##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062880_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	83681
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35814581565708	32.0	32.0	32.0	32.0	32.0
2	31.03357990463785	32.0	32.0	32.0	32.0	32.0
3	31.04827858175691	32.0	32.0	32.0	32.0	32.0
4	31.134439119991395	32.0	32.0	32.0	32.0	32.0
5	31.084798221818573	32.0	32.0	32.0	32.0	32.0
6	34.74640599419223	36.0	36.0	36.0	32.0	36.0
7	34.68981011221185	36.0	36.0	36.0	32.0	36.0
8	34.7046999916349	36.0	36.0	36.0	32.0	36.0
9	34.807100775564344	36.0	36.0	36.0	32.0	36.0
10	34.5906119668742	36.0	36.0	36.0	32.0	36.0
11	34.7625147883032	36.0	36.0	36.0	32.0	36.0
12	34.63706217659923	36.0	36.0	36.0	32.0	36.0
13	34.645510928406686	36.0	36.0	36.0	32.0	36.0
14	34.64588138287066	36.0	36.0	36.0	32.0	36.0
15	34.57735925717905	36.0	36.0	36.0	32.0	36.0
16	34.588365339802344	36.0	36.0	36.0	32.0	36.0
17	34.582115414490744	36.0	36.0	36.0	32.0	36.0
18	34.55188155017268	36.0	36.0	36.0	32.0	36.0
19	34.54412590671718	36.0	36.0	36.0	32.0	36.0
20	34.54946762108484	36.0	36.0	36.0	32.0	36.0
21	34.53512744828575	36.0	36.0	36.0	32.0	36.0
22	34.52750325641424	36.0	36.0	36.0	32.0	36.0
23	34.45400987081894	36.0	36.0	36.0	32.0	36.0
24	34.45085503280315	36.0	36.0	36.0	32.0	36.0
25	34.41847014256522	36.0	36.0	36.0	32.0	36.0
26	34.40880247606984	36.0	36.0	36.0	32.0	36.0
27	34.430420286564456	36.0	36.0	36.0	32.0	36.0
28	34.390889210214986	36.0	36.0	36.0	32.0	36.0
29	34.39163011914294	36.0	36.0	36.0	32.0	36.0
30	34.319355648235565	36.0	36.0	36.0	32.0	36.0
31	34.33491473572256	36.0	36.0	36.0	32.0	36.0
32	34.31617690993176	36.0	36.0	36.0	32.0	36.0
33	34.31291452061998	36.0	36.0	36.0	32.0	36.0
34	34.336432404010466	36.0	36.0	36.0	32.0	36.0
35	34.31990535485953	36.0	36.0	36.0	32.0	36.0
36	34.240699800432594	36.0	36.0	36.0	32.0	36.0
37	34.20979672805057	36.0	36.0	36.0	32.0	36.0
38	33.71772564859407	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	2.0
14	4.0
15	18.0
16	21.0
17	31.0
18	27.0
19	38.0
20	48.0
21	78.0
22	121.0
23	177.0
24	243.0
25	385.0
26	629.0
27	934.0
28	1306.0
29	1823.0
30	2554.0
31	3395.0
32	4648.0
33	7129.0
34	17117.0
35	42952.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1125095712098	18.391079632465544	12.33489663093415	25.161514165390503
2	16.648132869390537	20.26162547380756	38.25256184907511	24.8376798077268
3	19.519279038079983	23.939234575574307	29.277125714149115	27.26436067219659
4	12.895708600724195	16.47127714241327	36.565924544987396	34.06708971187514
5	14.750239005736137	36.06477055449331	34.61161567877629	14.573374760994264
6	33.046928215484996	36.08584983449051	17.351609086889496	13.515612863135
7	28.883498046151455	30.98074831801723	21.57000991861952	18.56574371721179
8	27.107706647865104	34.296913277805	20.09297212031405	18.502407954015844
9	26.638065988695164	14.60188095266548	19.420179013157107	39.33987404548225
10	15.697709157395344	26.93681958867604	34.22879745700936	23.13667379691925
11	36.21099678533443	22.38673980329593	22.319817397435436	19.082446013934202
12	22.588432122370936	24.067877629063098	30.546128107074573	22.797562141491394
13	29.903302535170862	20.73676535625067	25.471235791209974	23.888696317368492
14	22.803977340631498	19.075220498601716	26.84824437698688	31.27255778377991
15	25.058573204551976	26.70579516113608	23.606196805967294	24.62943482834465
16	24.83507027440482	26.607467253083467	24.498039965579885	24.05942250693183
17	23.06359072436051	26.519244561319628	26.1953143676787	24.221850346641165
18	23.943544146469716	26.92647832114346	25.816243606290932	23.313733926095896
19	24.77532386825374	25.6489315932884	26.22615803814714	23.349586500310725
20	25.039737077980277	25.694651927098892	26.381834478637582	22.883776516283238
21	26.146969896148285	25.15386545884771	25.456218554680497	23.242946090323503
22	25.121003884075293	25.030176277263223	25.544069315805203	24.30475052285629
23	24.94113991371171	26.184073715535476	25.47058190814241	23.4042044626104
24	23.69962231677583	26.21910407802266	25.718315245972175	24.362958359229335
25	24.575389948006933	25.32480726707703	26.116058088806547	23.983744696109486
26	23.931675014045112	25.880060722695703	26.406005331165805	23.782258932093377
27	24.305040992422974	25.881396849678513	25.997322943805724	23.81623921409279
28	24.438920624297904	25.34357895743206	26.127536508998784	24.089963909271255
29	23.96495673375723	26.216713677869674	25.859348854998327	23.958980733374766
30	24.361241903487176	25.81801668299912	24.955185353378425	24.86555606013528
31	24.184662452047757	25.720329361712295	25.97129438196876	24.123713804271183
32	23.50705689735531	26.24616083272584	26.52461249805801	23.722169771860845
33	24.02213272464357	26.26528197712633	25.845811871840528	23.86677342638957
34	25.253973945261144	24.766343970359745	26.107326401338593	23.872355683040517
35	24.41583898072119	26.04611137007422	25.991131509436215	23.546918139768366
36	24.55238687160854	25.629287882771973	26.03207993689193	23.786245308727562
37	24.012524948310684	25.302068767702846	26.394416358921035	24.290989925065436
38	24.292799521959964	25.125784284433824	26.10337615775321	24.478040035853002
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	4.0
2	8.0
3	8.0
4	17.0
5	26.0
6	26.0
7	32.5
8	39.0
9	31.0
10	23.0
11	23.0
12	43.0
13	63.0
14	114.0
15	165.0
16	165.0
17	285.0
18	405.0
19	405.0
20	452.0
21	499.0
22	612.0
23	725.0
24	725.0
25	976.5
26	1228.0
27	1228.0
28	1653.5
29	2079.0
30	2630.5
31	3182.0
32	3182.0
33	3676.5
34	4171.0
35	4171.0
36	4527.0
37	4883.0
38	5126.5
39	5370.0
40	5370.0
41	5936.0
42	6502.0
43	6589.5
44	6677.0
45	6677.0
46	7045.0
47	7413.0
48	7413.0
49	7463.0
50	7513.0
51	6998.0
52	6483.0
53	6483.0
54	6227.5
55	5972.0
56	5972.0
57	5411.5
58	4851.0
59	4227.5
60	3604.0
61	3604.0
62	3114.0
63	2624.0
64	2428.5
65	2233.0
66	2233.0
67	2000.5
68	1768.0
69	1768.0
70	1623.0
71	1478.0
72	1342.0
73	1206.0
74	1206.0
75	1098.5
76	991.0
77	991.0
78	838.5
79	686.0
80	493.5
81	301.0
82	301.0
83	201.0
84	101.0
85	101.0
86	61.0
87	21.0
88	21.5
89	22.0
90	22.0
91	14.5
92	7.0
93	7.0
94	7.0
95	7.0
96	10.0
97	13.0
98	13.0
99	177.5
100	342.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11591639679258134
2	0.059750719996175955
3	0.017925215998852786
4	0.002390028799847038
5	0.001195014399923519
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.002390028799847038
12	0.001195014399923519
13	0.021510259198623344
14	0.008365100799464634
15	0.029875359998087977
16	0.010755129599311672
17	0.0250953023983939
18	0.0059750719996175955
19	0.0059750719996175955
20	0.007170086399541114
21	0.004780057599694076
22	0.007170086399541114
23	0.009560115199388153
24	0.015535187199005747
25	0.019120230398776306
26	0.026290316798317417
27	0.008365100799464634
28	0.003585043199770557
29	0.015535187199005747
30	0.003585043199770557
31	0.004780057599694076
32	0.004780057599694076
33	0.004780057599694076
34	0.013145158399158708
35	0.016730201598929267
36	0.017925215998852786
37	0.009560115199388153
38	0.007170086399541114
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	83681.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.167791971893266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.4146936783012	22.879745700935697
2	15.404564315352697	12.067255410427697
3	7.947888699048083	9.339037535402301
4	4.954845008542836	7.76281354190318
5	3.133390285574811	6.13639894360727
6	2.324871857456676	5.463605836450329
7	1.6444959726629242	4.508789330911438
8	1.1898950451549914	3.72844492776138
9	1.0495484500854282	3.6997645821632155
>10	3.920551623138882	23.476057886497532
>50	0.0061020258725897	0.1625219583895986
>100	0.00915303880888455	0.7755643455503639
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	328	0.39196472317491426	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	183	0.21868763518600398	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	138	0.16491198718944564	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	84	0.1003812095935756	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.001195014399923519	0.0
18	0.0	0.0	0.0	0.001195014399923519	0.0
19	0.0	0.0	0.0	0.001195014399923519	0.0
20	0.0	0.0	0.0	0.001195014399923519	0.0
21	0.0	0.0	0.0	0.001195014399923519	0.0
22	0.0	0.0	0.0	0.002390028799847038	0.0
23	0.0	0.0	0.0	0.002390028799847038	0.0
24	0.0	0.0	0.0	0.0035850431997705573	0.0
25	0.0	0.0	0.0	0.0035850431997705573	0.0
26	0.0	0.0	0.0	0.0035850431997705573	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGGA	20	0.0037307125	32.005386	17
CTGTACC	25	2.889691E-4	31.967127	4
ATAGCAT	30	8.3724404E-4	26.655205	6
GTATCAA	110	0.0	26.170563	1
TACTTTT	70	3.6407512E-4	16.002693	15
TCAACGC	180	0.0	15.983562	4
CAACGCA	185	0.0	15.560876	5
ATCAACG	190	1.8189894E-12	15.142323	3
GAGTACT	75	6.1510876E-4	14.935847	12
AACGCAG	195	1.8189894E-12	14.762881	6
ACGCAGA	205	3.6379788E-12	14.051146	7
CAGAGTA	205	3.6379788E-12	14.051146	10
GTACTTT	80	0.0010028619	14.002357	14
ACTTTTT	80	0.0010028619	14.002357	16
AGAGTAC	210	5.456968E-12	13.716593	11
TATCAAC	210	5.456968E-12	13.700196	2
AGTACTT	85	0.0015846619	13.178688	13
GCAGAGT	220	1.4551915E-11	13.093112	9
CGCAGAG	245	9.458745E-11	11.75708	8
AGTACAT	155	9.247016E-6	11.35675	13
>>END_MODULE