##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062882_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1109691
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.196764684943826	32.0	32.0	32.0	32.0	32.0
2	31.29126666792828	32.0	32.0	32.0	32.0	32.0
3	31.376863469199986	32.0	32.0	32.0	32.0	32.0
4	31.466574028265526	32.0	32.0	32.0	32.0	32.0
5	31.41407923466983	32.0	32.0	32.0	32.0	32.0
6	34.91664165970527	36.0	36.0	36.0	36.0	36.0
7	34.944919801998935	36.0	36.0	36.0	36.0	36.0
8	34.87796963298792	36.0	36.0	36.0	32.0	36.0
9	34.99087673956083	36.0	36.0	36.0	36.0	36.0
10	34.83896958702918	36.0	36.0	36.0	32.0	36.0
11	35.00423721558524	36.0	36.0	36.0	36.0	36.0
12	34.90231605014369	36.0	36.0	36.0	32.0	36.0
13	34.94993200809955	36.0	36.0	36.0	36.0	36.0
14	34.89971352385484	36.0	36.0	36.0	32.0	36.0
15	34.86629521191034	36.0	36.0	36.0	32.0	36.0
16	34.880260360767096	36.0	36.0	36.0	32.0	36.0
17	34.85523717863802	36.0	36.0	36.0	32.0	36.0
18	34.8412711286295	36.0	36.0	36.0	32.0	36.0
19	34.83672391683811	36.0	36.0	36.0	32.0	36.0
20	34.82259926411947	36.0	36.0	36.0	32.0	36.0
21	34.815180081662376	36.0	36.0	36.0	32.0	36.0
22	34.803694001303064	36.0	36.0	36.0	32.0	36.0
23	34.73181272985002	36.0	36.0	36.0	32.0	36.0
24	34.71243346120677	36.0	36.0	36.0	32.0	36.0
25	34.700691453747034	36.0	36.0	36.0	32.0	36.0
26	34.64046658033633	36.0	36.0	36.0	32.0	36.0
27	34.62563272118094	36.0	36.0	36.0	32.0	36.0
28	34.59161964907348	36.0	36.0	36.0	32.0	36.0
29	34.55780122574663	36.0	36.0	36.0	32.0	36.0
30	34.53318085845519	36.0	36.0	36.0	32.0	36.0
31	34.526315884331765	36.0	36.0	36.0	32.0	36.0
32	34.48474935815466	36.0	36.0	36.0	32.0	36.0
33	34.43209956645589	36.0	36.0	36.0	32.0	36.0
34	34.426561988877985	36.0	36.0	36.0	32.0	36.0
35	34.38938136832686	36.0	36.0	36.0	32.0	36.0
36	34.361767374881836	36.0	36.0	36.0	32.0	36.0
37	34.36498628897594	36.0	36.0	36.0	32.0	36.0
38	33.93125924243776	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	11.0
21	29.0
22	86.0
23	322.0
24	801.0
25	1892.0
26	3985.0
27	7777.0
28	13506.0
29	21097.0
30	31567.0
31	45557.0
32	64084.0
33	102006.0
34	246469.0
35	570498.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.774553971740055	18.77315530353894	12.022147983060973	26.430142741660035
2	15.895686276630162	20.92699679460318	36.385804696983215	26.791512231783443
3	17.58561617603459	24.72643285383048	29.26922900158693	28.418721968548
4	12.033991475096649	16.20876100532581	36.22606313474934	35.531184384828194
5	14.130986249300479	36.929536113271375	33.25976917857165	15.679708458856489
6	33.819745892768154	36.18617468497931	16.601408142281308	13.392671279971236
7	29.656721165812517	30.90888282308159	21.142562592312565	18.29183341879333
8	28.07821487823759	32.56066492379205	19.629959059568645	19.731161138401728
9	27.476158977399916	13.933232520037993	18.745054711339332	39.845553791222756
10	16.000454184580974	26.24655193699811	31.29755308057	26.455440797850915
11	37.569899236954925	21.13392445970875	22.343062976677874	18.953113326658453
12	24.695229650135893	24.06766586644174	28.28200359019832	22.955100893224042
13	29.202029395596966	19.139939983238563	25.581198352693097	26.07683226847137
14	23.79301985868138	19.88211132648638	24.917206681860087	31.407662132972153
15	25.194310848695718	26.929388451379708	22.336848726357157	25.539451973567417
16	26.021027475216073	25.695531458757436	23.330098198507514	24.953342867518977
17	24.050298686751535	25.807995198663413	25.07698088927458	25.064725225310468
18	25.159346160327516	24.33037665440199	26.26893432496073	24.24134286030976
19	25.71760967692808	24.903599290252874	25.44329908055486	23.93549195226419
20	25.807995198663413	23.994697623031996	24.947935956946573	25.249371221358018
21	27.279510493921727	23.994719245915526	24.30219250421289	24.42357775594986
22	25.768524751484872	24.084362223357672	24.95983116020586	25.187281864951593
23	24.280406492269453	23.604451337266568	25.741897054039462	26.37324511642451
24	24.784467027307603	25.04913529982671	25.06851006271115	25.09788761015454
25	24.947305150713124	24.17979419496058	25.405450706548038	25.467449947778253
26	24.700862403013453	25.11088682424821	25.768998549144357	24.419252223593976
27	25.526070818040008	24.356734184082207	24.8084823765938	25.30871262128398
28	24.550978605756015	24.27423489962521	25.949205679779325	25.22558081483945
29	24.62487305024552	24.599190225026607	25.808175429015822	24.967761295712048
30	24.694351851100894	24.59801872773592	26.085009250322837	24.62262017084035
31	25.16763675653853	24.509886085405757	24.695883809096404	25.626593348959304
32	24.666055685771983	24.6662359161244	24.791856471756553	25.875851926347064
33	24.37696620050086	24.195744581149167	25.510795347533683	25.916493870816293
34	25.241170740323206	24.31100189151755	25.67309277988197	24.774734588277276
35	26.197022414347774	24.196735848087442	25.26324895849385	24.34299277907093
36	24.671192250815768	24.906482975891485	24.96001139055827	25.462313382734475
37	25.80745450760617	24.763560306427646	24.829614730587164	24.59937045537902
38	24.627216044826895	24.417157569089053	25.580093918036646	25.375532468047414
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	386.0
1	287.0
2	188.0
3	188.0
4	502.0
5	816.0
6	816.0
7	1003.5
8	1191.0
9	1121.5
10	1052.0
11	1052.0
12	1384.5
13	1717.0
14	2325.0
15	2933.0
16	2933.0
17	4277.5
18	5622.0
19	5622.0
20	6503.5
21	7385.0
22	8166.0
23	8947.0
24	8947.0
25	10141.5
26	11336.0
27	11336.0
28	14626.0
29	17916.0
30	21176.5
31	24437.0
32	24437.0
33	31517.5
34	38598.0
35	38598.0
36	42160.0
37	45722.0
38	52359.5
39	58997.0
40	58997.0
41	62783.0
42	66569.0
43	76136.5
44	85704.0
45	85704.0
46	88035.0
47	90366.0
48	90366.0
49	96821.0
50	103276.0
51	103564.5
52	103853.0
53	103853.0
54	98959.5
55	94066.0
56	94066.0
57	91525.0
58	88984.0
59	79859.0
60	70734.0
61	70734.0
62	67984.5
63	65235.0
64	53667.5
65	42100.0
66	42100.0
67	35340.5
68	28581.0
69	28581.0
70	22987.0
71	17393.0
72	13554.5
73	9716.0
74	9716.0
75	7263.5
76	4811.0
77	4811.0
78	4809.5
79	4808.0
80	3696.5
81	2585.0
82	2585.0
83	2522.0
84	2459.0
85	2459.0
86	1560.0
87	661.0
88	539.5
89	418.0
90	418.0
91	243.5
92	69.0
93	49.5
94	30.0
95	30.0
96	21.0
97	12.0
98	12.0
99	15.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005767371277229426
2	0.0
3	0.0
4	9.011517620670979E-5
5	1.8023035241341957E-4
6	1.8023035241341957E-4
7	9.01151762067098E-4
8	0.0027034552862012936
9	0.004415643634128779
10	9.01151762067098E-4
11	0.005226680219989168
12	2.703455286201294E-4
13	9.011517620670979E-5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	9.011517620670979E-5
22	0.0
23	1.8023035241341957E-4
24	0.0
25	0.0
26	9.011517620670979E-5
27	1.8023035241341957E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1109691.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.31363834736958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.37986374641739	41.245832586945056
2	11.820905876944632	12.13147378215665
3	3.285704214564543	5.058043133477789
4	1.3924731351904993	2.8581145147037263
5	0.7780483755992819	1.9962246481129966
6	0.48661134772866893	1.4981879227845012
7	0.32132422284684353	1.1541820474388764
8	0.23008206520842397	0.9445078307456778
9	0.17187456635308615	0.7937558405057917
>10	0.9004538442886987	8.48056972447754
>50	0.10216005928792196	3.72311279366523
>100	0.11218296072690512	12.15700223801757
>500	0.013736688632371477	4.680941912856535
>1k	0.004578896210790492	3.278051024112089
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3389	0.3054003321645395	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2480	0.2234856369926403	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1625	0.14643716133590343	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1518	0.13679483748178545	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1507	0.13580357054351166	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1486	0.13391115184317076	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1484	0.13373092149075733	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1471	0.1325594242000701	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1436	0.12940539303283527	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1427	0.1285943564469749	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1383	0.12462928869387965	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1366	0.12309733069836558	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1319	0.11886191741665021	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1243	0.11201316402494027	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1242	0.11192304884873355	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1217	0.10967016944356581	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1193	0.10750740521460478	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1119	0.10083888217530827	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	9.01151762067098E-5	0.0	0.0	0.0	0.0
7	9.01151762067098E-5	0.0	0.0	0.0	0.0
8	9.01151762067098E-5	0.0	0.0	0.0	0.0
9	9.01151762067098E-5	0.0	0.0	0.0	0.0
10	9.01151762067098E-5	0.0	0.0	0.0	0.0
11	9.01151762067098E-5	0.0	0.0	0.0	0.0
12	9.01151762067098E-5	0.0	0.0	0.0	4.50575881033549E-4
13	9.01151762067098E-5	0.0	0.0	0.0	4.50575881033549E-4
14	9.01151762067098E-5	0.0	0.0	9.01151762067098E-5	5.406910572402588E-4
15	9.01151762067098E-5	0.0	0.0	9.01151762067098E-5	7.209214096536784E-4
16	9.01151762067098E-5	0.0	0.0	9.01151762067098E-5	8.110365858603882E-4
17	9.01151762067098E-5	0.0	0.0	1.802303524134196E-4	8.110365858603882E-4
18	9.01151762067098E-5	0.0	0.0	1.802303524134196E-4	9.912669382738078E-4
19	9.01151762067098E-5	0.0	0.0	1.802303524134196E-4	9.912669382738078E-4
20	9.01151762067098E-5	0.0	0.0	5.406910572402588E-4	0.0010813821144805176
21	9.01151762067098E-5	0.0	0.0	8.110365858603882E-4	0.0010813821144805176
22	9.01151762067098E-5	0.0	0.0	9.912669382738078E-4	0.0010813821144805176
23	9.01151762067098E-5	0.0	0.0	0.0015319579955140666	0.0010813821144805176
24	9.01151762067098E-5	0.0	0.0	0.0024331097575811646	0.0010813821144805176
25	9.01151762067098E-5	0.0	0.0	0.0028836856386147136	0.0010813821144805176
26	9.01151762067098E-5	0.0	0.0	0.004235413281715361	0.001351727643100647
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTGCG	60	4.7161193E-6	21.335526	9
TCTAGCG	265	0.0	19.923883	28
CCTATAC	65	9.432302E-6	19.692558	3
CTAGCGG	280	0.0	18.856533	29
TAGCGGC	295	0.0	17.897726	30
ATAGTTA	45	0.008844849	17.779606	6
GATCTAG	55	0.001364078	17.455551	1
CTAGTGG	75	3.233177E-5	17.066883	3
CTATACT	85	4.980877E-6	16.942156	4
AGCGGCG	335	0.0	16.238281	31
AAGACGG	405	0.0	15.408991	5
CCGTCGT	315	0.0	15.239662	9
ACCGTCG	305	0.0	15.214679	8
TAGAACT	190	1.8189894E-12	15.15877	4
GGTTAAC	95	1.4327428E-5	15.157403	22
CTAGAAC	85	9.430696E-5	15.059014	3
CGAAAAA	310	0.0	14.967257	24
CGACCCG	300	0.0	14.934869	5
TATATGC	75	6.2413764E-4	14.9341955	4
CGTCGTA	335	0.0	14.806825	10
>>END_MODULE