FastQCFastQC Report
Thu 2 Feb 2017
SRR4062883_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062883_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486448
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT17250.3546113870341743No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16120.3313817715357037No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG13660.2808111041673519No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13600.2795776732559287No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13250.2723826596059599No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13080.26888793869026084No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA13080.26888793869026084No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13030.26786007959740815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT12910.2653932177745617No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12730.26169292504029207No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC12710.261281781403151No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC12520.2573759168503108No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12480.2565536295760287No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT12260.2520310495674769No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10760.22119527678189652No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10410.2140002631319278No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10370.21317797585764564No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC10050.2065996776633885No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT9960.20474953129625365No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG9520.19570437127915005No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT9420.19364865309344473No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT9280.19077064763345722No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT9260.19035950399631615No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9240.1899483603591751No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT8930.18357563398348847No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC8590.17658619215209026No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG8280.17021346577640364No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC8070.16589645758642238No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT7940.16322402394500543No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA7820.160757162122159No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC7730.15890701575502417No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG7610.15644015393217775No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA7570.1556178666578956No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC7450.15315100483504918No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC7380.15171200210505542No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG7370.1515064302864849No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG7300.15006742755649113No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT7190.14780613755221522No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC7180.14760056573364472No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG7030.14451698845508668No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC7030.14451698845508668No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC7010.1441058448179456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT6900.1418445548136697No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT6660.13691083116797687No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC6580.13526625661941255No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCAT6470.13300496661513667No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG6390.13136039206657238No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA6380.13115482024800185No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAG6360.13074367661086078No Hit
TCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAA6360.13074367661086078No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTC6320.12992138933657862No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG6310.1297158175180081No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC6150.12642666842087952No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA6110.12560438114659736No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGT6110.12560438114659736No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT6090.12519323750945632No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5830.11984837022662237No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA5820.11964279840805184No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACC5810.1194372265894813No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGG5690.11697036476663487No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTC5680.11676479294806434No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAA5580.11470907476235898No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCAT5580.11470907476235898No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT5580.11470907476235898No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCC5530.1136812156695063No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAA5480.11265335657665361No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG5450.112036641120942No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT5440.11183106930237148No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAAC5410.11121435384665987No Hit
ACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTAT5240.10771963293096076No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCC5220.10730848929381968No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC5170.106280630200967No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT5150.10586948656382594No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGG5120.10525277110811433No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA5050.10381376837812059No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGC4950.10175805019241521No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTT4950.10175805019241521No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG4940.10155247837384468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAAT308.410164E-426.66649631
GACATAT401.5895606E-424.004781
GCGACGC401.5917793E-423.99984632
CTACACG350.002064915422.8569954
CACCGGT350.002064915422.8569957
TAAGACC350.002064915422.8569954
CAAGTTA854.292815E-1022.5880922
TCTAGCG2500.022.39985728
CTAGCGG2550.021.96064429
TCTTGCA453.5328735E-421.3331952
TAGCGGC2750.020.36350630
TTAATAC400.00447999419.9998723
GATATAC659.3992985E-619.696231
TTATAAC659.41601E-619.692182
AGCGGCG2850.019.64899631
CAACGGA902.0312655E-819.5554314
TTACCCT902.0312655E-819.5554326
CGCGCTG507.1880856E-419.19987716
ATATAGG507.1880856E-419.1998774
TTGCACC507.1880856E-419.1998774
Produced by FastQC (version 0.11.5)