FastQCFastQC Report
Thu 2 Feb 2017
SRR4062880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83681
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1940.23183279358516268No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT1160.13862167039112822No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACAT1150.13742665599120468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAC200.003725271232.014953
ACACTCC200.003725271232.014956
TTATACA200.00373620631.9958152
CTTATAC200.00373620631.9958151
GGTATCA200.00373620631.9958151
TCGTAGA200.00373620631.9958152
TATACAC356.34053E-527.441393
GTATCAA701.0841177E-925.139571
TAAGACC400.00443738220.0093444
ATACACA400.00443738220.0093444
CTCTTAG400.004452785519.9973851
TCTAAGA400.004452785519.9973852
ATGGGGG450.00876012817.7860835
CTGTCTC450.00876012817.7860839
TAGGGAT450.00876012817.7860834
CAACGCA1205.216734E-714.673525
TCAACGC1205.216734E-714.673524
TACACAT700.00671376813.7206955
ATCAACG1453.017667E-713.2475673
GTACATG1903.781679E-912.6299271
Produced by FastQC (version 0.11.5)