FastQCFastQC Report
Thu 2 Feb 2017
SRR4062882_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062882_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109691
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33890.3054003321645395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24800.2234856369926403No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16250.14643716133590343No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15180.13679483748178545No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15070.13580357054351166No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14860.13391115184317076No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14840.13373092149075733No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14710.1325594242000701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14360.12940539303283527No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14270.1285943564469749No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA13830.12462928869387965No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13660.12309733069836558No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13190.11886191741665021No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG12430.11201316402494027No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG12420.11192304884873355No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG12170.10967016944356581No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC11930.10750740521460478No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT11190.10083888217530827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCG604.7161193E-621.3355269
TCTAGCG2650.019.92388328
CCTATAC659.432302E-619.6925583
CTAGCGG2800.018.85653329
TAGCGGC2950.017.89772630
ATAGTTA450.00884484917.7796066
GATCTAG550.00136407817.4555511
CTAGTGG753.233177E-517.0668833
CTATACT854.980877E-616.9421564
AGCGGCG3350.016.23828131
AAGACGG4050.015.4089915
CCGTCGT3150.015.2396629
ACCGTCG3050.015.2146798
TAGAACT1901.8189894E-1215.158774
GGTTAAC951.4327428E-515.15740322
CTAGAAC859.430696E-515.0590143
CGAAAAA3100.014.96725724
CGACCCG3000.014.9348695
TATATGC756.2413764E-414.93419554
CGTCGTA3350.014.80682510
Produced by FastQC (version 0.11.5)