##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062881_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1929839
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1883229637291	32.0	32.0	32.0	32.0	32.0
2	31.29115226710622	32.0	32.0	32.0	32.0	32.0
3	31.381541154469364	32.0	32.0	32.0	32.0	32.0
4	31.474223497400562	32.0	32.0	32.0	32.0	32.0
5	31.413715859198618	32.0	32.0	32.0	32.0	32.0
6	34.92939721914626	36.0	36.0	36.0	36.0	36.0
7	34.95968109256783	36.0	36.0	36.0	36.0	36.0
8	34.88834042632572	36.0	36.0	36.0	32.0	36.0
9	35.016164042699934	36.0	36.0	36.0	36.0	36.0
10	34.85557758963313	36.0	36.0	36.0	32.0	36.0
11	35.025917706088435	36.0	36.0	36.0	36.0	36.0
12	34.92940188274773	36.0	36.0	36.0	32.0	36.0
13	34.98002009494056	36.0	36.0	36.0	36.0	36.0
14	34.91638628921895	36.0	36.0	36.0	32.0	36.0
15	34.88242646148202	36.0	36.0	36.0	32.0	36.0
16	34.90283282698712	36.0	36.0	36.0	32.0	36.0
17	34.87700113843694	36.0	36.0	36.0	32.0	36.0
18	34.86895694407668	36.0	36.0	36.0	32.0	36.0
19	34.86283156263295	36.0	36.0	36.0	32.0	36.0
20	34.853898174925476	36.0	36.0	36.0	32.0	36.0
21	34.83775745023289	36.0	36.0	36.0	32.0	36.0
22	34.82195250484626	36.0	36.0	36.0	32.0	36.0
23	34.748405436930234	36.0	36.0	36.0	32.0	36.0
24	34.739975200003734	36.0	36.0	36.0	32.0	36.0
25	34.71427253776092	36.0	36.0	36.0	32.0	36.0
26	34.652112948282216	36.0	36.0	36.0	32.0	36.0
27	34.639436243127015	36.0	36.0	36.0	32.0	36.0
28	34.60623710060788	36.0	36.0	36.0	32.0	36.0
29	34.56235312893977	36.0	36.0	36.0	32.0	36.0
30	34.55171700851729	36.0	36.0	36.0	32.0	36.0
31	34.52989808994429	36.0	36.0	36.0	32.0	36.0
32	34.49255766931853	36.0	36.0	36.0	32.0	36.0
33	34.44899289526225	36.0	36.0	36.0	32.0	36.0
34	34.432749053159355	36.0	36.0	36.0	32.0	36.0
35	34.384383878655164	36.0	36.0	36.0	32.0	36.0
36	34.35864649849029	36.0	36.0	36.0	32.0	36.0
37	34.35061733129033	36.0	36.0	36.0	32.0	36.0
38	33.92773438613273	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	10.0
21	35.0
22	133.0
23	468.0
24	1311.0
25	3173.0
26	6591.0
27	13253.0
28	22796.0
29	36009.0
30	54254.0
31	77929.0
32	111554.0
33	176871.0
34	431575.0
35	993876.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.03160580760407	18.321149083499467	12.149079871916388	26.498165236980075
2	15.422426430391342	20.775878195020415	37.2830583276636	26.518637046924642
3	18.054614918653836	24.823832454417182	28.828726126894523	28.292826500034458
4	11.933501085066297	16.336776803832034	36.44895213893823	35.28076997216344
5	13.62000333706767	37.51185332654865	33.80915911076474	15.058984225618937
6	33.729481395809174	36.355665824107156	16.731594530928568	13.183258249155108
7	29.643507274478726	30.92771432080252	21.015229846789534	18.413548557929225
8	27.862038660225195	33.49006911125424	19.48748747918812	19.16040474933244
9	27.347729184166603	14.294301545936328	18.42971879017644	39.92825047972063
10	15.178482597376755	27.22232701013149	32.385699421294376	25.213490971197373
11	37.239078490600065	21.4637981041038	22.07356715673091	19.223556248565227
12	24.038906973345895	24.311262004918557	29.228472500743585	22.42135852099196
13	29.528291034358396	19.806263129367604	25.31171074817834	25.353735088095657
14	23.233699806045998	19.78040655204916	25.676027896627645	31.3098657452772
15	25.01447011900993	27.359639845603702	22.92543574878526	24.700454286601108
16	25.382454580360932	26.16625134661632	24.260598174871763	24.190695898150985
17	23.66404658626963	26.1014001686151	25.84220756239251	24.392345682722755
18	24.55754081039921	25.15328998947581	26.880066160959544	23.409103039165444
19	25.07846509475661	25.674680633980344	25.882418170634956	23.364436100628083
20	25.217336783016613	24.51173388039106	26.09844655434987	24.172482782242458
21	26.197082966074337	24.77457940748364	24.87396603961889	24.154371586823135
22	24.9284007629652	24.984882158563487	25.686495091041273	24.40022198743004
23	23.653344816168413	24.778362110375124	26.01991774434836	25.548375329108108
24	24.011070353537264	25.693956853395544	25.856871998130416	24.43810079493678
25	24.536774589369678	24.825192580931663	25.77475415034837	24.863278679350287
26	23.951336847963404	25.554994771581864	26.26691981399475	24.22674856645998
27	24.88109111692737	25.351855776569966	25.512750027333887	24.25430307916878
28	23.916710150432237	25.123442940058734	26.338881119098534	24.620965790410494
29	23.91339381160812	25.586953108523563	26.327792111155386	24.171860968712934
30	24.28409831079173	25.3806664701045	26.377692646899558	23.95754257220421
31	24.32757344006417	25.323563260976695	25.507257341156436	24.8416059578027
32	24.11154505634926	25.485649321005532	25.590217629553553	24.81258799309165
33	23.868415966305996	25.076599654168042	26.053313255665365	25.001671123860593
34	24.222486953574883	25.40424356643223	26.38593167616573	23.98733780382716
35	25.15836813330024	25.155777243593896	25.90013985622635	23.78571476687952
36	24.138749398265865	25.71116036104566	25.761112714583962	24.38897752610451
37	25.060587955782843	25.607110230438913	25.376624682162607	23.955677131615644
38	24.128126750469857	25.457823165559407	25.856612909159782	24.557437174810957
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	108.0
1	125.0
2	142.0
3	142.0
4	639.5
5	1137.0
6	1137.0
7	1485.5
8	1834.0
9	1930.5
10	2027.0
11	2027.0
12	2601.5
13	3176.0
14	4211.5
15	5247.0
16	5247.0
17	7755.0
18	10263.0
19	10263.0
20	12442.5
21	14622.0
22	16111.0
23	17600.0
24	17600.0
25	20847.5
26	24095.0
27	24095.0
28	30962.5
29	37830.0
30	45397.5
31	52965.0
32	52965.0
33	64682.0
34	76399.0
35	76399.0
36	83587.5
37	90776.0
38	101942.0
39	113108.0
40	113108.0
41	120256.0
42	127404.0
43	140793.0
44	154182.0
45	154182.0
46	163597.0
47	173012.0
48	173012.0
49	175339.5
50	177667.0
51	173999.0
52	170331.0
53	170331.0
54	161231.0
55	152131.0
56	152131.0
57	146046.5
58	139962.0
59	124703.5
60	109445.0
61	109445.0
62	102825.5
63	96206.0
64	80825.5
65	65445.0
66	65445.0
67	55075.5
68	44706.0
69	44706.0
70	36122.5
71	27539.0
72	21848.0
73	16157.0
74	16157.0
75	12525.5
76	8894.0
77	8894.0
78	7904.0
79	6914.0
80	5304.0
81	3694.0
82	3694.0
83	3411.0
84	3128.0
85	3128.0
86	2039.5
87	951.0
88	742.5
89	534.0
90	534.0
91	330.0
92	126.0
93	83.0
94	40.0
95	40.0
96	30.0
97	20.0
98	20.0
99	21.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008394482648552548
2	0.0
3	0.0
4	1.5545338238060274E-4
5	5.181779412686758E-5
6	2.5908897063433786E-4
7	5.181779412686757E-4
8	0.002694525294597114
9	0.0036272455888807305
10	7.772669119030136E-4
11	0.004767237059671817
12	2.5908897063433786E-4
13	2.5908897063433786E-4
14	0.0
15	0.0
16	1.0363558825373516E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0363558825373516E-4
22	0.0
23	1.0363558825373516E-4
24	0.0
25	5.181779412686758E-5
26	5.181779412686758E-5
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1929839.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.6994040830726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.3442282346725	38.435522967485085
2	15.149955686123892	15.664873617151246
3	5.038167394859743	7.8141075595504494
4	2.0748147269160966	4.290667398573808
5	1.0647139676850594	2.7522538824120693
6	0.5724588413856033	1.7757468577033122
7	0.36045183746689824	1.3044601638381024
8	0.23193779716037663	0.959283671802563
9	0.17784464089814173	0.827501575842177
>10	0.8147799747490737	7.666784888267575
>50	0.07756652313595992	2.821055217472084
>100	0.0784749750143115	8.411534177482034
>500	0.010777777881061124	3.772640303397423
>1k	0.003726895155133286	3.1826803056447606
>5k	1.0072689608468341E-4	0.32088741337734206
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6162	0.319301247409758	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4383	0.2271173916580606	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	4021	0.20835935018413454	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3869	0.20048304547685067	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2582	0.13379354443557206	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2037	0.10555284663642925	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2005	0.1038946772243695	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.181779412686758E-5
2	0.0	0.0	0.0	0.0	5.181779412686758E-5
3	0.0	0.0	0.0	5.181779412686758E-5	5.181779412686758E-5
4	0.0	0.0	0.0	1.5545338238060274E-4	5.181779412686758E-5
5	0.0	5.181779412686758E-5	0.0	2.0727117650747032E-4	5.181779412686758E-5
6	0.0	5.181779412686758E-5	0.0	2.0727117650747032E-4	5.181779412686758E-5
7	0.0	5.181779412686758E-5	0.0	2.0727117650747032E-4	5.181779412686758E-5
8	0.0	5.181779412686758E-5	0.0	2.0727117650747032E-4	5.181779412686758E-5
9	0.0	5.181779412686758E-5	0.0	2.0727117650747032E-4	5.181779412686758E-5
10	0.0	5.181779412686758E-5	0.0	5.699957353955434E-4	5.181779412686758E-5
11	0.0	5.181779412686758E-5	0.0	5.699957353955434E-4	5.181779412686758E-5
12	0.0	5.181779412686758E-5	0.0	6.21813529522411E-4	2.590889706343379E-4
13	0.0	5.181779412686758E-5	0.0	6.21813529522411E-4	2.590889706343379E-4
14	0.0	5.181779412686758E-5	0.0	6.21813529522411E-4	2.590889706343379E-4
15	0.0	5.181779412686758E-5	0.0	6.736313236492785E-4	2.590889706343379E-4
16	0.0	5.181779412686758E-5	0.0	6.736313236492785E-4	2.590889706343379E-4
17	0.0	5.181779412686758E-5	0.0	6.736313236492785E-4	2.590889706343379E-4
18	0.0	5.181779412686758E-5	0.0	7.254491177761461E-4	2.590889706343379E-4
19	0.0	5.181779412686758E-5	0.0	7.254491177761461E-4	2.590889706343379E-4
20	0.0	5.181779412686758E-5	0.0	7.254491177761461E-4	2.590889706343379E-4
21	0.0	5.181779412686758E-5	0.0	8.290847060298813E-4	4.1454235301494063E-4
22	0.0	5.181779412686758E-5	0.0	9.327202942836164E-4	4.1454235301494063E-4
23	0.0	5.181779412686758E-5	0.0	0.0014508982355522922	5.181779412686758E-4
24	0.0	5.181779412686758E-5	0.0	0.002124529559201571	5.181779412686758E-4
25	0.0	5.181779412686758E-5	0.0	0.0026427075004702466	5.181779412686758E-4
26	0.0	5.181779412686758E-5	0.0	0.0036272455888807305	5.181779412686758E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCAA	35	0.0020674346	22.856403	12
CGCGCCA	195	0.0	17.230658	10
GTATTAG	390	0.0	16.412275	1
CGCAAGA	510	0.0	15.999897	2
ATAACGA	180	0.0	15.999483	12
CTAGCGG	415	0.0	15.806716	29
CAAGACG	550	0.0	15.709804	4
CGGTCCA	440	0.0	15.636263	10
ACGAACG	185	0.0	15.567467	15
GAGCGTT	175	3.6379788E-12	15.543965	7
CATCGTT	300	0.0	15.4661665	28
CCGATAA	200	0.0	15.20069	9
CGATAAC	200	0.0	15.199903	10
CGAACGA	190	1.8189894E-12	15.157796	16
TCTAGCG	435	0.0	15.07997	28
ATACCGT	340	0.0	15.059896	6
GCGTTCC	170	4.7293724E-11	15.059506	9
TATACCG	75	6.243698E-4	14.934398	5
CGTCGTA	290	0.0	14.896456	10
CGTTTAT	355	0.0	14.872758	31
>>END_MODULE