FastQCFastQC Report
Thu 2 Feb 2017
SRR4062883_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062883_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486448
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT19610.4031263362168207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT14610.30034042693155283No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT13870.28512811235733315No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13540.2783442423445055No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13530.27813867052593494No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13340.27423280597309474No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13140.27012136960168404No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12660.2602539223102983No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12650.2600483504917278No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11850.2436026050060849No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11690.24031345590895636No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC11240.23106272407328227No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10950.2251011413347367No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC10920.22448442587902512No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10860.2232509949676019No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT10700.2199618458704733No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG10500.2158504094990626No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT10270.21112225767194026No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG10170.2090665394862349No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA9740.20022695128770188No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA9650.19837680492056706No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT9440.1940597967305858No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA9320.19159293490773938No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT9160.1883037858106108No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT8770.1802864848863599No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC8720.17925862579350724No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT8680.17843633851922508No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC8620.17720290760780188No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC8340.17144689668782687No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC8270.1700078939578331No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC8110.16671874486070454No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC7990.16425188303785812No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT7940.16322402394500543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT7840.16116830575930008No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG7710.1584958721178831No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT7630.15685129756931881No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC7500.15417886392790184No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC7460.1533565766536197No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA7410.15232871756076702No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7310.15027299937506167No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA7280.14965628391935007No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG6890.14163898299509917No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG6780.13937769299082325No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT6710.13793869026082953No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT6550.13464954116370095No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG6440.13238825115942507No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA6310.1297158175180081No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT6300.12951024569943756No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC6220.12786567115087327No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTC6170.12683781205802058No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC6130.12601552478373845No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT6100.12539880932802686No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAA5990.12313751932375094No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG5990.12313751932375094No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGT5930.12190408841232772No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCC5910.12149294477518666No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC5750.1182037956780581No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAG5680.11676479294806434No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCAT5660.11635364931092326No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG5570.11450350294378844No Hit
TCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAA5350.10998092293523665No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT5250.1079252047495313No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC5220.10730848929381968No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT5160.10607505838239648No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACC5130.10545834292668486No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGC5110.1050471992895438No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGG5110.1050471992895438No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAA5090.10463605565240272No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTC5070.10422491201526164No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG5070.10422491201526164No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA5050.10381376837812059No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA5010.10299148110383843No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG4940.10155247837384468No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCC4940.10155247837384468No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGC4930.10134690655527415No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAG4900.10073019109956255No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC4890.10052461928099202No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCCG200.003750165232.002169
TCGCCGA401.5909808E-424.0016212
CCGACGT401.5909808E-424.0016215
GTAAGAG350.00206773222.8516373
ACGTCTC502.796308E-522.39690618
CTCGCCG453.5311078E-421.33477411
GCCCTCG555.830631E-520.3608238
TAAGCCA801.2608507E-720.0013522
ACTGATG400.004483409719.9972368
GGGCTGT400.004486078419.995186
CTGGTTC751.5039404E-619.20129625
ATTGTCG507.1994413E-419.1953746
CCCTCGC601.1358798E-418.6679279
GGCCCTC601.13767295E-418.6640877
GTCCTAT3200.018.5088621
GGACCTG1600.017.9956636
GTCCTAA1700.017.8909191
TGAAATC450.00883970317.77897820
ACGATTA450.00884494117.7771532
GTACACC450.00885542617.7734954
Produced by FastQC (version 0.11.5)