##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062884_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2166861
Sequences flagged as poor quality	0
Sequence length	38
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.318325910152982	32.0	32.0	32.0	32.0	32.0
2	31.438836178232012	32.0	32.0	32.0	32.0	32.0
3	31.48426225770827	32.0	32.0	32.0	32.0	32.0
4	31.55859051411235	32.0	32.0	32.0	32.0	32.0
5	31.528961017804097	32.0	32.0	32.0	32.0	32.0
6	35.09241524952454	36.0	36.0	36.0	36.0	36.0
7	35.1250638596569	36.0	36.0	36.0	36.0	36.0
8	35.03241509261554	36.0	36.0	36.0	36.0	36.0
9	35.147823510598975	36.0	36.0	36.0	36.0	36.0
10	35.04251357147505	36.0	36.0	36.0	36.0	36.0
11	35.153170877135175	36.0	36.0	36.0	36.0	36.0
12	35.101104777833	36.0	36.0	36.0	36.0	36.0
13	35.120617335399	36.0	36.0	36.0	36.0	36.0
14	35.08813071073779	36.0	36.0	36.0	36.0	36.0
15	35.054355124763426	36.0	36.0	36.0	36.0	36.0
16	35.09180007393183	36.0	36.0	36.0	36.0	36.0
17	35.110410866225386	36.0	36.0	36.0	36.0	36.0
18	35.118765809158965	36.0	36.0	36.0	36.0	36.0
19	35.03852162182992	36.0	36.0	36.0	36.0	36.0
20	35.024312588578596	36.0	36.0	36.0	36.0	36.0
21	34.971888829048105	36.0	36.0	36.0	36.0	36.0
22	34.94186290675775	36.0	36.0	36.0	36.0	36.0
23	34.93891301749397	36.0	36.0	36.0	36.0	36.0
24	34.92561636394766	36.0	36.0	36.0	36.0	36.0
25	34.87959264576731	36.0	36.0	36.0	36.0	36.0
26	34.83956977397258	36.0	36.0	36.0	32.0	36.0
27	34.75121662164763	36.0	36.0	36.0	32.0	36.0
28	34.670649847867494	36.0	36.0	36.0	32.0	36.0
29	34.62971090439119	36.0	36.0	36.0	32.0	36.0
30	34.547912856431495	36.0	36.0	36.0	32.0	36.0
31	34.55016496212724	36.0	36.0	36.0	32.0	36.0
32	34.512914303224804	36.0	36.0	36.0	32.0	36.0
33	34.58224685385911	36.0	36.0	36.0	32.0	36.0
34	34.54589426825255	36.0	36.0	36.0	32.0	36.0
35	34.53034689350171	36.0	36.0	36.0	32.0	36.0
36	34.441934208054874	36.0	36.0	36.0	32.0	36.0
37	34.32285827286568	36.0	36.0	36.0	32.0	36.0
38	33.95618131481438	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	16.0
22	93.0
23	304.0
24	905.0
25	2422.0
26	5238.0
27	10900.0
28	20372.0
29	33683.0
30	54091.0
31	79587.0
32	115263.0
33	185280.0
34	485854.0
35	1172847.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.61874713266496	20.50007246222964	12.695151861448526	27.186028543656874
2	9.709944477287651	20.02361941998125	51.60990944966013	18.656526653070962
3	20.42442962423524	25.48696016957248	29.678738045495308	24.40987216069697
4	9.921614505816033	17.427885114601576	40.95447088060807	31.69602949897432
5	9.338309506986842	42.30510614673128	36.91919963412479	11.43738471215709
6	30.92676123677694	39.677542321647294	18.071142640634505	11.32455380094127
7	27.205621062833146	30.840851927913793	22.03101276045873	19.922514248794332
8	20.556505237196834	48.81161598074218	18.555174247095266	12.07670453496572
9	26.73256177262358	13.629044428660633	20.398315101184846	39.24007869753094
10	15.956284707816318	29.48806994889791	35.27539424941124	19.280251093874533
11	34.832613438276944	17.95854715193393	31.385655296801524	15.823184112987601
12	28.90223748449941	21.255802770192535	35.45731990125772	14.384639844050334
13	33.85974814235721	23.093288488083445	25.179949410644625	17.867013958914725
14	17.09980474059019	28.338273659454856	25.89353908718649	28.66838251276847
15	18.32023373903541	45.25629470464418	20.294287450833256	16.129184105487155
16	16.106024385516367	26.73541437840931	39.59803586756874	17.56052536850558
17	16.96546294386211	33.732666746967155	32.16856088138556	17.13330942778517
18	17.209456444137395	25.9309203497594	42.47762085339115	14.382002352712057
19	26.338606860338526	23.17855183142804	26.631934397268676	23.850906910964756
20	25.63030115914219	29.610528778726465	29.90948657989599	14.849683482235362
21	20.291971274523423	26.172239330975692	25.817901227446022	27.717888167054866
22	23.63669087989072	35.273252540542536	26.341157250583798	14.748899328982953
23	17.22507094370238	34.61300435330587	31.12394484366542	17.03797985932633
24	25.970101450900636	26.27667395370538	32.44412078116686	15.309103814227123
25	20.263330348984244	31.72696897815272	32.0292035479911	15.980497124871935
26	13.798300860092086	34.87274910573405	34.49598289876462	16.83296713540924
27	16.724815377842017	33.090354790207755	31.928442011428533	18.256387820521695
28	15.505009319933304	34.62049480792723	27.471951361900928	22.402544510238542
29	19.59696537987439	28.005949620210984	26.725987499890397	25.671097500024224
30	15.676270882165491	29.87058237699603	31.669867148838804	22.783279591999673
31	24.239579742309267	26.009744049110672	29.610298030192062	20.140378178387998
32	18.33495549553017	33.1266287962172	30.084301669557945	18.454114038694684
33	15.818550428476955	33.678117793434836	34.83282960928274	15.670502168805475
34	18.600131711263433	32.84123900887044	33.52651600633359	15.032113273532543
35	16.382638295672866	34.64024688247193	32.69462139011224	16.282493431742967
36	14.556217496184573	31.859173246461125	35.45266632238985	18.13194293496445
37	16.366855095919856	31.40261419629593	27.684378462670196	24.546152245114015
38	18.929741653821658	36.22601367877943	27.135763270354335	17.70848139704457
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21.0
1	38.0
2	55.0
3	55.0
4	227.0
5	399.0
6	399.0
7	681.0
8	963.0
9	1154.5
10	1346.0
11	1346.0
12	1785.5
13	2225.0
14	2934.5
15	3644.0
16	3644.0
17	5605.0
18	7566.0
19	7566.0
20	9490.5
21	11415.0
22	15782.0
23	20149.0
24	20149.0
25	37513.5
26	54878.0
27	54878.0
28	80714.0
29	106550.0
30	156692.0
31	206834.0
32	206834.0
33	259622.5
34	312411.0
35	312411.0
36	325693.5
37	338976.0
38	337798.0
39	336620.0
40	336620.0
41	279988.0
42	223356.0
43	174602.0
44	125848.0
45	125848.0
46	104770.0
47	83692.0
48	83692.0
49	76666.0
50	69640.0
51	65255.0
52	60870.0
53	60870.0
54	54692.5
55	48515.0
56	48515.0
57	45051.5
58	41588.0
59	35756.5
60	29925.0
61	29925.0
62	28630.5
63	27336.0
64	22605.5
65	17875.0
66	17875.0
67	15588.5
68	13302.0
69	13302.0
70	10881.5
71	8461.0
72	6587.0
73	4713.0
74	4713.0
75	3567.0
76	2421.0
77	2421.0
78	2220.0
79	2019.0
80	1716.0
81	1413.0
82	1413.0
83	1268.5
84	1124.0
85	1124.0
86	763.0
87	402.0
88	302.5
89	203.0
90	203.0
91	133.0
92	63.0
93	43.0
94	23.0
95	23.0
96	17.0
97	11.0
98	11.0
99	10.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009922186979229402
2	0.0
3	0.0
4	2.768982412808205E-4
5	9.22994137602735E-5
6	1.3844912064041025E-4
7	5.076467756815043E-4
8	0.0024459344646472476
9	0.003876575377931487
10	0.0010152935513630086
11	0.005076467756815043
12	3.69197655041094E-4
13	9.22994137602735E-5
14	0.0
15	0.0
16	4.614970688013675E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.84598827520547E-4
22	4.614970688013675E-5
23	9.22994137602735E-5
24	0.0
25	4.614970688013675E-5
26	0.0
27	1.3844912064041025E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	4.614970688013675E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2166861.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.284620034165144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.01508617687587	26.13040038655599
2	14.937365160146907	10.839932382950138
3	5.185656646741866	5.644787431640146
4	2.4208367254447682	3.513565629900639
5	1.3379924874798284	2.4274274508386515
6	0.8047982513699745	1.7521079253072034
7	0.5226892654736528	1.3275906975553855
8	0.3865951544810496	1.1221966629915407
9	0.291838456673896	0.9530322760592538
>10	1.7339214770143414	11.916657663898414
>50	0.18445527091507027	4.676608159552005
>100	0.14903532465476393	10.935282289892237
>500	0.016204547409638125	4.068792851443594
>1k	0.01173872725737565	8.363377687053708
>5k	0.0012759486149321359	3.064418993639929
>10k+	5.103794459728544E-4	3.2638215107211668
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	21821	1.007032753831464	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	16745	0.7727768417078898	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	16113	0.7436102269596434	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	15818	0.7299960634300031	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	9466	0.4368531253273745	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	8318	0.3838732618289775	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	8064	0.37215123628142277	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	7290	0.3364313631561969	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	6206	0.28640508089812866	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	5748	0.26526851514702604	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	5535	0.25543862758155694	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	5278	0.24357815291336177	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	5235	0.24159371551751588	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5050	0.2330560197446906	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	4702	0.216995921750403	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	4399	0.20301256056572156	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	4390	0.20259721320380034	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	4370	0.2016742190661976	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	4345	0.20052047639419418	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	4197	0.19369031977593396	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT	4023	0.18566027077879016	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA	3872	0.1786916650398895	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT	3765	0.17375364640371485	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC	3663	0.16904637630194091	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA	3653	0.16858487923313956	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	3494	0.1612470758391978	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA	3481	0.16064712964975603	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3472	0.1602317822878348	No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT	3343	0.15427847010029716	No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA	3194	0.14740216377515677	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA	3037	0.14015665979497532	No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT	2821	0.13018832310886577	No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT	2812	0.12977297574694455	No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT	2811	0.1297268260400644	No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT	2806	0.12949607750566372	No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA	2602	0.12008153730211582	No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG	2446	0.1128821830288145	No Hit
CTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGT	2445	0.11283603332193436	No Hit
GATATACACTGTTCTACAATGCCGGTTTCCAACGTATG	2408	0.1111284941673693	No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCAC	2375	0.10960555384032479	No Hit
TCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG	2353	0.10859026028896177	No Hit
ACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA	2296	0.10595972699679397	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2191	0.10111400777437962	No Hit
GTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATG	2168	0.10005256451613648	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	4.614970688013675E-5	0.0
13	0.0	0.0	0.0	4.614970688013675E-5	0.0
14	0.0	0.0	0.0	4.614970688013675E-5	0.0
15	0.0	0.0	0.0	4.614970688013675E-5	0.0
16	0.0	0.0	0.0	4.614970688013675E-5	0.0
17	0.0	0.0	0.0	9.22994137602735E-5	0.0
18	0.0	0.0	0.0	1.3844912064041025E-4	0.0
19	0.0	0.0	0.0	1.84598827520547E-4	0.0
20	0.0	0.0	0.0	2.3074853440068375E-4	0.0
21	0.0	0.0	0.0	2.3074853440068375E-4	0.0
22	0.0	0.0	0.0	4.1534736192123077E-4	0.0
23	0.0	0.0	0.0	6.922456032020512E-4	0.0
24	0.0	0.0	0.0	8.768444307225983E-4	0.0
25	0.0	0.0	0.0	9.691438444828718E-4	0.0
26	0.0	0.0	0.0	0.0013383414995239659	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	0.0037520442	32.00383	9
ACCCGGA	35	0.0020656895	22.859879	8
TAGGACC	5340	0.0	22.534012	4
TATAGGA	915	0.0	21.857513	2
CTATAGG	845	0.0	20.829935	1
GTGTAGG	390	0.0	20.51433	1
ATTGGCG	55	5.8382215E-5	20.362785	16
AGACCCG	40	0.004481035	20.002855	5
TGCACCG	40	0.004481035	20.002855	5
GACCGTT	40	0.004481633	20.002394	7
GAGCGAC	40	0.0044858246	19.999163	21
TCGCGTA	105	2.7830538E-10	19.811895	9
CGCGTAA	105	2.8012437E-10	19.810982	10
ATAGGAC	1215	0.0	19.753628	3
CGTTTTG	155	0.0	19.612083	30
ATTTAGA	3975	0.0	19.36243	1
AGGACCT	9145	0.0	19.265738	5
CATCGTT	225	0.0	19.199198	28
TTTAGAA	4210	0.0	19.116032	2
CGATTTT	135	1.8189894E-12	18.96217	30
>>END_MODULE