	20.359912334016812
8	20.332491559183552	48.854098018692554	18.827266467668306	11.986143954455589
9	27.48462633632615	13.617524015220214	20.34843125174504	38.549418396708596
10	15.921020690902814	28.827429360462165	36.21120282659682	19.040347122038206
11	35.36836042528722	17.856737390957615	31.068968413590802	15.70593377016436
12	28.933933545875277	20.995085813655972	35.914542631766466	14.156438008702283
13	34.077740662290665	23.21095992589006	24.960362536816106	17.750936875003173
14	16.990145182253205	28.1030768151474	25.99586644118863	28.910911561410764
15	18.212334910352705	45.33709885367971	20.450182175049612	16.000384060917973
16	15.91670651397302	26.25154389080792	40.531495372481544	17.300254222737518
17	16.77510303955731	33.06253089801935	33.19062352722326	16.971742535200086
18	17.00633491513909	25.55468583417716	43.178820356280134	14.260158894403622
19	25.949941085168277	23.0227070176726	26.396936890284568	24.63041500687455
20	25.245625176534443	29.177981793645312	30.594180317125087	14.982212712695157
21	20.130816569729607	26.229497604188495	25.536046094102637	28.103639731979264
22	23.23292089295365	35.85277498422714	26.245847942744188	14.668456180075019
23	17.193074603278635	34.448642456455495	31.484828540491844	16.873454399774026
24	25.57671741296793	26.0385621424869	33.24968186440154	15.13503858014363
25	19.954414386885965	31.308767752932994	32.95092992158222	15.785887938598819
26	13.619272085405889	34.53033777570984	35.2814889900702	16.568901148814074
27	16.33343302649992	32.8633908230594	32.529418714992005	18.273757435448676
28	15.20150678950638	34.50140903833711	27.334420688171924	22.962663483984592
29	19.567347567997437	28.345477614812964	26.4100390060136	25.677135811176
30	15.539194352538955	30.205341661516933	31.636821290957684	22.61864269498643
31	24.611876993229366	25.987695630702774	29.323721217883335	20.07670615818453
32	18.2467430870647	33.16676658825245	30.172488195176168	18.41400212950668
33	15.66208897083767	33.41028723868249	35.48287344836419	15.444750342115649
34	18.182330096549638	32.62697352570026	34.27258217427306	14.918114203477042
35	15.968435347020089	34.33214522768333	33.64383786742883	16.05558155786776
36	14.280063382430253	31.771066280611727	36.00590067716548	17.942969659792542
37	16.051807837269262	31.413324520843965	27.953699353186668	24.581168288700106
38	18.514472436256895	36.830948375528735	26.97598427104751	17.67859491716685
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	73.0
1	120.0
2	167.0
3	167.0
4	397.0
5	627.0
6	627.0
7	857.5
8	1088.0
9	1265.5
10	1443.0
11	1443.0
12	1819.0
13	2195.0
14	2989.0
15	3783.0
16	3783.0
17	5763.5
18	7744.0
19	7744.0
20	9975.0
21	12206.0
22	16696.5
23	21187.0
24	21187.0
25	39757.0
26	58327.0
27	58327.0
28	84857.5
29	111388.0
30	161242.0
31	211096.0
32	211096.0
33	263954.0
34	316812.0
35	316812.0
36	327695.5
37	338579.0
38	333401.5
39	328224.0
40	328224.0
41	272239.5
42	216255.0
43	169078.0
44	121901.0
45	121901.0
46	102318.0
47	82735.0
48	82735.0
49	76039.0
50	69343.0
51	64905.0
52	60467.0
53	60467.0
54	54665.5
55	48864.0
56	48864.0
57	45273.5
58	41683.0
59	35750.0
60	29817.0
61	29817.0
62	28668.5
63	27520.0
64	22764.5
65	18009.0
66	18009.0
67	15446.5
68	12884.0
69	12884.0
70	10654.5
71	8425.0
72	6490.5
73	4556.0
74	4556.0
75	3542.5
76	2529.0
77	2529.0
78	2325.0
79	2121.0
80	1799.5
81	1478.0
82	1478.0
83	1388.0
84	1298.0
85	1298.0
86	933.0
87	568.0
88	433.5
89	299.0
90	299.0
91	232.5
92	166.0
93	151.5
94	137.0
95	137.0
96	124.5
97	112.0
98	112.0
99	433.5
100	755.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12801928688549935
2	0.057456385065770256
3	0.013060367047078702
4	0.0026766829990479317
5	2.3074853440068375E-4
6	2.768982412808205E-4
7	0.0
8	4.153473619212307E-4
9	2.768982412808205E-4
10	6.922456032020512E-4
11	0.0019844373958458803
12	0.0033689286022499827
13	0.016152397408047865
14	0.009460689910428033
15	0.024828542301513573
16	0.011860474668195144
17	0.021228865164862904
18	0.006414809256339008
19	0.008030048997143794
20	0.006691707497619829
21	0.00752240222146229
22	0.007568551928342428
23	0.010475983461791042
24	0.0153217026842054
25	0.017906086269493058
26	0.020905817216701948
27	0.009829887565469129
28	0.0062302104288184616
29	0.012552720271397196
30	0.003784275964171214
31	0.006968605738900649
32	0.007476252514582154
33	0.009045342548506804
34	0.014167960012201984
35	0.01785993656261292
36	0.015229403270445127
37	0.011029779944352683
38	0.007199354273301333
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2166861.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.22478813603175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0394506852064	28.301423161501855
2	13.921433391654059	10.642876838917086
3	4.872933115385174	5.588005078099544
4	2.1742310791044837	3.324380894301783
5	1.20134729636914	2.296062294075246
6	0.7636778394102369	1.7514854169383267
7	0.48770848427597296	1.3049787438515967
8	0.3614166862548919	1.105206100873592
9	0.25662086261890743	0.88283502944041
>10	1.5886315481123796	11.634302219729411
>50	0.16471696051161944	4.383622374298205
>100	0.14058750166560538	10.889433884648692
>500	0.015378034567911295	4.123919263891769
>1k	0.010292385340728032	7.671541430713834
>5k	0.0010897819772535565	2.780676307176657
>10k+	4.8434754544602503E-4	3.3192509615420147
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	21134	0.97532790520481	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	18209	0.8403400125804101	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	17157	0.7917905209425061	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	15213	0.7020754907675204	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	8760	0.40427143226999795	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	8669	0.4000718089439055	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	8010	0.36965915210989536	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	6678	0.3081877425455532	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	5950	0.27459075593681365	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	5910	0.27274476766160816	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	5838	0.26942198876623835	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	5148	0.237578691018944	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	5114	0.23600960098501936	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4630	0.21367314285503317	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	4482	0.20684298623677289	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	4407	0.20338175822076265	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	4050	0.18690631286455384	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	3972	0.18330663572790318	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	3968	0.18312203690038265	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT	3859	0.17809171885044772	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	3851	0.17772252119540663	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT	3620	0.16706193890609505	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA	3406	0.15718590163374577	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA	3286	0.15164793680812935	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC	3261	0.15049419413612594	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA	3245	0.14975579882604376	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	3141	0.14495622931050953	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3118	0.14389478605226638	No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT	3019	0.13932596507113285	No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA	2865	0.13221891021159177	No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT	2663	0.12289666942180417	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA	2573	0.11874319580259186	No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT	2547	0.1175433034237083	No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT	2544	0.11740485430306791	No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT	2481	0.11449742276961929	No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG	2457	0.113389829804496	No Hit
CTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGT	2375	0.10960555384032479	No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA	2307	0.10646737377247549	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	4.614970688013675E-5	0.0
9	0.0	0.0	0.0	4.614970688013675E-5	0.0
10	0.0	0.0	4.614970688013675E-5	4.614970688013675E-5	0.0
11	0.0	0.0	4.614970688013675E-5	4.614970688013675E-5	0.0
12	0.0	0.0	4.614970688013675E-5	9.22994137602735E-5	0.0
13	0.0	0.0	4.614970688013675E-5	9.22994137602735E-5	0.0
14	0.0	0.0	4.614970688013675E-5	9.22994137602735E-5	0.0
15	0.0	0.0	4.614970688013675E-5	9.22994137602735E-5	0.0
16	0.0	0.0	4.614970688013675E-5	9.22994137602735E-5	0.0
17	0.0	0.0	4.614970688013675E-5	1.3844912064041025E-4	0.0
18	0.0	0.0	4.614970688013675E-5	1.3844912064041025E-4	0.0
19	0.0	0.0	4.614970688013675E-5	1.84598827520547E-4	0.0
20	0.0	0.0	4.614970688013675E-5	2.3074853440068375E-4	0.0
21	0.0	0.0	4.614970688013675E-5	2.768982412808205E-4	0.0
22	0.0	0.0	4.614970688013675E-5	4.1534736192123077E-4	0.0
23	0.0	0.0	4.614970688013675E-5	5.999461894417778E-4	0.0
24	0.0	0.0	4.614970688013675E-5	8.306947238424615E-4	0.0
25	0.0	0.0	4.614970688013675E-5	0.0011537426720034188	0.0
26	0.0	0.0	4.614970688013675E-5	0.00184598827520547	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	5235	0.0	22.582333	4
CACGATT	135	0.0	22.516504	28
GTCCTAG	595	0.0	20.730824	1
CTATAGG	755	0.0	20.581053	1
TATAGGA	815	0.0	20.230255	2
TTCATCG	80	1.2679811E-7	19.998213	28
CGGTAGG	155	0.0	19.636528	1
TCTAGCG	155	0.0	19.611149	28
AGGACCT	8740	0.0	19.601486	5
TCCTATA	850	0.0	19.585567	2
GTCCTAT	940	0.0	19.42763	1
CGATTTT	190	0.0	19.36803	30
GGACCTG	8520	0.0	19.28199	6
TCACGAT	175	0.0	19.197397	27
AAGACGG	200	0.0	19.195183	5
TAGCGGC	160	0.0	18.999617	30
CATCGTT	135	1.8189894E-12	18.961267	28
TGTAGGA	15790	0.0	18.937233	2
CTCTAGG	705	0.0	18.632345	1
CTGTAGG	15525	0.0	18.542114	1
>>END_MODULE