FastQCFastQC Report
Thu 2 Feb 2017
SRR4062884_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062884_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166861
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA218211.007032753831464No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA167450.7727768417078898No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC161130.7436102269596434No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG158180.7299960634300031No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA94660.4368531253273745No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA83180.3838732618289775No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA80640.37215123628142277No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA72900.3364313631561969No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG62060.28640508089812866No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA57480.26526851514702604No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT55350.25543862758155694No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT52780.24357815291336177No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA52350.24159371551751588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT50500.2330560197446906No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA47020.216995921750403No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT43990.20301256056572156No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA43900.20259721320380034No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG43700.2016742190661976No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG43450.20052047639419418No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT41970.19369031977593396No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT40230.18566027077879016No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA38720.1786916650398895No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT37650.17375364640371485No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC36630.16904637630194091No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA36530.16858487923313956No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA34940.1612470758391978No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA34810.16064712964975603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34720.1602317822878348No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT33430.15427847010029716No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA31940.14740216377515677No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA30370.14015665979497532No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT28210.13018832310886577No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT28120.12977297574694455No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT28110.1297268260400644No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT28060.12949607750566372No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA26020.12008153730211582No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG24460.1128821830288145No Hit
CTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGT24450.11283603332193436No Hit
GATATACACTGTTCTACAATGCCGGTTTCCAACGTATG24080.1111284941673693No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCAC23750.10960555384032479No Hit
TCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG23530.10859026028896177No Hit
ACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA22960.10595972699679397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT21910.10111400777437962No Hit
GTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATG21680.10005256451613648No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG200.003752044232.003839
ACCCGGA350.002065689522.8598798
TAGGACC53400.022.5340124
TATAGGA9150.021.8575132
CTATAGG8450.020.8299351
GTGTAGG3900.020.514331
ATTGGCG555.8382215E-520.36278516
AGACCCG400.00448103520.0028555
TGCACCG400.00448103520.0028555
GACCGTT400.00448163320.0023947
GAGCGAC400.004485824619.99916321
TCGCGTA1052.7830538E-1019.8118959
CGCGTAA1052.8012437E-1019.81098210
ATAGGAC12150.019.7536283
CGTTTTG1550.019.61208330
ATTTAGA39750.019.362431
AGGACCT91450.019.2657385
CATCGTT2250.019.19919828
TTTAGAA42100.019.1160322
CGATTTT1351.8189894E-1218.9621730
Produced by FastQC (version 0.11.5)