##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062881_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1929839
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.224377784882574	32.0	32.0	32.0	32.0	32.0
2	30.779354650828385	32.0	32.0	32.0	32.0	32.0
3	30.8294515760123	32.0	32.0	32.0	32.0	32.0
4	30.90901054440293	32.0	32.0	32.0	32.0	32.0
5	30.800371429948303	32.0	32.0	32.0	32.0	32.0
6	34.46918888052319	36.0	36.0	36.0	32.0	36.0
7	34.386571625923196	36.0	36.0	36.0	32.0	36.0
8	34.35760236993863	36.0	36.0	36.0	32.0	36.0
9	34.51988222851751	36.0	36.0	36.0	32.0	36.0
10	34.20645815531762	36.0	36.0	36.0	32.0	36.0
11	34.524190359921214	36.0	36.0	36.0	32.0	36.0
12	34.320897753646804	36.0	36.0	36.0	32.0	36.0
13	34.42152998255295	36.0	36.0	36.0	32.0	36.0
14	34.30356729240108	36.0	36.0	36.0	32.0	36.0
15	34.24675478109832	36.0	36.0	36.0	32.0	36.0
16	34.259282769184374	36.0	36.0	36.0	32.0	36.0
17	34.19067238251481	36.0	36.0	36.0	32.0	36.0
18	34.21604444723109	36.0	36.0	36.0	32.0	36.0
19	34.20544252655273	36.0	36.0	36.0	32.0	36.0
20	34.19074078200306	36.0	36.0	36.0	32.0	36.0
21	34.17385647196476	36.0	36.0	36.0	32.0	36.0
22	34.137357572315615	36.0	36.0	36.0	32.0	36.0
23	34.074419161391184	36.0	36.0	36.0	32.0	36.0
24	34.06382345884812	36.0	36.0	36.0	32.0	36.0
25	34.03751815565962	36.0	36.0	36.0	32.0	36.0
26	33.98813527967877	36.0	36.0	36.0	32.0	36.0
27	33.991466127485246	36.0	36.0	36.0	32.0	36.0
28	33.97012185990645	36.0	36.0	36.0	32.0	36.0
29	33.92299357614806	36.0	36.0	36.0	32.0	36.0
30	33.895976296468255	36.0	36.0	36.0	32.0	36.0
31	33.91601216474535	36.0	36.0	36.0	32.0	36.0
32	33.86907094322376	36.0	36.0	36.0	32.0	36.0
33	33.829912236202084	36.0	36.0	36.0	32.0	36.0
34	33.827465399963415	36.0	36.0	36.0	32.0	36.0
35	33.77439724246427	36.0	36.0	36.0	32.0	36.0
36	33.731907169458175	36.0	36.0	36.0	27.0	36.0
37	33.71832987104106	36.0	36.0	36.0	27.0	36.0
38	33.109999331550455	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	6.0
8	5.0
9	6.0
10	11.0
11	15.0
12	9.0
13	10.0
14	165.0
15	405.0
16	583.0
17	709.0
18	1001.0
19	1201.0
20	1723.0
21	2612.0
22	3994.0
23	5932.0
24	9052.0
25	13786.0
26	20079.0
27	28552.0
28	40810.0
29	54464.0
30	72899.0
31	97402.0
32	131478.0
33	193943.0
34	418980.0
35	830006.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.25818153142497	16.904901240562964	11.330668758482437	25.50624846952963
2	16.2816030660606	19.662482391253512	37.95452860407035	26.101385938615536
3	19.13028832325787	24.115868273558576	28.4604362032644	28.29340719991916
4	12.434902378181297	15.48154880780021	36.56986186090329	35.513686953115204
5	14.165415263696254	36.98302548302082	34.07313789683465	14.778421356448273
6	33.748654029310295	35.875209992154765	17.184794132552334	13.191341845982606
7	29.962447644596256	30.733807327968808	21.030977195506985	18.27276783192795
8	27.99127384277372	33.17287014918413	19.713290807998632	19.122565200043525
9	27.524638167798116	14.488367116084259	18.600955213176697	39.38603950294093
10	15.413609613711488	26.998896792303146	32.215426564142795	25.372067029842572
11	37.16641690036916	21.517262893019186	22.235470545195263	19.080849661416394
12	24.353595133975787	24.122585422551378	29.193036129856687	22.330783313616152
13	29.55690324613538	19.796730439541534	25.286438952795358	25.359927361527728
14	23.389021727076084	19.862193768247977	25.617904860219333	31.130879644456606
15	24.992225530115185	27.3103133007704	23.062446615306794	24.635014553807626
16	25.437041328798422	26.180295321935944	24.349885962180178	24.032777387085456
17	23.832246755945857	26.000374199319705	25.806536878586833	24.36084216614761
18	24.569915708764608	25.234928264873265	26.81887385748739	23.376282168874738
19	25.119720495848323	25.728881594732457	25.857709709732536	23.293688199686688
20	25.252768129062414	24.500332429408626	26.155380044804126	24.09151939672483
21	26.27244244483898	24.776262181857	24.824144925102893	24.12715044820113
22	25.1002352165687	25.014885648680036	25.49557938986719	24.38929974488408
23	23.783587697250795	24.885238255642214	25.896561552613168	25.43461249449382
24	24.096206339162123	25.75445917491077	25.761973837572384	24.38736064835473
25	24.624877688773903	24.890023550093705	25.66929117534869	24.815807585783702
26	23.9283428307847	25.649286201097194	26.316880586069196	24.10549038204891
27	24.905165230153102	25.469924422388885	25.439141988897596	24.18576835856042
28	23.96937814713227	25.195195397497994	26.191965416977375	24.64346103839236
29	23.867642676685886	25.76764915462849	26.16855606659325	24.196152102092373
30	24.342038102690196	25.578756182683566	26.25038540562809	23.828820308998154
31	24.39280334181639	25.509488638041923	25.349880734548734	24.74782728559295
32	24.0423152542408	25.64634198162829	25.479839934166137	24.831502829964773
33	23.95530876801136	25.24811044289612	25.861267512573292	24.935313276519224
34	24.30449243832135	25.587194845765804	26.12741099441438	23.98090172149847
35	25.125186704658887	25.29346707312396	25.85039372527232	23.73095249694483
36	24.145938992902995	25.898371948634548	25.67137554637205	24.284313512090407
37	25.029292801044743	25.815650984243298	25.298667378365565	23.856388836346394
38	24.11957571112538	25.783545099478005	25.64782613877743	24.44905305061918
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	198.0
1	230.5
2	263.0
3	263.0
4	750.0
5	1237.0
6	1237.0
7	1553.0
8	1869.0
9	1890.5
10	1912.0
11	1912.0
12	2508.0
13	3104.0
14	4058.5
15	5013.0
16	5013.0
17	7366.0
18	9719.0
19	9719.0
20	12039.0
21	14359.0
22	15753.5
23	17148.0
24	17148.0
25	20647.0
26	24146.0
27	24146.0
28	30857.0
29	37568.0
30	45341.0
31	53114.0
32	53114.0
33	64247.5
34	75381.0
35	75381.0
36	83083.0
37	90785.0
38	102217.0
39	113649.0
40	113649.0
41	120746.0
42	127843.0
43	141076.5
44	154310.0
45	154310.0
46	162835.0
47	171360.0
48	171360.0
49	173801.0
50	176242.0
51	173174.0
52	170106.0
53	170106.0
54	160958.0
55	151810.0
56	151810.0
57	145758.5
58	139707.0
59	124680.5
60	109654.0
61	109654.0
62	102859.5
63	96065.0
64	81012.0
65	65959.0
66	65959.0
67	55220.0
68	44481.0
69	44481.0
70	36179.0
71	27877.0
72	22239.5
73	16602.0
74	16602.0
75	12993.5
76	9385.0
77	9385.0
78	8265.0
79	7145.0
80	5611.5
81	4078.0
82	4078.0
83	3833.0
84	3588.0
85	3588.0
86	2488.0
87	1388.0
88	1119.5
89	851.0
90	851.0
91	658.0
92	465.0
93	384.0
94	303.0
95	303.0
96	287.5
97	272.0
98	272.0
99	577.5
100	883.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12058000693322085
2	0.057517751480823005
3	0.012125363825687014
4	0.002331800735709041
5	2.5908897063433786E-4
6	2.5908897063433786E-4
7	0.0
8	4.6636014714180826E-4
9	4.1454235301494063E-4
10	6.21813529522411E-4
11	0.0018136227944403652
12	0.002590889706343379
13	0.014664435737903525
14	0.008964478383948092
15	0.02316255397470981
16	0.011192643531403396
17	0.020001668532970886
18	0.005233597206813626
19	0.007047220001253991
20	0.0054926861774479635
21	0.006477224265858448
22	0.00663267764823905
23	0.009793563089977973
24	0.014405346767269185
25	0.01735896103250064
26	0.019379855003448476
27	0.008653571619186886
28	0.0054926861774479635
29	0.011089007943149662
30	0.002798160882850849
31	0.005699957353955434
32	0.006891766618873387
33	0.007979940295537606
34	0.012488088384575085
35	0.016633511914724494
36	0.0151826136791722
37	0.009534474119343633
38	0.006010864118716639
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1929839.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.93925931951217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.37291830207513	41.19498645283547
2	13.982330220342515	15.083930712922132
3	4.6070684870211345	7.455055854725567
4	1.9261117672883257	4.155721683765155
5	0.9542736351825752	2.5736406534943237
6	0.5391630947804772	1.7449234790924928
7	0.3269919047953798	1.234639080369746
8	0.224427156932007	0.9684347692877102
9	0.15644165238320057	0.7594512170643392
>10	0.7502213626357912	7.339459597001616
>50	0.07408347397572829	2.792361369104129
>100	0.0734269929660385	8.24297957174229
>500	0.00916527087739036	3.3161679859992
>1k	0.0032802022087502335	2.851253983850642
>5k	9.647653555147746E-5	0.2869935887451642
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5515	0.28577513460967474	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	4014	0.20799662562524646	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3464	0.1794968388554693	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3263	0.1690814622359689	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2030	0.10519012207754119	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1984	0.10280650354770528	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1969	0.10202923663580225	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.181779412686758E-5	0.0	0.0	0.0
6	0.0	5.181779412686758E-5	0.0	0.0	0.0
7	0.0	5.181779412686758E-5	0.0	0.0	0.0
8	0.0	5.181779412686758E-5	0.0	0.0	0.0
9	0.0	5.181779412686758E-5	0.0	5.181779412686758E-5	0.0
10	0.0	5.181779412686758E-5	0.0	4.1454235301494063E-4	5.181779412686758E-5
11	0.0	5.181779412686758E-5	0.0	4.1454235301494063E-4	5.181779412686758E-5
12	0.0	5.181779412686758E-5	0.0	4.663601471418082E-4	1.0363558825373516E-4
13	0.0	5.181779412686758E-5	0.0	5.181779412686758E-4	1.0363558825373516E-4
14	0.0	5.181779412686758E-5	0.0	5.181779412686758E-4	1.5545338238060274E-4
15	0.0	5.181779412686758E-5	0.0	6.21813529522411E-4	1.5545338238060274E-4
16	0.0	5.181779412686758E-5	0.0	6.21813529522411E-4	1.5545338238060274E-4
17	0.0	5.181779412686758E-5	0.0	6.21813529522411E-4	1.5545338238060274E-4
18	0.0	5.181779412686758E-5	0.0	7.254491177761461E-4	1.5545338238060274E-4
19	0.0	5.181779412686758E-5	0.0	7.772669119030137E-4	1.5545338238060274E-4
20	0.0	5.181779412686758E-5	0.0	8.809025001567488E-4	1.5545338238060274E-4
21	0.0	5.181779412686758E-5	0.0	8.809025001567488E-4	2.590889706343379E-4
22	0.0	5.181779412686758E-5	0.0	0.0010363558825373517	2.590889706343379E-4
23	0.0	5.181779412686758E-5	0.0	0.0015545338238060274	2.590889706343379E-4
24	0.0	5.181779412686758E-5	0.0	0.002331800735709041	2.590889706343379E-4
25	0.0	5.181779412686758E-5	0.0	0.0029017964711045845	2.590889706343379E-4
26	0.0	5.181779412686758E-5	0.0	0.0040417879418956715	2.590889706343379E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAA	140	1.8189894E-12	18.30555	1
TCTAGCG	395	0.0	17.822237	28
CTAGCGG	410	0.0	17.560888	29
CGTTATT	220	0.0	17.458984	2
TAGCGGC	420	0.0	16.761822	30
TAGGACG	210	0.0	16.759218	4
CAAGACG	545	0.0	16.731262	4
AACGAAT	115	1.843182E-8	16.69557	31
AAGACCG	100	1.3180579E-6	15.997021	5
TACGCTG	60	0.002445751	15.99702	5
CGCAAGA	545	0.0	15.563591	2
ATAACGA	155	1.891749E-10	15.484197	12
ACGAACG	155	1.891749E-10	15.483796	15
TAACGAA	160	3.0559022E-10	15.000316	13
CGAACGA	160	3.0559022E-10	14.999927	16
AGCGCGT	130	8.31933E-8	14.769159	32
TACGCTA	380	0.0	14.737153	9
GCGCAAG	545	0.0	14.694822	1
GACGGAC	615	0.0	14.56828	7
AAGACGG	630	0.0	14.473495	5
>>END_MODULE