##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062883_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	486448
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.840239861197908	32.0	32.0	32.0	32.0	32.0
2	30.704749531296255	32.0	32.0	32.0	32.0	32.0
3	31.05456698352136	32.0	32.0	32.0	32.0	32.0
4	31.260105910600927	32.0	32.0	32.0	32.0	32.0
5	31.07440055257705	32.0	32.0	32.0	32.0	32.0
6	34.62626837812058	36.0	36.0	36.0	32.0	36.0
7	34.658738446863794	36.0	36.0	36.0	32.0	36.0
8	34.56870210176627	36.0	36.0	36.0	32.0	36.0
9	34.77172688550472	36.0	36.0	36.0	32.0	36.0
10	34.49785588593231	36.0	36.0	36.0	32.0	36.0
11	34.819775186659214	36.0	36.0	36.0	32.0	36.0
12	34.60231514982074	36.0	36.0	36.0	32.0	36.0
13	34.738767555833306	36.0	36.0	36.0	32.0	36.0
14	34.633444890306876	36.0	36.0	36.0	32.0	36.0
15	34.59526198072559	36.0	36.0	36.0	32.0	36.0
16	34.5984236752952	36.0	36.0	36.0	32.0	36.0
17	34.557599167845275	36.0	36.0	36.0	32.0	36.0
18	34.57529478998783	36.0	36.0	36.0	32.0	36.0
19	34.55499868434036	36.0	36.0	36.0	32.0	36.0
20	34.52275680031576	36.0	36.0	36.0	32.0	36.0
21	34.52202907607802	36.0	36.0	36.0	32.0	36.0
22	34.50193031937638	36.0	36.0	36.0	32.0	36.0
23	34.43021659046805	36.0	36.0	36.0	32.0	36.0
24	34.39969328684669	36.0	36.0	36.0	32.0	36.0
25	34.37689126073085	36.0	36.0	36.0	32.0	36.0
26	34.3122409795086	36.0	36.0	36.0	32.0	36.0
27	34.30620744663356	36.0	36.0	36.0	32.0	36.0
28	34.2397748577443	36.0	36.0	36.0	32.0	36.0
29	34.17362390224649	36.0	36.0	36.0	32.0	36.0
30	34.154846972338255	36.0	36.0	36.0	32.0	36.0
31	34.16339875999079	36.0	36.0	36.0	32.0	36.0
32	34.12480676249054	36.0	36.0	36.0	32.0	36.0
33	34.085306137552216	36.0	36.0	36.0	32.0	36.0
34	34.04971754432128	36.0	36.0	36.0	32.0	36.0
35	34.03753741407098	36.0	36.0	36.0	32.0	36.0
36	33.95682991810019	36.0	36.0	36.0	32.0	36.0
37	33.95396424694931	36.0	36.0	36.0	32.0	36.0
38	33.35584892938197	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	10.0
22	37.0
23	140.0
24	307.0
25	804.0
26	1778.0
27	3500.0
28	6472.0
29	10536.0
30	16944.0
31	25995.0
32	40044.0
33	66750.0
34	140586.0
35	172543.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.39158895412904	20.737117810562324	12.707644611108371	25.163648624200256
2	15.616674341347894	22.28439627668322	36.52887872907279	25.570050652896096
3	16.791517284478505	27.453705226457913	29.273426964444297	26.48135052461928
4	11.206130973917046	17.06903923954873	37.930467388086704	33.79436239844752
5	13.79016873334868	37.93827911719238	32.315067592013946	15.95648455744499
6	32.03014505147518	38.27562247146663	16.73539617800875	12.958836299049437
7	28.86301871122386	31.826348659460656	21.677226249162292	17.633406380153193
8	28.18294698583164	31.887236964369414	19.932529664745207	19.99728638505374
9	28.27583654891835	13.416236151741352	19.404316730156054	38.903610569184245
10	17.187554605351067	24.799720420602533	29.491720543946386	28.52100443010001
11	37.78434262759827	21.502829806611036	22.56321066383789	18.149616901952804
12	25.186248067624906	24.99958885636286	27.668116633226987	22.146046442785252
13	28.50541065026478	19.232477058185047	26.04204354833405	26.220068743216128
14	24.11855762292706	21.55198819193047	23.96026699722683	30.369187187915642
15	25.099085616550997	28.063225668519554	22.34956254317008	24.488126171759365
16	25.95179751998158	25.79124592967799	23.94048284708746	24.31647370325297
17	23.451016347071015	26.292635595171532	24.969164227214417	25.287183830543036
18	25.704083478604083	23.368170904187085	27.50098674472914	23.426758872479688
19	25.91993388810315	25.27937210143736	25.46500345360655	23.335690556852942
20	26.079868762951026	23.11572871098247	25.130538104792286	25.673864421274217
21	28.68754728151827	23.650832154721574	23.398184389698386	24.26343617406177
22	26.491834687366378	24.713638456731243	24.651966911160084	24.142559944742295
23	23.12744630464099	23.83790250962076	26.442086307272312	26.59256487846594
24	24.51567279544782	25.77562247146663	25.321925467881456	24.38677926520409
25	24.41699832253396	24.48216458902082	25.571489655626088	25.529347432819126
26	23.586282603690425	26.169292504029208	26.84994079531625	23.394484096964117
27	25.961048251817253	24.57323290464757	24.5325296845706	24.933189158964574
28	23.341446567772916	24.554525869157647	27.070519356642436	25.033508206426998
29	23.768213663125348	24.4829868762951	26.664720586784195	25.084078873795352
30	23.485552412590863	24.942851034437393	27.475906982863535	24.095689570108213
31	25.003905864552838	24.73028977403546	24.35943821333421	25.906366148077492
32	24.406308587968294	25.08058415287965	24.22458310035194	26.28852415880012
33	23.195490576587837	24.319351708712954	26.240625925073186	26.244531789626024
34	24.982115251784364	24.448450810775253	26.582697431174555	23.986736506265828
35	26.95293227642009	24.066087228234057	25.577862382001776	23.403118113344078
36	23.76492451402822	25.47980462454363	25.241341315001808	25.51392954642634
37	26.328199519784235	25.715389928625466	24.003387823570044	23.95302272802026
38	24.030728875439923	24.75393053317107	25.74519784231819	25.470142749070817
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	167.0
1	130.0
2	93.0
3	93.0
4	261.5
5	430.0
6	430.0
7	567.5
8	705.0
9	711.0
10	717.0
11	717.0
12	863.5
13	1010.0
14	1254.0
15	1498.0
16	1498.0
17	2173.0
18	2848.0
19	2848.0
20	3190.0
21	3532.0
22	3862.5
23	4193.0
24	4193.0
25	4773.0
26	5353.0
27	5353.0
28	7180.5
29	9008.0
30	11086.0
31	13164.0
32	13164.0
33	18113.0
34	23062.0
35	23062.0
36	23712.5
37	24363.0
38	27311.5
39	30260.0
40	30260.0
41	29579.5
42	28899.0
43	33612.0
44	38325.0
45	38325.0
46	37503.5
47	36682.0
48	36682.0
49	38639.0
50	40596.0
51	40352.5
52	40109.0
53	40109.0
54	37173.0
55	34237.0
56	34237.0
57	34815.5
58	35394.0
59	30879.0
60	26364.0
61	26364.0
62	28143.5
63	29923.0
64	24447.0
65	18971.0
66	18971.0
67	16609.0
68	14247.0
69	14247.0
70	11710.5
71	9174.0
72	6865.0
73	4556.0
74	4556.0
75	3368.0
76	2180.0
77	2180.0
78	2381.0
79	2582.0
80	2011.5
81	1441.0
82	1441.0
83	1447.5
84	1454.0
85	1454.0
86	971.0
87	488.0
88	402.0
89	316.0
90	316.0
91	191.5
92	67.0
93	44.5
94	22.0
95	22.0
96	14.0
97	6.0
98	6.0
99	9.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008222872742821432
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	4.111436371410716E-4
8	0.0024668618228464298
9	0.0030835772785580374
10	6.167154557116074E-4
11	0.00390586455284018
12	0.0
13	0.0
14	2.055718185705358E-4
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	486448.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.476381965004244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.77048354431699	30.191677731906047
2	9.993509748399488	7.290541575072264
3	2.4227864887389092	2.651234561486757
4	1.0600154818960021	1.5466211842582647
5	0.6006854860797984	1.0955416615540483
6	0.3534483639673631	0.7735510517387628
7	0.2780887832214254	0.7100570873877584
8	0.20683204003768685	0.6035587596012573
9	0.18327760258119577	0.601677345364375
>10	1.4978277845888952	11.62508293316488
>50	0.2874395541227968	7.292157379633776
>100	0.30325155317080926	22.89961119442634
>500	0.032188712347739526	7.9754173448394265
>1k	0.010164856530865114	4.743270189566028
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1725	0.3546113870341743	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1612	0.3313817715357037	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1366	0.2808111041673519	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1360	0.2795776732559287	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1325	0.2723826596059599	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1308	0.26888793869026084	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1308	0.26888793869026084	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1303	0.26786007959740815	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1291	0.2653932177745617	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1273	0.26169292504029207	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1271	0.261281781403151	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1252	0.2573759168503108	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1248	0.2565536295760287	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1226	0.2520310495674769	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1076	0.22119527678189652	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1041	0.2140002631319278	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1037	0.21317797585764564	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1005	0.2065996776633885	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	996	0.20474953129625365	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	952	0.19570437127915005	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	942	0.19364865309344473	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	928	0.19077064763345722	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	926	0.19035950399631615	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	924	0.1899483603591751	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	893	0.18357563398348847	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	859	0.17658619215209026	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	828	0.17021346577640364	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	807	0.16589645758642238	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	794	0.16322402394500543	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA	782	0.160757162122159	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC	773	0.15890701575502417	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	761	0.15644015393217775	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	757	0.1556178666578956	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	745	0.15315100483504918	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC	738	0.15171200210505542	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	737	0.1515064302864849	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	730	0.15006742755649113	No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT	719	0.14780613755221522	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	718	0.14760056573364472	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	703	0.14451698845508668	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	703	0.14451698845508668	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	701	0.1441058448179456	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	690	0.1418445548136697	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	666	0.13691083116797687	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	658	0.13526625661941255	No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCAT	647	0.13300496661513667	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	639	0.13136039206657238	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	638	0.13115482024800185	No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAG	636	0.13074367661086078	No Hit
TCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAA	636	0.13074367661086078	No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTC	632	0.12992138933657862	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG	631	0.1297158175180081	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	615	0.12642666842087952	No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA	611	0.12560438114659736	No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGT	611	0.12560438114659736	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	609	0.12519323750945632	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	583	0.11984837022662237	No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA	582	0.11964279840805184	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACC	581	0.1194372265894813	No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGG	569	0.11697036476663487	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTC	568	0.11676479294806434	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAA	558	0.11470907476235898	No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCAT	558	0.11470907476235898	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT	558	0.11470907476235898	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCC	553	0.1136812156695063	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAA	548	0.11265335657665361	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	545	0.112036641120942	No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	544	0.11183106930237148	No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAAC	541	0.11121435384665987	No Hit
ACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTAT	524	0.10771963293096076	No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCC	522	0.10730848929381968	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	517	0.106280630200967	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	515	0.10586948656382594	No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGG	512	0.10525277110811433	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA	505	0.10381376837812059	No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGC	495	0.10175805019241521	No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTT	495	0.10175805019241521	No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG	494	0.10155247837384468	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	6.167154557116074E-4	0.0
24	0.0	0.0	0.0	0.0012334309114232149	0.0
25	0.0	0.0	0.0	0.0012334309114232149	0.0
26	0.0	0.0	0.0	0.0016445745485642864	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGAAT	30	8.410164E-4	26.666496	31
GACATAT	40	1.5895606E-4	24.00478	1
GCGACGC	40	1.5917793E-4	23.999846	32
CTACACG	35	0.0020649154	22.856995	4
CACCGGT	35	0.0020649154	22.856995	7
TAAGACC	35	0.0020649154	22.856995	4
CAAGTTA	85	4.292815E-10	22.58809	22
TCTAGCG	250	0.0	22.399857	28
CTAGCGG	255	0.0	21.960644	29
TCTTGCA	45	3.5328735E-4	21.333195	2
TAGCGGC	275	0.0	20.363506	30
TTAATAC	40	0.004479994	19.999872	3
GATATAC	65	9.3992985E-6	19.69623	1
TTATAAC	65	9.41601E-6	19.69218	2
AGCGGCG	285	0.0	19.648996	31
CAACGGA	90	2.0312655E-8	19.55543	14
TTACCCT	90	2.0312655E-8	19.55543	26
CGCGCTG	50	7.1880856E-4	19.199877	16
ATATAGG	50	7.1880856E-4	19.199877	4
TTGCACC	50	7.1880856E-4	19.199877	4
>>END_MODULE