##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062880_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	83681
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28388762084583	32.0	32.0	32.0	32.0	32.0
2	31.40343686141418	32.0	32.0	32.0	32.0	32.0
3	31.45047262819517	32.0	32.0	32.0	32.0	32.0
4	31.52371506076648	32.0	32.0	32.0	32.0	32.0
5	31.486478412064866	32.0	32.0	32.0	32.0	32.0
6	35.00554486681565	36.0	36.0	36.0	36.0	36.0
7	35.02259772230256	36.0	36.0	36.0	36.0	36.0
8	34.942161303043704	36.0	36.0	36.0	36.0	36.0
9	35.07284807781934	36.0	36.0	36.0	36.0	36.0
10	34.925371350724774	36.0	36.0	36.0	32.0	36.0
11	35.07098385535546	36.0	36.0	36.0	36.0	36.0
12	35.00244977951984	36.0	36.0	36.0	36.0	36.0
13	35.0249758009584	36.0	36.0	36.0	36.0	36.0
14	34.97411598809766	36.0	36.0	36.0	36.0	36.0
15	34.961795389634446	36.0	36.0	36.0	36.0	36.0
16	34.97914699872133	36.0	36.0	36.0	36.0	36.0
17	34.97476129587361	36.0	36.0	36.0	36.0	36.0
18	34.95249817760304	36.0	36.0	36.0	36.0	36.0
19	34.93791900192397	36.0	36.0	36.0	36.0	36.0
20	34.94257955808367	36.0	36.0	36.0	36.0	36.0
21	34.949307489155245	36.0	36.0	36.0	36.0	36.0
22	34.921810207813	36.0	36.0	36.0	36.0	36.0
23	34.85865369677705	36.0	36.0	36.0	32.0	36.0
24	34.853491234569375	36.0	36.0	36.0	32.0	36.0
25	34.86117517716089	36.0	36.0	36.0	32.0	36.0
26	34.80189051278068	36.0	36.0	36.0	32.0	36.0
27	34.76348274996714	36.0	36.0	36.0	32.0	36.0
28	34.76219213441522	36.0	36.0	36.0	32.0	36.0
29	34.749584732496025	36.0	36.0	36.0	32.0	36.0
30	34.70138980174711	36.0	36.0	36.0	32.0	36.0
31	34.708356735698665	36.0	36.0	36.0	32.0	36.0
32	34.672291201108976	36.0	36.0	36.0	32.0	36.0
33	34.66969801986114	36.0	36.0	36.0	32.0	36.0
34	34.658871189397836	36.0	36.0	36.0	32.0	36.0
35	34.62783666543182	36.0	36.0	36.0	32.0	36.0
36	34.57780141250702	36.0	36.0	36.0	32.0	36.0
37	34.576522747099105	36.0	36.0	36.0	32.0	36.0
38	34.17468720498082	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	8.0
23	16.0
24	54.0
25	144.0
26	261.0
27	488.0
28	912.0
29	1413.0
30	2172.0
31	3015.0
32	4317.0
33	6665.0
34	16812.0
35	47402.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.368677693852185	19.314705038722632	13.132230614781529	26.184386652643653
2	15.338009823018368	21.123074533048122	37.535402301597735	26.003513342335776
3	18.093713029242004	24.38187880163956	29.817999306891647	27.706408862226787
4	12.124616101624024	17.711308421266477	36.25673689367957	33.90733858342993
5	13.700840095123146	36.87216930964018	34.44629007779544	14.980700517441234
6	32.32991957553089	36.841098935242165	17.289468338093474	13.53951315113347
7	28.08044836940285	31.05125416761272	21.828133029003	19.04016443398143
8	26.43610855510809	34.75101874977594	20.155112869110074	18.657759826005904
9	25.862542873190957	14.676673398902922	19.44739892682577	40.01338480108034
10	14.967734225621415	27.43427342256214	34.53035372848948	23.06763862332696
11	36.09670403212314	22.138435431057147	22.548340065489135	19.216520471330576
12	22.41395793499044	23.789435946462714	30.6453154875717	23.151290630975144
13	29.498087954110897	20.89746653919694	25.66802103250478	23.936424474187383
14	22.38142469616759	19.23017172356927	26.672721406292947	31.715682173970194
15	24.73918810721669	26.95952486227459	23.638579844887133	24.66270718562159
16	24.421314276837037	26.837633393482392	24.441629521635736	24.299422808044838
17	22.472245790561775	26.507809419103502	26.449253713507247	24.570691076827476
18	23.24781013611214	27.163872324661515	25.873256772744114	23.715060766482235
19	24.355588484841242	25.942567607939676	26.544854865501126	23.156989041717953
20	24.743968164816383	25.75136530395191	26.22937106392132	23.275295467310382
21	25.59123337436216	25.25065427038396	25.471731934369807	23.68638042088407
22	24.44640957923543	25.60796357596109	25.731050059153215	24.214576785650266
23	24.390243902439025	26.167230315125295	25.840991383946175	23.601534398489502
24	23.76525137127902	26.3859179503113	25.58645331676247	24.262377361647207
25	24.546790788829007	25.39644602717463	26.25207633751987	23.8046868464765
26	24.072370072059368	25.77287556315054	25.89954708954243	24.255207275247667
27	23.85965750887298	26.44447365590755	26.097919479929732	23.59794935528973
28	24.081930187258756	25.6187187055604	25.928227435140595	24.371123672040248
29	23.774811486478413	26.38113789271161	25.854136542345334	23.989914078464643
30	23.91701820006931	26.023828587134474	25.097692427193746	24.961460785602466
31	24.118975633656387	25.97722302553746	25.89954708954243	24.004254251263728
32	23.2203248049139	26.485104145504952	26.24251622232048	24.05205482726067
33	23.527443505694244	26.013073457535164	26.327362244715047	24.132120792055545
34	24.657927128021893	24.91485522400545	26.57353521109929	23.853682436873363
35	23.932553387268314	26.145720055926674	26.268806539118795	23.652920017686213
36	24.308982923244223	25.29486980318113	25.75017028955199	24.645976984022656
37	23.56687898089172	25.59720844636178	26.442083627107703	24.393828945638795
38	23.47486287209761	25.1968786223874	26.352457547113445	24.975800958401546
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	5.0
2	8.0
3	8.0
4	18.0
5	28.0
6	28.0
7	28.5
8	29.0
9	27.5
10	26.0
11	26.0
12	48.0
13	70.0
14	124.0
15	178.0
16	178.0
17	280.5
18	383.0
19	383.0
20	456.0
21	529.0
22	640.5
23	752.0
24	752.0
25	975.0
26	1198.0
27	1198.0
28	1684.5
29	2171.0
30	2706.0
31	3241.0
32	3241.0
33	3751.5
34	4262.0
35	4262.0
36	4540.5
37	4819.0
38	5127.5
39	5436.0
40	5436.0
41	6038.0
42	6640.0
43	6657.0
44	6674.0
45	6674.0
46	7128.5
47	7583.0
48	7583.0
49	7409.0
50	7235.0
51	6893.5
52	6552.0
53	6552.0
54	6325.5
55	6099.0
56	6099.0
57	5428.0
58	4757.0
59	4124.0
60	3491.0
61	3491.0
62	3124.0
63	2757.0
64	2497.0
65	2237.0
66	2237.0
67	2011.5
68	1786.0
69	1786.0
70	1664.5
71	1543.0
72	1335.0
73	1127.0
74	1127.0
75	1038.0
76	949.0
77	949.0
78	817.0
79	685.0
80	484.0
81	283.0
82	283.0
83	207.5
84	132.0
85	132.0
86	73.0
87	14.0
88	9.5
89	5.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010755129599311672
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.004780057599694076
10	0.001195014399923519
11	0.003585043199770557
12	0.001195014399923519
13	0.001195014399923519
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	83681.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.66207382798963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.8256123873588	21.025083352254395
2	16.0585099631933	12.095935756025861
3	8.627363878664806	9.747732460176145
4	5.31158776494479	8.001816421887883
5	3.4902906460210685	6.572579199579355
6	2.341667724330499	5.291523762861342
7	1.7514913060033	4.617535641304478
8	1.3231374539916234	3.9865680381448594
9	1.0502601853027034	3.5599478973721634
>10	4.197867749714431	24.273132491246518
>50	0.012691965985531158	0.3202638591795031
>100	0.009518974489148369	0.5078811199674956
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	194	0.23183279358516268	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	116	0.13862167039112822	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACAT	115	0.13742665599120468	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.001195014399923519	0.0
18	0.0	0.0	0.0	0.001195014399923519	0.0
19	0.0	0.0	0.0	0.001195014399923519	0.0
20	0.0	0.0	0.0	0.001195014399923519	0.0
21	0.0	0.0	0.0	0.001195014399923519	0.0
22	0.0	0.0	0.0	0.002390028799847038	0.0
23	0.0	0.0	0.0	0.002390028799847038	0.0
24	0.0	0.0	0.0	0.0035850431997705573	0.0
25	0.0	0.0	0.0	0.0035850431997705573	0.0
26	0.0	0.0	0.0	0.0035850431997705573	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGAC	20	0.0037252712	32.01495	3
ACACTCC	20	0.0037252712	32.01495	6
TTATACA	20	0.003736206	31.995815	2
CTTATAC	20	0.003736206	31.995815	1
GGTATCA	20	0.003736206	31.995815	1
TCGTAGA	20	0.003736206	31.995815	2
TATACAC	35	6.34053E-5	27.44139	3
GTATCAA	70	1.0841177E-9	25.13957	1
TAAGACC	40	0.004437382	20.009344	4
ATACACA	40	0.004437382	20.009344	4
CTCTTAG	40	0.0044527855	19.997385	1
TCTAAGA	40	0.0044527855	19.997385	2
ATGGGGG	45	0.008760128	17.786083	5
CTGTCTC	45	0.008760128	17.786083	9
TAGGGAT	45	0.008760128	17.786083	4
CAACGCA	120	5.216734E-7	14.67352	5
TCAACGC	120	5.216734E-7	14.67352	4
TACACAT	70	0.006713768	13.720695	5
ATCAACG	145	3.017667E-7	13.247567	3
GTACATG	190	3.781679E-9	12.629927	1
>>END_MODULE