FastQCFastQC Report
Thu 2 Feb 2017
SRR4062882_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062882_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109691
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31650.2852145326942365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21170.19077382802960463No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15720.1416610569969478No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15570.14030932935384716No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14780.13319023043351708No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14690.13237919384765667No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14630.13183850279041642No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14210.12805366538973462No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13700.12345779140319244No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13650.12300721552215886No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13320.12003341470733744No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13150.11850145671182337No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12580.11336489166804092No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA11490.10354233746150956No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA11260.10146968840875524No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11200.10092899735151498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11190.10083888217530827No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT11180.10074876699910154No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCCG350.002067985522.854315
AACCCGT1006.816117E-817.59940529
ACCCGTT1006.819573E-817.59861230
TAACCCG1101.0633812E-817.45395528
CGAATGT854.9852188E-616.94060523
TAACGAA1451.2205419E-915.44844913
GATATAT1603.0013325E-1015.0123471
CAAGACG4050.015.0111614
TCTAGCG3550.014.87273828
ATAACGA1401.2778401E-814.8573112
CATCGTT2600.014.76873128
ATACGCG650.004163641514.7680654
CTAATCT650.004163641514.7680654
AAGACGG3900.014.7674015
ACCGTCG2950.014.6422538
CTAGCGG3650.014.46526329
TAGCGGC3650.014.46461230
GTATTAG3100.014.4635091
GTAGACT1002.3272796E-514.3988654
CTAATTG1002.3272796E-514.39886530
Produced by FastQC (version 0.11.5)