##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062882_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1109691
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17830008533907	32.0	32.0	32.0	32.0	32.0
2	30.71716450795762	32.0	32.0	32.0	32.0	32.0
3	30.765134618555976	32.0	32.0	32.0	32.0	32.0
4	30.844244929444322	32.0	32.0	32.0	32.0	32.0
5	30.737079961899305	32.0	32.0	32.0	32.0	32.0
6	34.3930103064727	36.0	36.0	36.0	32.0	36.0
7	34.31675574551835	36.0	36.0	36.0	32.0	36.0
8	34.28062947252884	36.0	36.0	36.0	32.0	36.0
9	34.446158435095896	36.0	36.0	36.0	32.0	36.0
10	34.119403509625656	36.0	36.0	36.0	32.0	36.0
11	34.4418013663263	36.0	36.0	36.0	32.0	36.0
12	34.22700373347175	36.0	36.0	36.0	32.0	36.0
13	34.32922768590536	36.0	36.0	36.0	32.0	36.0
14	34.20833457241701	36.0	36.0	36.0	32.0	36.0
15	34.1412465271864	36.0	36.0	36.0	32.0	36.0
16	34.1689632519323	36.0	36.0	36.0	32.0	36.0
17	34.08528410161027	36.0	36.0	36.0	32.0	36.0
18	34.10443177425067	36.0	36.0	36.0	32.0	36.0
19	34.104243433532396	36.0	36.0	36.0	32.0	36.0
20	34.09610423081741	36.0	36.0	36.0	32.0	36.0
21	34.063470822057674	36.0	36.0	36.0	32.0	36.0
22	34.05401053085949	36.0	36.0	36.0	32.0	36.0
23	33.992181607312304	36.0	36.0	36.0	32.0	36.0
24	33.95820368012357	36.0	36.0	36.0	32.0	36.0
25	33.950914263520204	36.0	36.0	36.0	32.0	36.0
26	33.902310643233115	36.0	36.0	36.0	32.0	36.0
27	33.92056527447731	36.0	36.0	36.0	32.0	36.0
28	33.893390141940415	36.0	36.0	36.0	32.0	36.0
29	33.85247695079081	36.0	36.0	36.0	32.0	36.0
30	33.825826288579435	36.0	36.0	36.0	32.0	36.0
31	33.85108647362193	36.0	36.0	36.0	32.0	36.0
32	33.79918193443039	36.0	36.0	36.0	32.0	36.0
33	33.77050908766495	36.0	36.0	36.0	32.0	36.0
34	33.77639270751948	36.0	36.0	36.0	32.0	36.0
35	33.72964726216578	36.0	36.0	36.0	27.0	36.0
36	33.68260714018587	36.0	36.0	36.0	27.0	36.0
37	33.68924952982407	36.0	36.0	36.0	27.0	36.0
38	33.069556299906914	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	7.0
10	13.0
11	11.0
12	5.0
13	5.0
14	128.0
15	281.0
16	386.0
17	451.0
18	524.0
19	730.0
20	1075.0
21	1481.0
22	2454.0
23	3884.0
24	5789.0
25	8550.0
26	12620.0
27	18002.0
28	24640.0
29	32921.0
30	43422.0
31	57300.0
32	77152.0
33	112696.0
34	241655.0
35	463506.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.11602220910992	17.22243574822217	11.135140304626416	25.526401738041493
2	16.820817900177083	19.820861179135214	36.79937678300291	26.558944137684794
3	18.575661932632393	24.01658684480431	28.80743073342983	28.600320489133473
4	12.574223252368522	15.350571662105207	36.13875974511089	35.93644534041539
5	14.789859473761046	36.33316091218597	33.5080374087805	15.368942205272482
6	33.9006710006254	35.764711819379535	16.887389766114016	13.447227413881043
7	30.147401393721314	30.769286224723817	20.971513691649296	18.111798689905566
8	28.285337860732024	32.299979814145956	19.75411106545263	19.660571259669386
9	27.637292971273936	14.136573400295038	18.817975126364235	39.4081585020668
10	16.276722577226593	26.039367786830365	31.117727579529035	26.566182056414007
11	37.364767501484685	21.28105559976317	22.37192733289296	18.982249565859185
12	24.942617506819662	24.048559061837913	28.236602415697078	22.772221015645346
13	29.220874615493464	19.064674608103005	25.478239160588316	26.23621161581522
14	23.93047080103499	19.992663871006698	24.821260714668743	31.255604613289567
15	25.404056291614573	26.73139619464647	22.459139185010056	25.405408328728896
16	26.10938219598958	25.642710302020895	23.386047970409866	24.861859531579665
17	24.121132039078226	25.608242516451597	25.145137259603032	25.125488184867145
18	25.240328073617395	24.368595269229626	26.234173764384312	24.156902892768663
19	25.782563124610782	24.8789869420354	25.362408310810018	23.976041622543804
20	25.74498611233776	24.097301954366277	24.905237658365312	25.252474274930652
21	27.373785620302442	23.987310971323517	24.26650565058308	24.372397757790957
22	25.968183243751824	24.034971034711482	24.830840552124904	25.166005169411793
23	24.403019510014566	23.70600152850077	25.55903834229765	26.331940619187012
24	24.879092949266806	25.091344983912105	24.98724685227078	25.042315214550303
25	25.111761249130243	24.145206704232866	25.31311175764393	25.429920288992957
26	24.767484305922967	25.177851184547922	25.65536572945349	24.399298780075622
27	25.454919055906082	24.543413666091983	24.755833895851904	25.24583338215003
28	24.53085079058953	24.448569999504333	25.887086962595134	25.133492247311008
29	24.526331063111094	24.75479526993474	25.720293119126737	24.998580547827427
30	24.58351732528275	24.934168431487404	25.951155769837335	24.531158473392512
31	25.21288685296951	24.690819527643075	24.54554548510445	25.550748134282962
32	24.630161850096478	24.76931021523837	24.56959986734042	26.030928067324737
33	24.353429435329424	24.31557744562156	25.33001076979231	26.000982349256702
34	25.369654364381955	24.41746654048939	25.51710152764634	24.695777567482313
35	26.139157677664976	24.2958114616982	25.25452814951546	24.310502711121366
36	24.683106208089985	25.14312495493547	24.755840363400388	25.417928473574158
37	25.76495091390354	24.876687686613234	24.672826949992835	24.685534449490394
38	24.679010287351918	24.598982529976613	25.491454243138385	25.23055293953308
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	504.0
1	383.0
2	262.0
3	262.0
4	576.0
5	890.0
6	890.0
7	1005.5
8	1121.0
9	1086.5
10	1052.0
11	1052.0
12	1408.0
13	1764.0
14	2253.5
15	2743.0
16	2743.0
17	4021.5
18	5300.0
19	5300.0
20	6303.5
21	7307.0
22	7965.5
23	8624.0
24	8624.0
25	9906.5
26	11189.0
27	11189.0
28	14398.5
29	17608.0
30	20865.5
31	24123.0
32	24123.0
33	30918.0
34	37713.0
35	37713.0
36	41622.5
37	45532.0
38	52115.0
39	58698.0
40	58698.0
41	62340.5
42	65983.0
43	75605.0
44	85227.0
45	85227.0
46	87696.0
47	90165.0
48	90165.0
49	96416.0
50	102667.0
51	103818.5
52	104970.0
53	104970.0
54	99753.5
55	94537.0
56	94537.0
57	91540.5
58	88544.0
59	79680.0
60	70816.0
61	70816.0
62	67824.5
63	64833.0
64	53805.5
65	42778.0
66	42778.0
67	35669.5
68	28561.0
69	28561.0
70	23075.5
71	17590.0
72	13671.5
73	9753.0
74	9753.0
75	7379.0
76	5005.0
77	5005.0
78	5040.0
79	5075.0
80	4002.0
81	2929.0
82	2929.0
83	2916.0
84	2903.0
85	2903.0
86	1940.5
87	978.0
88	849.5
89	721.0
90	721.0
91	519.5
92	318.0
93	272.5
94	227.0
95	227.0
96	204.5
97	182.0
98	182.0
99	340.5
100	499.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11868168706423679
2	0.05469991195747284
3	0.012345779140319244
4	0.002883685638614713
5	4.50575881033549E-4
6	4.50575881033549E-4
7	0.0
8	2.703455286201294E-4
9	1.8023035241341957E-4
10	1.8023035241341957E-4
11	0.002523224933787874
12	0.002883685638614713
13	0.013697506783419888
14	0.010273130087564917
15	0.022889254756504288
16	0.011895203259285694
17	0.020185799470302992
18	0.005587140924816008
19	0.00901151762067098
20	0.0065784078630898146
21	0.006398177510676395
22	0.007840020329983752
23	0.009642323854117948
24	0.014508543369280277
25	0.016491077245827893
26	0.017662574536515117
27	0.008831287268257559
28	0.006848753391709944
29	0.009732439030324658
30	0.0036947222244751014
31	0.005767371277229426
32	0.007569674801363622
33	0.009552208677911237
34	0.012706239845146081
35	0.016851537950654732
36	0.015409695131347373
37	0.009912669382738078
38	0.006848753391709944
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1109691.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.48802072291572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.80890804476503	43.75796568817499
2	11.016111255751714	11.784599742671857
3	3.0515689144975346	4.896671440081487
4	1.2811941855715971	2.741141645917309
5	0.7030570387882744	1.8802564730049494
6	0.4471302220655361	1.4349666350209982
7	0.2876750287415018	1.0771017529153575
8	0.21492108768277016	0.9196562873414079
9	0.16050367200267907	0.7726521360765028
>10	0.8109576044447918	8.073936882929795
>50	0.09589124903601581	3.594804901155106
>100	0.10703284556623327	12.18198688771542
>500	0.011159822153876897	4.014446354669302
>1k	0.0038890289324116467	2.8698131723254927
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3165	0.2852145326942365	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2117	0.19077382802960463	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1572	0.1416610569969478	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1557	0.14030932935384716	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1478	0.13319023043351708	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1469	0.13237919384765667	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1463	0.13183850279041642	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1421	0.12805366538973462	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1370	0.12345779140319244	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1365	0.12300721552215886	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1332	0.12003341470733744	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1315	0.11850145671182337	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1258	0.11336489166804092	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1149	0.10354233746150956	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1126	0.10146968840875524	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1120	0.10092899735151498	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1119	0.10083888217530827	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1118	0.10074876699910154	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.01151762067098E-5
2	0.0	0.0	0.0	0.0	9.01151762067098E-5
3	0.0	0.0	0.0	0.0	9.01151762067098E-5
4	0.0	0.0	0.0	0.0	9.01151762067098E-5
5	0.0	0.0	0.0	0.0	9.01151762067098E-5
6	0.0	0.0	0.0	0.0	1.802303524134196E-4
7	0.0	0.0	0.0	0.0	1.802303524134196E-4
8	0.0	0.0	0.0	0.0	3.604607048268392E-4
9	0.0	0.0	0.0	0.0	3.604607048268392E-4
10	0.0	0.0	0.0	0.0	4.50575881033549E-4
11	0.0	0.0	0.0	0.0	4.50575881033549E-4
12	0.0	0.0	0.0	0.0	0.0010813821144805176
13	0.0	0.0	0.0	0.0	0.0012616124668939372
14	0.0	0.0	0.0	9.01151762067098E-5	0.001351727643100647
15	0.0	0.0	0.0	9.01151762067098E-5	0.0014418428193073568
16	0.0	0.0	0.0	9.01151762067098E-5	0.0014418428193073568
17	0.0	0.0	0.0	1.802303524134196E-4	0.0015319579955140666
18	0.0	0.0	0.0	2.703455286201294E-4	0.0017121883479274862
19	0.0	0.0	0.0	3.604607048268392E-4	0.0017121883479274862
20	0.0	0.0	0.0	5.406910572402588E-4	0.0018924187003409058
21	0.0	0.0	0.0	8.110365858603882E-4	0.0018924187003409058
22	0.0	0.0	0.0	9.912669382738078E-4	0.0018924187003409058
23	0.0	0.0	0.0	0.0014418428193073568	0.0018924187003409058
24	0.0	0.0	0.0	0.0024331097575811646	0.0018924187003409058
25	0.0	0.0	0.0	0.0029738008148214234	0.0018924187003409058
26	0.0	0.0	0.0	0.00432552845792207	0.0019825338765476156
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCCG	35	0.0020679855	22.85431	5
AACCCGT	100	6.816117E-8	17.599405	29
ACCCGTT	100	6.819573E-8	17.598612	30
TAACCCG	110	1.0633812E-8	17.453955	28
CGAATGT	85	4.9852188E-6	16.940605	23
TAACGAA	145	1.2205419E-9	15.448449	13
GATATAT	160	3.0013325E-10	15.012347	1
CAAGACG	405	0.0	15.011161	4
TCTAGCG	355	0.0	14.872738	28
ATAACGA	140	1.2778401E-8	14.85731	12
CATCGTT	260	0.0	14.768731	28
ATACGCG	65	0.0041636415	14.768065	4
CTAATCT	65	0.0041636415	14.768065	4
AAGACGG	390	0.0	14.767401	5
ACCGTCG	295	0.0	14.642253	8
CTAGCGG	365	0.0	14.465263	29
TAGCGGC	365	0.0	14.464612	30
GTATTAG	310	0.0	14.463509	1
GTAGACT	100	2.3272796E-5	14.398865	4
CTAATTG	100	2.3272796E-5	14.398865	30
>>END_MODULE