FastQCFastQC Report
Thu 2 Feb 2017
SRR4062880_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062880_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83681
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3280.39196472317491426No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1830.21868763518600398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT1380.16491198718944564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT840.1003812095935756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA200.003730712532.00538617
CTGTACC252.889691E-431.9671274
ATAGCAT308.3724404E-426.6552056
GTATCAA1100.026.1705631
TACTTTT703.6407512E-416.00269315
TCAACGC1800.015.9835624
CAACGCA1850.015.5608765
ATCAACG1901.8189894E-1215.1423233
GAGTACT756.1510876E-414.93584712
AACGCAG1951.8189894E-1214.7628816
ACGCAGA2053.6379788E-1214.0511467
CAGAGTA2053.6379788E-1214.05114610
GTACTTT800.001002861914.00235714
ACTTTTT800.001002861914.00235716
AGAGTAC2105.456968E-1213.71659311
TATCAAC2105.456968E-1213.7001962
AGTACTT850.001584661913.17868813
GCAGAGT2201.4551915E-1113.0931129
CGCAGAG2459.458745E-1111.757088
AGTACAT1559.247016E-611.3567513
Produced by FastQC (version 0.11.5)