FastQCFastQC Report
Thu 2 Feb 2017
SRR4062881_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062881_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929839
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT61620.319301247409758No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT43830.2271173916580606No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA40210.20835935018413454No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC38690.20048304547685067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT25820.13379354443557206No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20370.10555284663642925No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20050.1038946772243695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAA350.002067434622.85640312
CGCGCCA1950.017.23065810
GTATTAG3900.016.4122751
CGCAAGA5100.015.9998972
ATAACGA1800.015.99948312
CTAGCGG4150.015.80671629
CAAGACG5500.015.7098044
CGGTCCA4400.015.63626310
ACGAACG1850.015.56746715
GAGCGTT1753.6379788E-1215.5439657
CATCGTT3000.015.466166528
CCGATAA2000.015.200699
CGATAAC2000.015.19990310
CGAACGA1901.8189894E-1215.15779616
TCTAGCG4350.015.0799728
ATACCGT3400.015.0598966
GCGTTCC1704.7293724E-1115.0595069
TATACCG756.243698E-414.9343985
CGTCGTA2900.014.89645610
CGTTTAT3550.014.87275831
Produced by FastQC (version 0.11.5)