##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062883_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	486448
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.32154721573529	32.0	32.0	32.0	21.0	32.0
2	29.484522497779825	32.0	32.0	32.0	14.0	32.0
3	29.601782718810643	32.0	32.0	32.0	21.0	32.0
4	29.35288047232181	32.0	32.0	32.0	21.0	32.0
5	29.45878696181298	32.0	32.0	32.0	21.0	32.0
6	32.73848797816005	36.0	32.0	36.0	21.0	36.0
7	32.73358920172351	36.0	32.0	36.0	21.0	36.0
8	32.63003445383679	36.0	32.0	36.0	21.0	36.0
9	32.86547791336381	36.0	32.0	36.0	21.0	36.0
10	31.99083355260994	36.0	32.0	36.0	21.0	36.0
11	33.127117389731275	36.0	32.0	36.0	21.0	36.0
12	32.49016544419958	36.0	32.0	36.0	21.0	36.0
13	32.824690408841235	36.0	32.0	36.0	21.0	36.0
14	32.363436585205406	36.0	32.0	36.0	21.0	36.0
15	32.29659901983357	36.0	32.0	36.0	21.0	36.0
16	32.42768188994507	36.0	32.0	36.0	21.0	36.0
17	32.13899121797191	36.0	32.0	36.0	21.0	36.0
18	32.33691370917344	36.0	32.0	36.0	21.0	36.0
19	32.23058785317239	36.0	32.0	36.0	21.0	36.0
20	32.17638884320626	36.0	32.0	36.0	21.0	36.0
21	32.17888859652008	36.0	32.0	36.0	21.0	36.0
22	32.11485297503536	36.0	32.0	36.0	14.0	36.0
23	32.016166167812386	36.0	32.0	36.0	14.0	36.0
24	32.03670484820577	36.0	32.0	36.0	14.0	36.0
25	32.04977099299411	36.0	32.0	36.0	14.0	36.0
26	31.927254300562446	36.0	32.0	36.0	14.0	36.0
27	32.00622471466632	36.0	32.0	36.0	14.0	36.0
28	31.93241620892675	36.0	32.0	36.0	14.0	36.0
29	31.914344390356217	36.0	32.0	36.0	14.0	36.0
30	31.81992936552314	36.0	32.0	36.0	14.0	36.0
31	31.856163454264383	36.0	32.0	36.0	14.0	36.0
32	31.75404565338947	36.0	32.0	36.0	14.0	36.0
33	31.71139155675427	36.0	32.0	36.0	14.0	36.0
34	31.74031551162714	36.0	32.0	36.0	14.0	36.0
35	31.7731103838437	36.0	32.0	36.0	14.0	36.0
36	31.657081538006118	36.0	32.0	36.0	14.0	36.0
37	31.76402410946288	36.0	32.0	36.0	14.0	36.0
38	30.58508411998816	36.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	4.0
10	5.0
11	2.0
12	2.0
13	3.0
14	268.0
15	569.0
16	804.0
17	1179.0
18	1529.0
19	2018.0
20	2603.0
21	3373.0
22	4355.0
23	5636.0
24	7487.0
25	9838.0
26	12875.0
27	16467.0
28	21051.0
29	26923.0
30	33621.0
31	42241.0
32	52964.0
33	68988.0
34	95527.0
35	76112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.40842554172429	18.93297602581835	11.830871698610288	24.827726733847065
2	16.512025406280404	20.94208100308734	37.508613082835744	25.03728050779651
3	17.83285361842105	26.31352796052632	28.99588815789474	26.857730263157897
4	11.563241358610963	16.132785678750427	37.432314087304036	34.871658875334575
5	14.008691576489163	37.46690293721014	33.07136631253495	15.453039173765749
6	31.795998750123346	37.54687037463408	17.580707495970792	13.076423379271782
7	29.245058053481564	31.355047199289544	21.87325263954215	17.52664210768674
8	28.421301483209817	31.299735838584013	20.31925500313499	19.95970767507118
9	28.125629566282797	13.766173429322063	19.77197880134691	38.33621820304823
10	17.36705561174565	24.55719466166712	29.756354277162426	28.319395449424807
11	37.519838169872294	21.51444383228215	22.76291228445263	18.202805713392923
12	25.42837348617384	24.69589851017509	27.92697041524732	21.948757588403748
13	28.387413800902184	19.209086011998995	26.428016300213415	25.975483886885403
14	24.06610170188445	21.60126808689109	24.16314077655931	30.169489434665152
15	24.931373899669154	27.79547263078027	22.94630611661605	24.326847352934525
16	25.747339594401225	25.49116761788845	24.680093422589184	24.081399365121136
17	23.29205956253367	26.02941115995608	25.60296578978752	25.075563487722736
18	25.25528297503603	23.046907925439076	28.10363861757613	23.594170481948765
19	25.683071343692305	25.03196917801179	25.950748962800624	23.334210515495286
20	25.939220499409966	23.041688767181864	25.49906047112131	25.52003026228686
21	28.518475878770932	23.426559050035156	23.73061852151853	24.324346549675383
22	26.6297018141149	24.247339681913125	24.952714593509974	24.170243910462
23	23.148485091457836	23.751899160976894	26.709142082940136	26.39047366462513
24	24.396296669428608	25.471397466553448	25.778157915838257	24.354147948179687
25	24.385896015083507	24.19899703716802	25.838115466147432	25.57699148160104
26	23.273520564366066	26.310421896942742	27.17913605605828	23.236921482632912
27	25.87880706746327	24.53546272805398	24.91622087261872	24.669509331864028
28	23.250640905717475	24.39841207513656	27.094430544733882	25.256516474412084
29	23.56840194326856	24.58650032792143	26.853167268712596	24.99193046009741
30	23.22405438798103	25.075087730839524	27.788936149217463	23.911921731961986
31	24.858762869642444	24.656055721851256	24.725954738330977	25.759226670175323
32	23.948060385147542	24.986071449864415	24.66535501869818	26.400513146289867
33	22.91191236878138	24.267786727850627	26.616255689738118	26.20404521362988
34	24.789772563726157	24.425446874653048	26.999782062511052	23.784998499109747
35	26.37892252209278	24.016670847400476	26.2713885656245	23.333018064882246
36	23.35722451127943	25.4944774781443	25.625241584630693	25.523056425945573
37	25.79800903786611	25.540195477777456	24.79903289076024	23.86276259359619
38	23.42908714878675	24.92347164287726	26.169511595922053	25.477929612413934
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	266.0
1	250.5
2	235.0
3	235.0
4	498.0
5	761.0
6	761.0
7	896.0
8	1031.0
9	909.0
10	787.0
11	787.0
12	901.5
13	1016.0
14	1349.5
15	1683.0
16	1683.0
17	2404.5
18	3126.0
19	3126.0
20	3496.0
21	3866.0
22	4095.5
23	4325.0
24	4325.0
25	4878.0
26	5431.0
27	5431.0
28	7117.5
29	8804.0
30	11020.0
31	13236.0
32	13236.0
33	17986.0
34	22736.0
35	22736.0
36	23556.0
37	24376.0
38	27304.0
39	30232.0
40	30232.0
41	29500.5
42	28769.0
43	33728.0
44	38687.0
45	38687.0
46	37312.0
47	35937.0
48	35937.0
49	38130.5
50	40324.0
51	40339.0
52	40354.0
53	40354.0
54	37183.5
55	34013.0
56	34013.0
57	34475.0
58	34937.0
59	30269.0
60	25601.0
61	25601.0
62	27439.5
63	29278.0
64	24100.5
65	18923.0
66	18923.0
67	16395.5
68	13868.0
69	13868.0
70	11450.5
71	9033.0
72	6805.5
73	4578.0
74	4578.0
75	3512.0
76	2446.0
77	2446.0
78	2720.0
79	2994.0
80	2312.0
81	1630.0
82	1630.0
83	1837.5
84	2045.0
85	2045.0
86	1231.5
87	418.0
88	385.0
89	352.0
90	352.0
91	216.0
92	80.0
93	66.0
94	52.0
95	52.0
96	45.0
97	38.0
98	38.0
99	109.0
100	180.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12169851659375719
2	0.05529881919547413
3	0.009867447291385719
4	0.002055718185705358
5	0.0
6	0.0
7	0.0
8	6.167154557116074E-4
9	4.111436371410716E-4
10	8.222872742821432E-4
11	0.0024668618228464298
12	0.0030835772785580374
13	0.015212314574219649
14	0.009456303654244647
15	0.024051902772752688
16	0.011512021839950004
17	0.020968325494194653
18	0.005550439101404466
19	0.008634016379962504
20	0.0069894418313982175
21	0.006167154557116075
22	0.008222872742821432
23	0.010073019109956255
24	0.015417886392790186
25	0.018295891852777687
26	0.01994046640134197
27	0.01027859092852679
28	0.005550439101404466
29	0.010484162747097327
30	0.0034947209156991088
31	0.006578298194257146
32	0.007606157287109824
33	0.009456303654244647
34	0.014390027299937507
35	0.018501463671348223
36	0.01562345821136072
37	0.01027859092852679
38	0.005550439101404466
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	486448.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.57485543715401
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.6897992340893	31.446321077853483
2	9.457676727579663	7.107416716202833
3	2.323910791855914	2.6196183615848416
4	0.9920164692289781	1.4909950169021917
5	0.5099706078893296	0.9581035934319556
6	0.3595900365833604	0.8106926184756416
7	0.25071701983876277	0.659445904325291
8	0.20464309833016134	0.6151547868773686
9	0.1740847956700766	0.588708992799866
>10	1.4269430709511408	11.31950138235142
>50	0.2752832438135796	7.128552609081787
>100	0.29603872648206375	22.988935681283102
>500	0.028948436353412137	7.42100920688053
>1k	0.010377741334242088	4.8455440519497035
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1961	0.4031263362168207	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1461	0.30034042693155283	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1387	0.28512811235733315	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1354	0.2783442423445055	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1353	0.27813867052593494	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1334	0.27423280597309474	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1314	0.27012136960168404	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1266	0.2602539223102983	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1265	0.2600483504917278	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1185	0.2436026050060849	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1169	0.24031345590895636	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1124	0.23106272407328227	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1095	0.2251011413347367	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1092	0.22448442587902512	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1086	0.2232509949676019	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1070	0.2199618458704733	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1050	0.2158504094990626	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1027	0.21112225767194026	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1017	0.2090665394862349	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	974	0.20022695128770188	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	965	0.19837680492056706	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	944	0.1940597967305858	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	932	0.19159293490773938	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	916	0.1883037858106108	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	877	0.1802864848863599	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	872	0.17925862579350724	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	868	0.17843633851922508	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	862	0.17720290760780188	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	834	0.17144689668782687	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	827	0.1700078939578331	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	811	0.16671874486070454	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	799	0.16425188303785812	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	794	0.16322402394500543	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	784	0.16116830575930008	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	771	0.1584958721178831	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	763	0.15685129756931881	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	750	0.15417886392790184	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	746	0.1533565766536197	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA	741	0.15232871756076702	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	731	0.15027299937506167	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	728	0.14965628391935007	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	689	0.14163898299509917	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	678	0.13937769299082325	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	671	0.13793869026082953	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	655	0.13464954116370095	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	644	0.13238825115942507	No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA	631	0.1297158175180081	No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT	630	0.12951024569943756	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	622	0.12786567115087327	No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTC	617	0.12683781205802058	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC	613	0.12601552478373845	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT	610	0.12539880932802686	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAA	599	0.12313751932375094	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	599	0.12313751932375094	No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGT	593	0.12190408841232772	No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCC	591	0.12149294477518666	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC	575	0.1182037956780581	No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAG	568	0.11676479294806434	No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCAT	566	0.11635364931092326	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	557	0.11450350294378844	No Hit
TCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAA	535	0.10998092293523665	No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	525	0.1079252047495313	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	522	0.10730848929381968	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT	516	0.10607505838239648	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACC	513	0.10545834292668486	No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGC	511	0.1050471992895438	No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGG	511	0.1050471992895438	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAA	509	0.10463605565240272	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTC	507	0.10422491201526164	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG	507	0.10422491201526164	No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA	505	0.10381376837812059	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA	501	0.10299148110383843	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG	494	0.10155247837384468	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCC	494	0.10155247837384468	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGC	493	0.10134690655527415	No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAG	490	0.10073019109956255	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	489	0.10052461928099202	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	2.055718185705358E-4	0.0	0.0	0.0	0.0
12	2.055718185705358E-4	0.0	0.0	0.0	0.0
13	2.055718185705358E-4	0.0	0.0	0.0	0.0
14	2.055718185705358E-4	0.0	0.0	0.0	0.0
15	2.055718185705358E-4	0.0	0.0	0.0	0.0
16	2.055718185705358E-4	0.0	0.0	0.0	0.0
17	2.055718185705358E-4	0.0	0.0	0.0	0.0
18	2.055718185705358E-4	0.0	0.0	0.0	0.0
19	2.055718185705358E-4	0.0	0.0	0.0	0.0
20	2.055718185705358E-4	0.0	0.0	0.0	0.0
21	2.055718185705358E-4	0.0	0.0	0.0	0.0
22	2.055718185705358E-4	0.0	0.0	0.0	0.0
23	2.055718185705358E-4	0.0	0.0	4.111436371410716E-4	0.0
24	2.055718185705358E-4	0.0	0.0	0.001027859092852679	0.0
25	2.055718185705358E-4	0.0	0.0	0.001027859092852679	0.0
26	2.055718185705358E-4	0.0	0.0	0.0014390027299937506	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACCCG	20	0.0037501652	32.00216	9
TCGCCGA	40	1.5909808E-4	24.00162	12
CCGACGT	40	1.5909808E-4	24.00162	15
GTAAGAG	35	0.002067732	22.851637	3
ACGTCTC	50	2.796308E-5	22.396906	18
CTCGCCG	45	3.5311078E-4	21.334774	11
GCCCTCG	55	5.830631E-5	20.360823	8
TAAGCCA	80	1.2608507E-7	20.00135	22
ACTGATG	40	0.0044834097	19.997236	8
GGGCTGT	40	0.0044860784	19.99518	6
CTGGTTC	75	1.5039404E-6	19.201296	25
ATTGTCG	50	7.1994413E-4	19.195374	6
CCCTCGC	60	1.1358798E-4	18.667927	9
GGCCCTC	60	1.13767295E-4	18.664087	7
GTCCTAT	320	0.0	18.508862	1
GGACCTG	160	0.0	17.995663	6
GTCCTAA	170	0.0	17.890919	1
TGAAATC	45	0.008839703	17.778978	20
ACGATTA	45	0.008844941	17.77715	32
GTACACC	45	0.008855426	17.773495	4
>>END_MODULE