FastQCFastQC Report
Thu 2 Feb 2017
SRR4062881_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062881_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929839
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT55150.28577513460967474No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA40140.20799662562524646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34640.1794968388554693No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC32630.1690814622359689No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20300.10519012207754119No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19840.10280650354770528No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19690.10202923663580225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA1401.8189894E-1218.305551
TCTAGCG3950.017.82223728
CTAGCGG4100.017.56088829
CGTTATT2200.017.4589842
TAGCGGC4200.016.76182230
TAGGACG2100.016.7592184
CAAGACG5450.016.7312624
AACGAAT1151.843182E-816.6955731
AAGACCG1001.3180579E-615.9970215
TACGCTG600.00244575115.997025
CGCAAGA5450.015.5635912
ATAACGA1551.891749E-1015.48419712
ACGAACG1551.891749E-1015.48379615
TAACGAA1603.0559022E-1015.00031613
CGAACGA1603.0559022E-1014.99992716
AGCGCGT1308.31933E-814.76915932
TACGCTA3800.014.7371539
GCGCAAG5450.014.6948221
GACGGAC6150.014.568287
AAGACGG6300.014.4734955
Produced by FastQC (version 0.11.5)